Penetration of Steinernematid nematodes (Nematoda: Steinernematidae) into Japanese beetle larvae, Popillia japonica (Coleoptera: Scarabaeidae)

Liwang Cui, Randy Gaugler, Yi Wang

Research output: Contribution to journalArticle

29 Citations (Scopus)

Abstract

Penetration of infective juveniles of Steinernema glaseri and Steinernema carpocapsae into Japanese beetle larvae, Popillia japonica, was compared using external exposure and injection methods. Steinernematids entered the grubs through the mouth and anus, but not through the spiracles or cuticle. For all treatments, S. glaseri had higher levels of penetration than S. carpocapsae. Penetration of the gut by orally injected nematodes began 4-6 hr postinoculation. All regions of the gut were penetrated, with more than 82% of all penetrating nematodes invading the host hemocoel via the midgut. Gastric cecae were important sites for nematode penetration. Penetration sites of anally injected nematodes were the rectal sac of the hindgut. Penetration rates of isolated guts and that in vivo did not differ. Defecation by P. japonica was rapid, with orally injected nematodes appearing in the feces in 4-8 hr. Our results indicate that compared with S. glaseri, S. carpocapsae is poorly adapted to parasitize Japanese beetle larvae.

Original languageEnglish (US)
Pages (from-to)73-78
Number of pages6
JournalJournal of invertebrate pathology
Volume62
Issue number1
DOIs
StatePublished - Jan 1 1993

Fingerprint

Popillia japonica
Steinernematidae
Scarabaeidae
nematode
Steinernema glaseri
insect larvae
beetle
penetration
Steinernema carpocapsae
Nematoda
Coleoptera
larva
digestive system
spiracles
hemocoel
defecation
anus
nematode larvae
hindgut
midgut

All Science Journal Classification (ASJC) codes

  • Ecology, Evolution, Behavior and Systematics

Cite this

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title = "Penetration of Steinernematid nematodes (Nematoda: Steinernematidae) into Japanese beetle larvae, Popillia japonica (Coleoptera: Scarabaeidae)",
abstract = "Penetration of infective juveniles of Steinernema glaseri and Steinernema carpocapsae into Japanese beetle larvae, Popillia japonica, was compared using external exposure and injection methods. Steinernematids entered the grubs through the mouth and anus, but not through the spiracles or cuticle. For all treatments, S. glaseri had higher levels of penetration than S. carpocapsae. Penetration of the gut by orally injected nematodes began 4-6 hr postinoculation. All regions of the gut were penetrated, with more than 82{\%} of all penetrating nematodes invading the host hemocoel via the midgut. Gastric cecae were important sites for nematode penetration. Penetration sites of anally injected nematodes were the rectal sac of the hindgut. Penetration rates of isolated guts and that in vivo did not differ. Defecation by P. japonica was rapid, with orally injected nematodes appearing in the feces in 4-8 hr. Our results indicate that compared with S. glaseri, S. carpocapsae is poorly adapted to parasitize Japanese beetle larvae.",
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Penetration of Steinernematid nematodes (Nematoda : Steinernematidae) into Japanese beetle larvae, Popillia japonica (Coleoptera: Scarabaeidae). / Cui, Liwang; Gaugler, Randy; Wang, Yi.

In: Journal of invertebrate pathology, Vol. 62, No. 1, 01.01.1993, p. 73-78.

Research output: Contribution to journalArticle

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