Penta-O-galloyl-beta-D-glucose induces S- and G1-cell cycle arrests in prostate cancer cells targeting DNA replication and cyclin D1

Hongbo Hu, Jinhui Zhang, Hyo Jeong Lee, Sung Hoon Kim, Junxuan Lü

Research output: Contribution to journalArticle

37 Citations (Scopus)

Abstract

We have recently shown that penta-1,2,3,4,6-O-galloyl-beta-D-glucose (PGG), a naturally occurring hydrolyzable gallotannin, inhibited the in vivo growth of human androgen-independent p53-mutant DU145 prostate cancer (PCa) xenograft in athymic nude mice without adverse effect on their body weight. We have also shown that PGG induced caspase-mediated apoptosis in the DU145 cells and the androgen-dependent human p53-wild-type LNCaP cells. Here, we investigated the cell cycle effects of PGG in these and other PCa cells. Our data show that treatment with subapoptotic doses of PGG induced S-arrest, whereas higher doses of PGG induced not only S-arrest but also G1 arrest. We show, for the first time, that irrespective of the p53 functional status of the PCa cell lines, PGG exerted a rapid (within 2 h) and potent inhibition (inhibitory concentration by 50% ∼6 μM) of 5-bromo-2′-deoxyuridine incorporation into S phase cells. In isolated nuclei, PGG inhibited DNA replicative synthesis with superior efficacy than a known DNA polymerase alpha inhibitor, aphidocolin. In addition to the S-arrest action, we have found a close association of downregulation of cyclin D1 with G1 arrest induced by PGG. Overexpressing this G1 cyclin abolished G1 arrest, but hastened the S-arrest induction by PGG. Together, our data indicate that PGG induced PCa S-arrest probably through DNA replicative blockage and induced G1 arrest via cyclin D1 downregulation to contribute to anticancer activity. Our data raise the hypothesis that PGG may be a novel inhibitor of DNA polymerases.

Original languageEnglish (US)
Pages (from-to)818-823
Number of pages6
JournalCarcinogenesis
Volume30
Issue number5
DOIs
StatePublished - May 13 2009

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Prostaglandins G
G1 Phase Cell Cycle Checkpoints
Rubiaceae
Cyclin D1
DNA Replication
Prostatic Neoplasms
Glucose
Nucleic Acid Synthesis Inhibitors
Nude Mice
Androgens
Down-Regulation
Cyclin G1
Hydrolyzable Tannins
DNA Polymerase I
DNA
Bromodeoxyuridine
Caspases
S Phase
Heterografts

All Science Journal Classification (ASJC) codes

  • Cancer Research

Cite this

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title = "Penta-O-galloyl-beta-D-glucose induces S- and G1-cell cycle arrests in prostate cancer cells targeting DNA replication and cyclin D1",
abstract = "We have recently shown that penta-1,2,3,4,6-O-galloyl-beta-D-glucose (PGG), a naturally occurring hydrolyzable gallotannin, inhibited the in vivo growth of human androgen-independent p53-mutant DU145 prostate cancer (PCa) xenograft in athymic nude mice without adverse effect on their body weight. We have also shown that PGG induced caspase-mediated apoptosis in the DU145 cells and the androgen-dependent human p53-wild-type LNCaP cells. Here, we investigated the cell cycle effects of PGG in these and other PCa cells. Our data show that treatment with subapoptotic doses of PGG induced S-arrest, whereas higher doses of PGG induced not only S-arrest but also G1 arrest. We show, for the first time, that irrespective of the p53 functional status of the PCa cell lines, PGG exerted a rapid (within 2 h) and potent inhibition (inhibitory concentration by 50{\%} ∼6 μM) of 5-bromo-2′-deoxyuridine incorporation into S phase cells. In isolated nuclei, PGG inhibited DNA replicative synthesis with superior efficacy than a known DNA polymerase alpha inhibitor, aphidocolin. In addition to the S-arrest action, we have found a close association of downregulation of cyclin D1 with G1 arrest induced by PGG. Overexpressing this G1 cyclin abolished G1 arrest, but hastened the S-arrest induction by PGG. Together, our data indicate that PGG induced PCa S-arrest probably through DNA replicative blockage and induced G1 arrest via cyclin D1 downregulation to contribute to anticancer activity. Our data raise the hypothesis that PGG may be a novel inhibitor of DNA polymerases.",
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Penta-O-galloyl-beta-D-glucose induces S- and G1-cell cycle arrests in prostate cancer cells targeting DNA replication and cyclin D1. / Hu, Hongbo; Zhang, Jinhui; Lee, Hyo Jeong; Kim, Sung Hoon; Lü, Junxuan.

In: Carcinogenesis, Vol. 30, No. 5, 13.05.2009, p. 818-823.

Research output: Contribution to journalArticle

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AU - Lü, Junxuan

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