Perfluoroalkyl substance exposure and urine CC16 levels among asthmatics

A case–control study of children

Yang Zhou, Wen Wen Bao, Zhengmin Qian, Sarah Dee Geiger, Katelyn L. Parrish, Bo Yi Yang, Yungling Leo Lee, Guang Hui Dong

Research output: Contribution to journalArticle

Abstract

Background Studies have reported an association between serum perfluoroalkyl substances (PFASs) and asthma. However, few studies have examined the possible associations between PFASs and the 16-kDa club cell secretory protein (Clara) (CC16) level, a prominent biomarker of asthma, among adolescents. Methods We recruited a total of 231 asthmatic children and 225 non-asthmatic controls in the Genetic and Biomarkers study for Childhood Asthma (GBCA) in northern Taiwan from 2009 to 2010. Structured questionnaires were administered by face-to-face interview. Urine CC16 was determined by an enzyme-link immunoassay kit. Multiple general linear models were employed to examine the associations between PFASs and urinary CC16 levels. Results Asthmatic participants had significantly higher serum PFAS concentrations overall than the healthy controls. After adjusting for confounding factors, urinary CC16 was significantly, negatively associated with PFASs, especially PFOS, PFOA, PFDA and PFNA, and especially among males, as follows: PFOS (β = −0.003, 95% confidence interval [CI]: −0.004, −0.002), PFOA (β = −0.045, 95% CI: −0.086, −0.004), and PFHxA (β = −0.310, 95% CI: −0.455, −0.165) among asthmatic boys, and PFDA (β = −0.126, 95%CI: −0.241, −0.012) and PFNA (β = −0.329, 95% CI: −0.526, −0.132) among non-asthmatic boys. Among girls, PFDA (β = −0.088, 95% CI: −0.172, −0.004), was the only PFAS significantly associated with CC16. Significant interaction effects (p < 0.15) on CC16 levels were found between asthma and PFOS, PFOA, PFBS and PFHxA in all participants. Conclusion Our overall results showed that serum PFASs were significantly, inversely associated with CC16 levels. Associations were stronger among males.

Original languageEnglish (US)
Pages (from-to)158-163
Number of pages6
JournalEnvironmental Research
Volume159
DOIs
StatePublished - Jan 1 2017

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Biomarkers
urine
confidence interval
asthma
Uteroglobin
Urine
Confidence Intervals
Asthma
serum
Association reactions
biomarker
Enzymes
Serum
immunoassay
Immunoenzyme Techniques
Taiwan
exposure
Linear Models
Interviews
enzyme

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Environmental Science(all)

Cite this

Zhou, Yang ; Bao, Wen Wen ; Qian, Zhengmin ; Dee Geiger, Sarah ; Parrish, Katelyn L. ; Yang, Bo Yi ; Lee, Yungling Leo ; Dong, Guang Hui. / Perfluoroalkyl substance exposure and urine CC16 levels among asthmatics : A case–control study of children. In: Environmental Research. 2017 ; Vol. 159. pp. 158-163.
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title = "Perfluoroalkyl substance exposure and urine CC16 levels among asthmatics: A case–control study of children",
abstract = "Background Studies have reported an association between serum perfluoroalkyl substances (PFASs) and asthma. However, few studies have examined the possible associations between PFASs and the 16-kDa club cell secretory protein (Clara) (CC16) level, a prominent biomarker of asthma, among adolescents. Methods We recruited a total of 231 asthmatic children and 225 non-asthmatic controls in the Genetic and Biomarkers study for Childhood Asthma (GBCA) in northern Taiwan from 2009 to 2010. Structured questionnaires were administered by face-to-face interview. Urine CC16 was determined by an enzyme-link immunoassay kit. Multiple general linear models were employed to examine the associations between PFASs and urinary CC16 levels. Results Asthmatic participants had significantly higher serum PFAS concentrations overall than the healthy controls. After adjusting for confounding factors, urinary CC16 was significantly, negatively associated with PFASs, especially PFOS, PFOA, PFDA and PFNA, and especially among males, as follows: PFOS (β = −0.003, 95{\%} confidence interval [CI]: −0.004, −0.002), PFOA (β = −0.045, 95{\%} CI: −0.086, −0.004), and PFHxA (β = −0.310, 95{\%} CI: −0.455, −0.165) among asthmatic boys, and PFDA (β = −0.126, 95{\%}CI: −0.241, −0.012) and PFNA (β = −0.329, 95{\%} CI: −0.526, −0.132) among non-asthmatic boys. Among girls, PFDA (β = −0.088, 95{\%} CI: −0.172, −0.004), was the only PFAS significantly associated with CC16. Significant interaction effects (p < 0.15) on CC16 levels were found between asthma and PFOS, PFOA, PFBS and PFHxA in all participants. Conclusion Our overall results showed that serum PFASs were significantly, inversely associated with CC16 levels. Associations were stronger among males.",
author = "Yang Zhou and Bao, {Wen Wen} and Zhengmin Qian and {Dee Geiger}, Sarah and Parrish, {Katelyn L.} and Yang, {Bo Yi} and Lee, {Yungling Leo} and Dong, {Guang Hui}",
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Perfluoroalkyl substance exposure and urine CC16 levels among asthmatics : A case–control study of children. / Zhou, Yang; Bao, Wen Wen; Qian, Zhengmin; Dee Geiger, Sarah; Parrish, Katelyn L.; Yang, Bo Yi; Lee, Yungling Leo; Dong, Guang Hui.

In: Environmental Research, Vol. 159, 01.01.2017, p. 158-163.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Perfluoroalkyl substance exposure and urine CC16 levels among asthmatics

T2 - A case–control study of children

AU - Zhou, Yang

AU - Bao, Wen Wen

AU - Qian, Zhengmin

AU - Dee Geiger, Sarah

AU - Parrish, Katelyn L.

AU - Yang, Bo Yi

AU - Lee, Yungling Leo

AU - Dong, Guang Hui

PY - 2017/1/1

Y1 - 2017/1/1

N2 - Background Studies have reported an association between serum perfluoroalkyl substances (PFASs) and asthma. However, few studies have examined the possible associations between PFASs and the 16-kDa club cell secretory protein (Clara) (CC16) level, a prominent biomarker of asthma, among adolescents. Methods We recruited a total of 231 asthmatic children and 225 non-asthmatic controls in the Genetic and Biomarkers study for Childhood Asthma (GBCA) in northern Taiwan from 2009 to 2010. Structured questionnaires were administered by face-to-face interview. Urine CC16 was determined by an enzyme-link immunoassay kit. Multiple general linear models were employed to examine the associations between PFASs and urinary CC16 levels. Results Asthmatic participants had significantly higher serum PFAS concentrations overall than the healthy controls. After adjusting for confounding factors, urinary CC16 was significantly, negatively associated with PFASs, especially PFOS, PFOA, PFDA and PFNA, and especially among males, as follows: PFOS (β = −0.003, 95% confidence interval [CI]: −0.004, −0.002), PFOA (β = −0.045, 95% CI: −0.086, −0.004), and PFHxA (β = −0.310, 95% CI: −0.455, −0.165) among asthmatic boys, and PFDA (β = −0.126, 95%CI: −0.241, −0.012) and PFNA (β = −0.329, 95% CI: −0.526, −0.132) among non-asthmatic boys. Among girls, PFDA (β = −0.088, 95% CI: −0.172, −0.004), was the only PFAS significantly associated with CC16. Significant interaction effects (p < 0.15) on CC16 levels were found between asthma and PFOS, PFOA, PFBS and PFHxA in all participants. Conclusion Our overall results showed that serum PFASs were significantly, inversely associated with CC16 levels. Associations were stronger among males.

AB - Background Studies have reported an association between serum perfluoroalkyl substances (PFASs) and asthma. However, few studies have examined the possible associations between PFASs and the 16-kDa club cell secretory protein (Clara) (CC16) level, a prominent biomarker of asthma, among adolescents. Methods We recruited a total of 231 asthmatic children and 225 non-asthmatic controls in the Genetic and Biomarkers study for Childhood Asthma (GBCA) in northern Taiwan from 2009 to 2010. Structured questionnaires were administered by face-to-face interview. Urine CC16 was determined by an enzyme-link immunoassay kit. Multiple general linear models were employed to examine the associations between PFASs and urinary CC16 levels. Results Asthmatic participants had significantly higher serum PFAS concentrations overall than the healthy controls. After adjusting for confounding factors, urinary CC16 was significantly, negatively associated with PFASs, especially PFOS, PFOA, PFDA and PFNA, and especially among males, as follows: PFOS (β = −0.003, 95% confidence interval [CI]: −0.004, −0.002), PFOA (β = −0.045, 95% CI: −0.086, −0.004), and PFHxA (β = −0.310, 95% CI: −0.455, −0.165) among asthmatic boys, and PFDA (β = −0.126, 95%CI: −0.241, −0.012) and PFNA (β = −0.329, 95% CI: −0.526, −0.132) among non-asthmatic boys. Among girls, PFDA (β = −0.088, 95% CI: −0.172, −0.004), was the only PFAS significantly associated with CC16. Significant interaction effects (p < 0.15) on CC16 levels were found between asthma and PFOS, PFOA, PFBS and PFHxA in all participants. Conclusion Our overall results showed that serum PFASs were significantly, inversely associated with CC16 levels. Associations were stronger among males.

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