Performance of two rapid diagnostic tests for malaria diagnosis at the China-Myanmar border area

Juan Yan, Nana Li, Xu Wei, Peipei Li, Zhenjun Zhao, Lili Wang, Siying Li, Xiaomei Li, Ying Wang, Shuying Li, Zhaoqing Yang, Bin Zheng, Guofa Zhou, Guiyun Yan, Liwang Cui, Yaming Cao, Qi Fan

Research output: Contribution to journalArticle

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Abstract

Background: Rapid diagnostic tests (RDTs) have become an essential tool in the contemporary malaria control and management programmes in the world. This study aims to evaluate the performance of two commonly used RDTs for malaria diagnosis in the China-Myanmar border area. Methods. A total 606 febrile patients in the China-Myanmar border were recruited to this study and were diagnosed for malaria infections by microscopy, two RDTs tests (Pf/Pan device, and Pv/Pf device) and nested PCR. Results: Malaria parasites were found in 143 patients by microscopy, of which 51, 73, and 19 were Plasmodium falciparum, Plasmodium vivax and P. falciparum/P. vivax mixed infections, respectively. Compared to microscopy, the sensitivity of the Pf/Pan device was 88.6% for P. falciparum and 69.9% for P. vivax with the specificity of 90.4%. For a subset of 350 patients, the sensitivity of the Pf/Pan device and Pv/Pf device for detection of P. falciparum was 87.5% and 91.7%, respectively; and for detection of P. vivax was 72.0% and 73.8%, respectively. The specificity of the Pf/Pan device and Pv/Pf device was 94.3% and 96.5%, respectively. Nested PCR detected malaria parasites in 174 of 606 samples, of which 67, 79, two and 26 were P. falciparum, P. vivax, P. ovale and P. falciparum/P. vivax mixed infections, respectively. Compared to nested PCR, all other methods had sensitivity below 80%, suggesting that a significant number of cases were missed. Conclusions: Compared to PCR, both microscopy and RDTs had lower sensitivities. RDTs had similar performance to microscopy for P. falciparum diagnosis, but performed worse for P. vivax diagnosis. Other RDT products should be selected with higher sensitivity (and good specificity) for both P. falciparum and P. vivax diagnosis.

Original languageEnglish (US)
Article number73
JournalMalaria journal
Volume12
Issue number1
DOIs
StatePublished - Feb 26 2013

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Myanmar
Plasmodium vivax
Plasmodium falciparum
Routine Diagnostic Tests
Malaria
China
Equipment and Supplies
Microscopy
Polymerase Chain Reaction
Coinfection
Parasites
Fever
Sensitivity and Specificity

All Science Journal Classification (ASJC) codes

  • Parasitology
  • Infectious Diseases

Cite this

Yan, Juan ; Li, Nana ; Wei, Xu ; Li, Peipei ; Zhao, Zhenjun ; Wang, Lili ; Li, Siying ; Li, Xiaomei ; Wang, Ying ; Li, Shuying ; Yang, Zhaoqing ; Zheng, Bin ; Zhou, Guofa ; Yan, Guiyun ; Cui, Liwang ; Cao, Yaming ; Fan, Qi. / Performance of two rapid diagnostic tests for malaria diagnosis at the China-Myanmar border area. In: Malaria journal. 2013 ; Vol. 12, No. 1.
@article{ad36673acb8c478bbeb37ea9d22f5398,
title = "Performance of two rapid diagnostic tests for malaria diagnosis at the China-Myanmar border area",
abstract = "Background: Rapid diagnostic tests (RDTs) have become an essential tool in the contemporary malaria control and management programmes in the world. This study aims to evaluate the performance of two commonly used RDTs for malaria diagnosis in the China-Myanmar border area. Methods. A total 606 febrile patients in the China-Myanmar border were recruited to this study and were diagnosed for malaria infections by microscopy, two RDTs tests (Pf/Pan device, and Pv/Pf device) and nested PCR. Results: Malaria parasites were found in 143 patients by microscopy, of which 51, 73, and 19 were Plasmodium falciparum, Plasmodium vivax and P. falciparum/P. vivax mixed infections, respectively. Compared to microscopy, the sensitivity of the Pf/Pan device was 88.6{\%} for P. falciparum and 69.9{\%} for P. vivax with the specificity of 90.4{\%}. For a subset of 350 patients, the sensitivity of the Pf/Pan device and Pv/Pf device for detection of P. falciparum was 87.5{\%} and 91.7{\%}, respectively; and for detection of P. vivax was 72.0{\%} and 73.8{\%}, respectively. The specificity of the Pf/Pan device and Pv/Pf device was 94.3{\%} and 96.5{\%}, respectively. Nested PCR detected malaria parasites in 174 of 606 samples, of which 67, 79, two and 26 were P. falciparum, P. vivax, P. ovale and P. falciparum/P. vivax mixed infections, respectively. Compared to nested PCR, all other methods had sensitivity below 80{\%}, suggesting that a significant number of cases were missed. Conclusions: Compared to PCR, both microscopy and RDTs had lower sensitivities. RDTs had similar performance to microscopy for P. falciparum diagnosis, but performed worse for P. vivax diagnosis. Other RDT products should be selected with higher sensitivity (and good specificity) for both P. falciparum and P. vivax diagnosis.",
author = "Juan Yan and Nana Li and Xu Wei and Peipei Li and Zhenjun Zhao and Lili Wang and Siying Li and Xiaomei Li and Ying Wang and Shuying Li and Zhaoqing Yang and Bin Zheng and Guofa Zhou and Guiyun Yan and Liwang Cui and Yaming Cao and Qi Fan",
year = "2013",
month = "2",
day = "26",
doi = "10.1186/1475-2875-12-73",
language = "English (US)",
volume = "12",
journal = "Malaria Journal",
issn = "1475-2875",
publisher = "BioMed Central",
number = "1",

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Yan, J, Li, N, Wei, X, Li, P, Zhao, Z, Wang, L, Li, S, Li, X, Wang, Y, Li, S, Yang, Z, Zheng, B, Zhou, G, Yan, G, Cui, L, Cao, Y & Fan, Q 2013, 'Performance of two rapid diagnostic tests for malaria diagnosis at the China-Myanmar border area', Malaria journal, vol. 12, no. 1, 73. https://doi.org/10.1186/1475-2875-12-73

Performance of two rapid diagnostic tests for malaria diagnosis at the China-Myanmar border area. / Yan, Juan; Li, Nana; Wei, Xu; Li, Peipei; Zhao, Zhenjun; Wang, Lili; Li, Siying; Li, Xiaomei; Wang, Ying; Li, Shuying; Yang, Zhaoqing; Zheng, Bin; Zhou, Guofa; Yan, Guiyun; Cui, Liwang; Cao, Yaming; Fan, Qi.

In: Malaria journal, Vol. 12, No. 1, 73, 26.02.2013.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Performance of two rapid diagnostic tests for malaria diagnosis at the China-Myanmar border area

AU - Yan, Juan

AU - Li, Nana

AU - Wei, Xu

AU - Li, Peipei

AU - Zhao, Zhenjun

AU - Wang, Lili

AU - Li, Siying

AU - Li, Xiaomei

AU - Wang, Ying

AU - Li, Shuying

AU - Yang, Zhaoqing

AU - Zheng, Bin

AU - Zhou, Guofa

AU - Yan, Guiyun

AU - Cui, Liwang

AU - Cao, Yaming

AU - Fan, Qi

PY - 2013/2/26

Y1 - 2013/2/26

N2 - Background: Rapid diagnostic tests (RDTs) have become an essential tool in the contemporary malaria control and management programmes in the world. This study aims to evaluate the performance of two commonly used RDTs for malaria diagnosis in the China-Myanmar border area. Methods. A total 606 febrile patients in the China-Myanmar border were recruited to this study and were diagnosed for malaria infections by microscopy, two RDTs tests (Pf/Pan device, and Pv/Pf device) and nested PCR. Results: Malaria parasites were found in 143 patients by microscopy, of which 51, 73, and 19 were Plasmodium falciparum, Plasmodium vivax and P. falciparum/P. vivax mixed infections, respectively. Compared to microscopy, the sensitivity of the Pf/Pan device was 88.6% for P. falciparum and 69.9% for P. vivax with the specificity of 90.4%. For a subset of 350 patients, the sensitivity of the Pf/Pan device and Pv/Pf device for detection of P. falciparum was 87.5% and 91.7%, respectively; and for detection of P. vivax was 72.0% and 73.8%, respectively. The specificity of the Pf/Pan device and Pv/Pf device was 94.3% and 96.5%, respectively. Nested PCR detected malaria parasites in 174 of 606 samples, of which 67, 79, two and 26 were P. falciparum, P. vivax, P. ovale and P. falciparum/P. vivax mixed infections, respectively. Compared to nested PCR, all other methods had sensitivity below 80%, suggesting that a significant number of cases were missed. Conclusions: Compared to PCR, both microscopy and RDTs had lower sensitivities. RDTs had similar performance to microscopy for P. falciparum diagnosis, but performed worse for P. vivax diagnosis. Other RDT products should be selected with higher sensitivity (and good specificity) for both P. falciparum and P. vivax diagnosis.

AB - Background: Rapid diagnostic tests (RDTs) have become an essential tool in the contemporary malaria control and management programmes in the world. This study aims to evaluate the performance of two commonly used RDTs for malaria diagnosis in the China-Myanmar border area. Methods. A total 606 febrile patients in the China-Myanmar border were recruited to this study and were diagnosed for malaria infections by microscopy, two RDTs tests (Pf/Pan device, and Pv/Pf device) and nested PCR. Results: Malaria parasites were found in 143 patients by microscopy, of which 51, 73, and 19 were Plasmodium falciparum, Plasmodium vivax and P. falciparum/P. vivax mixed infections, respectively. Compared to microscopy, the sensitivity of the Pf/Pan device was 88.6% for P. falciparum and 69.9% for P. vivax with the specificity of 90.4%. For a subset of 350 patients, the sensitivity of the Pf/Pan device and Pv/Pf device for detection of P. falciparum was 87.5% and 91.7%, respectively; and for detection of P. vivax was 72.0% and 73.8%, respectively. The specificity of the Pf/Pan device and Pv/Pf device was 94.3% and 96.5%, respectively. Nested PCR detected malaria parasites in 174 of 606 samples, of which 67, 79, two and 26 were P. falciparum, P. vivax, P. ovale and P. falciparum/P. vivax mixed infections, respectively. Compared to nested PCR, all other methods had sensitivity below 80%, suggesting that a significant number of cases were missed. Conclusions: Compared to PCR, both microscopy and RDTs had lower sensitivities. RDTs had similar performance to microscopy for P. falciparum diagnosis, but performed worse for P. vivax diagnosis. Other RDT products should be selected with higher sensitivity (and good specificity) for both P. falciparum and P. vivax diagnosis.

UR - http://www.scopus.com/inward/record.url?scp=84874049391&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84874049391&partnerID=8YFLogxK

U2 - 10.1186/1475-2875-12-73

DO - 10.1186/1475-2875-12-73

M3 - Article

VL - 12

JO - Malaria Journal

JF - Malaria Journal

SN - 1475-2875

IS - 1

M1 - 73

ER -