Periadventitial adipose-derived stem cell treatment halts elastase-induced abdominal aortic aneurysm progression

Kory Blose, Terri L. Ennis, Batool Arif, Justin S. Weinbaum, John A. Curci, David A. Vorp

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Aim: Demonstrate that periadventitial delivery of adipose-derived mesenchymal stem cells (ADMSCs) slows aneurysm progression in an established murine elastase-perfusion model of abdominal aortic aneurysm (AAA).

Materials & methods: AAAs were induced in C57BL/6 mice using porcine elastase. During elastase perfusion, a delivery device consisting of a subcutaneous port, tubing and porous scaffold was implanted. Five days after elastase perfusion, 100,000 ADMSCs were delivered through the port to the aorta. After sacrifice at day 14, analyzed metrics included aortic diameter and structure of aortic elastin.

Results: ADMSC treated aneurysms had a smaller diameter and less fragmented elastin versus saline controls.

Conclusion: Periadventitial stem cell delivery prevented the expansion of an established aneurysm between days 5 and 14 after elastase perfusion.

Original languageEnglish (US)
Pages (from-to)733-741
Number of pages9
JournalRegenerative Medicine
Volume9
Issue number6
DOIs
StatePublished - Jan 1 2014

Fingerprint

Pancreatic Elastase
Abdominal Aortic Aneurysm
Stem cells
Stem Cells
Elastin
Perfusion
Mesenchymal Stromal Cells
Aneurysm
Tubing
Scaffolds
Inbred C57BL Mouse
Aorta
Swine
Equipment and Supplies

All Science Journal Classification (ASJC) codes

  • Embryology
  • Biomedical Engineering

Cite this

Blose, Kory ; Ennis, Terri L. ; Arif, Batool ; Weinbaum, Justin S. ; Curci, John A. ; Vorp, David A. / Periadventitial adipose-derived stem cell treatment halts elastase-induced abdominal aortic aneurysm progression. In: Regenerative Medicine. 2014 ; Vol. 9, No. 6. pp. 733-741.
@article{5c9e01fde3c44a8baed98f06888b89ad,
title = "Periadventitial adipose-derived stem cell treatment halts elastase-induced abdominal aortic aneurysm progression",
abstract = "Aim: Demonstrate that periadventitial delivery of adipose-derived mesenchymal stem cells (ADMSCs) slows aneurysm progression in an established murine elastase-perfusion model of abdominal aortic aneurysm (AAA).Materials & methods: AAAs were induced in C57BL/6 mice using porcine elastase. During elastase perfusion, a delivery device consisting of a subcutaneous port, tubing and porous scaffold was implanted. Five days after elastase perfusion, 100,000 ADMSCs were delivered through the port to the aorta. After sacrifice at day 14, analyzed metrics included aortic diameter and structure of aortic elastin.Results: ADMSC treated aneurysms had a smaller diameter and less fragmented elastin versus saline controls.Conclusion: Periadventitial stem cell delivery prevented the expansion of an established aneurysm between days 5 and 14 after elastase perfusion.",
author = "Kory Blose and Ennis, {Terri L.} and Batool Arif and Weinbaum, {Justin S.} and Curci, {John A.} and Vorp, {David A.}",
year = "2014",
month = "1",
day = "1",
doi = "10.2217/rme.14.61",
language = "English (US)",
volume = "9",
pages = "733--741",
journal = "Regenerative Medicine",
issn = "1746-0751",
publisher = "Future Medicine Ltd.",
number = "6",

}

Periadventitial adipose-derived stem cell treatment halts elastase-induced abdominal aortic aneurysm progression. / Blose, Kory; Ennis, Terri L.; Arif, Batool; Weinbaum, Justin S.; Curci, John A.; Vorp, David A.

In: Regenerative Medicine, Vol. 9, No. 6, 01.01.2014, p. 733-741.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Periadventitial adipose-derived stem cell treatment halts elastase-induced abdominal aortic aneurysm progression

AU - Blose, Kory

AU - Ennis, Terri L.

AU - Arif, Batool

AU - Weinbaum, Justin S.

AU - Curci, John A.

AU - Vorp, David A.

PY - 2014/1/1

Y1 - 2014/1/1

N2 - Aim: Demonstrate that periadventitial delivery of adipose-derived mesenchymal stem cells (ADMSCs) slows aneurysm progression in an established murine elastase-perfusion model of abdominal aortic aneurysm (AAA).Materials & methods: AAAs were induced in C57BL/6 mice using porcine elastase. During elastase perfusion, a delivery device consisting of a subcutaneous port, tubing and porous scaffold was implanted. Five days after elastase perfusion, 100,000 ADMSCs were delivered through the port to the aorta. After sacrifice at day 14, analyzed metrics included aortic diameter and structure of aortic elastin.Results: ADMSC treated aneurysms had a smaller diameter and less fragmented elastin versus saline controls.Conclusion: Periadventitial stem cell delivery prevented the expansion of an established aneurysm between days 5 and 14 after elastase perfusion.

AB - Aim: Demonstrate that periadventitial delivery of adipose-derived mesenchymal stem cells (ADMSCs) slows aneurysm progression in an established murine elastase-perfusion model of abdominal aortic aneurysm (AAA).Materials & methods: AAAs were induced in C57BL/6 mice using porcine elastase. During elastase perfusion, a delivery device consisting of a subcutaneous port, tubing and porous scaffold was implanted. Five days after elastase perfusion, 100,000 ADMSCs were delivered through the port to the aorta. After sacrifice at day 14, analyzed metrics included aortic diameter and structure of aortic elastin.Results: ADMSC treated aneurysms had a smaller diameter and less fragmented elastin versus saline controls.Conclusion: Periadventitial stem cell delivery prevented the expansion of an established aneurysm between days 5 and 14 after elastase perfusion.

UR - http://www.scopus.com/inward/record.url?scp=84913604854&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84913604854&partnerID=8YFLogxK

U2 - 10.2217/rme.14.61

DO - 10.2217/rme.14.61

M3 - Article

VL - 9

SP - 733

EP - 741

JO - Regenerative Medicine

JF - Regenerative Medicine

SN - 1746-0751

IS - 6

ER -