PERK promotes cancer cell proliferation and tumor growth by limiting oxidative DNA damage

E. Bobrovnikova-Marjon, C. Grigoriadou, D. Pytel, F. Zhang, J. Ye, C. Koumenis, Douglas R. Cavener, J. A. Diehl

Research output: Contribution to journalArticle

145 Citations (Scopus)

Abstract

To proliferate and expand in an environment with limited nutrients, cancer cells co-opt cellular regulatory pathways that facilitate adaptation and thereby maintain tumor growth and survival potential. The endoplasmic reticulum (ER) is uniquely positioned to sense nutrient deprivation stress and subsequently engage signaling pathways that promote adaptive strategies. As such, components of the ER stress-signaling pathway represent potential antineoplastic targets. However, recent investigations into the role of the ER resident protein kinase, RNA-dependent protein kinase (PKR)-like ER kinase (PERK) have paradoxically suggested both pro-and anti-tumorigenic properties. We have used animal models of mammary carcinoma to interrogate the contribution of PERK in the neoplastic process. The ablation of PERK in tumor cells resulted in impaired regeneration of intracellular antioxidants and accumulation of reactive oxygen species triggering oxidative DNA damage. Ultimately, PERK deficiency impeded progression through the cell cycle because of the activation of the DNA damage checkpoint. Our data reveal that PERK-dependent signaling is used during both tumor initiation and expansion to maintain redox homeostasis, thereby facilitating tumor growth.

Original languageEnglish (US)
Pages (from-to)3881-3895
Number of pages15
JournalOncogene
Volume29
Issue number27
DOIs
StatePublished - Jul 8 2010

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DNA Damage
Phosphotransferases
Cell Proliferation
Endoplasmic Reticulum
eIF-2 Kinase
Growth
Neoplasms
Animal Mammary Neoplasms
Neoplastic Processes
Food
Endoplasmic Reticulum Stress
Antineoplastic Agents
Protein Kinases
Oxidation-Reduction
Regeneration
Reactive Oxygen Species
Cell Cycle
Homeostasis
Animal Models
Antioxidants

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Genetics
  • Cancer Research

Cite this

Bobrovnikova-Marjon, E., Grigoriadou, C., Pytel, D., Zhang, F., Ye, J., Koumenis, C., ... Diehl, J. A. (2010). PERK promotes cancer cell proliferation and tumor growth by limiting oxidative DNA damage. Oncogene, 29(27), 3881-3895. https://doi.org/10.1038/onc.2010.153
Bobrovnikova-Marjon, E. ; Grigoriadou, C. ; Pytel, D. ; Zhang, F. ; Ye, J. ; Koumenis, C. ; Cavener, Douglas R. ; Diehl, J. A. / PERK promotes cancer cell proliferation and tumor growth by limiting oxidative DNA damage. In: Oncogene. 2010 ; Vol. 29, No. 27. pp. 3881-3895.
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Bobrovnikova-Marjon, E, Grigoriadou, C, Pytel, D, Zhang, F, Ye, J, Koumenis, C, Cavener, DR & Diehl, JA 2010, 'PERK promotes cancer cell proliferation and tumor growth by limiting oxidative DNA damage', Oncogene, vol. 29, no. 27, pp. 3881-3895. https://doi.org/10.1038/onc.2010.153

PERK promotes cancer cell proliferation and tumor growth by limiting oxidative DNA damage. / Bobrovnikova-Marjon, E.; Grigoriadou, C.; Pytel, D.; Zhang, F.; Ye, J.; Koumenis, C.; Cavener, Douglas R.; Diehl, J. A.

In: Oncogene, Vol. 29, No. 27, 08.07.2010, p. 3881-3895.

Research output: Contribution to journalArticle

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Bobrovnikova-Marjon E, Grigoriadou C, Pytel D, Zhang F, Ye J, Koumenis C et al. PERK promotes cancer cell proliferation and tumor growth by limiting oxidative DNA damage. Oncogene. 2010 Jul 8;29(27):3881-3895. https://doi.org/10.1038/onc.2010.153