Perseorangin: A natural pigment from avocado (Persea americana) seed

Emmanuel Hatzakis, Eugene P. Mazzola, Rachel M. Shegog, Gregory Ray Ziegler, Joshua D. Lambert

Research output: Contribution to journalArticle

Abstract

High consumer demand has led global food color manufacturers and food companies to dramatically increase the development and use of natural colors. We have previously reported that avocado (Persea americana) seeds, when crushed in the presence of air, develop a red-orange color in a polyphenol oxidase-dependent reaction. The objective of this study was to identify the major colored compound in colored avocado seed extract (CASE). Column chromatography and high performance liquid chromatography were used to isolate the most abundant colored compound in CASE. This compound, henceforth referred to as perseorangin, was a yellow-orange solid. Structural analysis was performed using high-resolution mass spectrometry, and infrared and nuclear magnetic resonance spectroscopy. We determined that perseorangin is a glycosylated benzotropone-containing compound with a molecular formula of C29H30O14. Liquid chromatography with electrospray ionization mass spectrometry-based metabolomic analysis of CASE and uncolored avocado seed extract showed that perseorangin was unique to CASE.

Original languageEnglish (US)
Pages (from-to)15-22
Number of pages8
JournalFood Chemistry
Volume293
DOIs
StatePublished - Sep 30 2019

Fingerprint

Persea
Persea americana
avocados
Pigments
seed extracts
Seed
Seeds
pigments
seeds
Color
Mass spectrometry
color
Catechol Oxidase
Electrospray ionization
Column chromatography
consumer demand
Liquid chromatography
Food
High performance liquid chromatography
metabolomics

All Science Journal Classification (ASJC) codes

  • Analytical Chemistry
  • Food Science

Cite this

Hatzakis, Emmanuel ; Mazzola, Eugene P. ; Shegog, Rachel M. ; Ziegler, Gregory Ray ; Lambert, Joshua D. / Perseorangin : A natural pigment from avocado (Persea americana) seed. In: Food Chemistry. 2019 ; Vol. 293. pp. 15-22.
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Perseorangin : A natural pigment from avocado (Persea americana) seed. / Hatzakis, Emmanuel; Mazzola, Eugene P.; Shegog, Rachel M.; Ziegler, Gregory Ray; Lambert, Joshua D.

In: Food Chemistry, Vol. 293, 30.09.2019, p. 15-22.

Research output: Contribution to journalArticle

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AU - Mazzola, Eugene P.

AU - Shegog, Rachel M.

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AU - Lambert, Joshua D.

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