Persistent reduced oxygen requirement following blood transfusion during recovery from hemorrhagic shock

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Abstract

Our study intended to determine the effects on oxygen uptake (V ˙O2) of restoring a normal rate of O2 delivery following blood transfusion (BT) after a severe hemorrhage (H). Spontaneously breathing urethane anesthetized rats were bled by removing 20ml/kg of blood over 30min. Rats were then infused with their own shed blood 15min after the end of H. At mid-perfusion, half of the rats received a unique infusion of the decoupling agent 2,4-dinitrophenol (DNP, 6mg/kg). V ˙O2 and arterial blood pressure (ABP) were continuously measured throughout the study, along with serial determination of blood lactate concentration [La]. Animals were euthanized 45min after the end of reperfusion; liver and lungs were further analyzed for early expression of oxidative stress gene using RT-PCR. Our bleeding protocol induced a significant decrease in ABP and increase in [La], while V ˙O2 dropped by half. The O2 deficit progressively accumulated during the period of bleeding reached -114±53ml/kg, just before blood transfusion. Despite the transfusion of blood, a significant O2 deficit persisted (-82±59ml/kg) 45min after reperfusion. This slow recovery of V ˙O2 was sped up by DNP injection, leading to a fast recovery of O2 deficit after reperfusion, becoming positive (+460±132ml/kg) by the end of the protocol, supporting the view that O2 supply is not the main controller of V ˙O2 dynamics after BT. Of note is that DNP also enhanced oxidative stress gene expression (up-regulation of NADPH oxidase 4 in the lung for instance). The mechanism of slow recovery of O2 requirement/demand following BT and the resulting effects on tissues exposed to relatively high O2 partial pressure are discussed.

Original languageEnglish (US)
Pages (from-to)39-46
Number of pages8
JournalRespiratory Physiology and Neurobiology
Volume215
DOIs
StatePublished - Aug 5 2015

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Hemorrhagic Shock
Blood Transfusion
Oxygen
Reperfusion
2,4-Dinitrophenol
Hemorrhage
Arterial Pressure
Oxidative Stress
Lung
Partial Pressure
NADPH Oxidase
Urethane
Gene Expression Regulation
Lactic Acid
Respiration
Up-Regulation
Perfusion
Polymerase Chain Reaction
Injections
Liver

All Science Journal Classification (ASJC) codes

  • Neuroscience(all)
  • Physiology
  • Pulmonary and Respiratory Medicine

Cite this

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title = "Persistent reduced oxygen requirement following blood transfusion during recovery from hemorrhagic shock",
abstract = "Our study intended to determine the effects on oxygen uptake (V ˙O2) of restoring a normal rate of O2 delivery following blood transfusion (BT) after a severe hemorrhage (H). Spontaneously breathing urethane anesthetized rats were bled by removing 20ml/kg of blood over 30min. Rats were then infused with their own shed blood 15min after the end of H. At mid-perfusion, half of the rats received a unique infusion of the decoupling agent 2,4-dinitrophenol (DNP, 6mg/kg). V ˙O2 and arterial blood pressure (ABP) were continuously measured throughout the study, along with serial determination of blood lactate concentration [La]. Animals were euthanized 45min after the end of reperfusion; liver and lungs were further analyzed for early expression of oxidative stress gene using RT-PCR. Our bleeding protocol induced a significant decrease in ABP and increase in [La], while V ˙O2 dropped by half. The O2 deficit progressively accumulated during the period of bleeding reached -114±53ml/kg, just before blood transfusion. Despite the transfusion of blood, a significant O2 deficit persisted (-82±59ml/kg) 45min after reperfusion. This slow recovery of V ˙O2 was sped up by DNP injection, leading to a fast recovery of O2 deficit after reperfusion, becoming positive (+460±132ml/kg) by the end of the protocol, supporting the view that O2 supply is not the main controller of V ˙O2 dynamics after BT. Of note is that DNP also enhanced oxidative stress gene expression (up-regulation of NADPH oxidase 4 in the lung for instance). The mechanism of slow recovery of O2 requirement/demand following BT and the resulting effects on tissues exposed to relatively high O2 partial pressure are discussed.",
author = "Philippe Haouzi and {Van de Louw}, Andry",
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T1 - Persistent reduced oxygen requirement following blood transfusion during recovery from hemorrhagic shock

AU - Haouzi, Philippe

AU - Van de Louw, Andry

PY - 2015/8/5

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N2 - Our study intended to determine the effects on oxygen uptake (V ˙O2) of restoring a normal rate of O2 delivery following blood transfusion (BT) after a severe hemorrhage (H). Spontaneously breathing urethane anesthetized rats were bled by removing 20ml/kg of blood over 30min. Rats were then infused with their own shed blood 15min after the end of H. At mid-perfusion, half of the rats received a unique infusion of the decoupling agent 2,4-dinitrophenol (DNP, 6mg/kg). V ˙O2 and arterial blood pressure (ABP) were continuously measured throughout the study, along with serial determination of blood lactate concentration [La]. Animals were euthanized 45min after the end of reperfusion; liver and lungs were further analyzed for early expression of oxidative stress gene using RT-PCR. Our bleeding protocol induced a significant decrease in ABP and increase in [La], while V ˙O2 dropped by half. The O2 deficit progressively accumulated during the period of bleeding reached -114±53ml/kg, just before blood transfusion. Despite the transfusion of blood, a significant O2 deficit persisted (-82±59ml/kg) 45min after reperfusion. This slow recovery of V ˙O2 was sped up by DNP injection, leading to a fast recovery of O2 deficit after reperfusion, becoming positive (+460±132ml/kg) by the end of the protocol, supporting the view that O2 supply is not the main controller of V ˙O2 dynamics after BT. Of note is that DNP also enhanced oxidative stress gene expression (up-regulation of NADPH oxidase 4 in the lung for instance). The mechanism of slow recovery of O2 requirement/demand following BT and the resulting effects on tissues exposed to relatively high O2 partial pressure are discussed.

AB - Our study intended to determine the effects on oxygen uptake (V ˙O2) of restoring a normal rate of O2 delivery following blood transfusion (BT) after a severe hemorrhage (H). Spontaneously breathing urethane anesthetized rats were bled by removing 20ml/kg of blood over 30min. Rats were then infused with their own shed blood 15min after the end of H. At mid-perfusion, half of the rats received a unique infusion of the decoupling agent 2,4-dinitrophenol (DNP, 6mg/kg). V ˙O2 and arterial blood pressure (ABP) were continuously measured throughout the study, along with serial determination of blood lactate concentration [La]. Animals were euthanized 45min after the end of reperfusion; liver and lungs were further analyzed for early expression of oxidative stress gene using RT-PCR. Our bleeding protocol induced a significant decrease in ABP and increase in [La], while V ˙O2 dropped by half. The O2 deficit progressively accumulated during the period of bleeding reached -114±53ml/kg, just before blood transfusion. Despite the transfusion of blood, a significant O2 deficit persisted (-82±59ml/kg) 45min after reperfusion. This slow recovery of V ˙O2 was sped up by DNP injection, leading to a fast recovery of O2 deficit after reperfusion, becoming positive (+460±132ml/kg) by the end of the protocol, supporting the view that O2 supply is not the main controller of V ˙O2 dynamics after BT. Of note is that DNP also enhanced oxidative stress gene expression (up-regulation of NADPH oxidase 4 in the lung for instance). The mechanism of slow recovery of O2 requirement/demand following BT and the resulting effects on tissues exposed to relatively high O2 partial pressure are discussed.

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