Pescadillo is essential for nucleolar assembly, ribosome biogenesis, and mammalian cell proliferation

Alexandra Lerch-Gaggl, Jamil Haque, Jixuan Li, Gang Ning, Paula Traktman, Stephen A. Duncan

Research output: Contribution to journalArticle

83 Citations (Scopus)

Abstract

Mutation of the zebrafish pescadillo gene blocks expansion of a number of tissues in the developing embryo, suggesting roles for its gene product in controlling cell proliferation. We report that levels of the pescadillo protein increase in rodent hepatocytes as they enter the cell cycle. Pescadillo protein localizes to distinct substructures of the interphase nucleus including nucleoli, the site of ribosome biogenesis. During mitosis pescadillo closely associates with the periphery of metaphase chromosomes and by late anaphase is associated with nucleolus-derived foci and prenucleolar bodies. Blastomeres in mouse embryos lacking pescadillo arrest at morula stages of development, the nucleoli fail to differentiate and accumulation of ribosomes is inhibited. We propose that in mammalian cells pescadillo is essential for ribosome biogenesis and nucleologenesis and that disruption to its function results in cell cycle arrest.

Original languageEnglish (US)
Pages (from-to)45347-45355
Number of pages9
JournalJournal of Biological Chemistry
Volume277
Issue number47
DOIs
StatePublished - Nov 22 2002

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Cell proliferation
Ribosomes
Cells
Cell Proliferation
Embryonic Structures
Genes
Morula
Blastomeres
Anaphase
Interphase
Zebrafish
Metaphase
Chromosomes
Cell Cycle Checkpoints
Mitosis
Hepatocytes
Rodentia
Cell Cycle
Proteins
Tissue

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Lerch-Gaggl, Alexandra ; Haque, Jamil ; Li, Jixuan ; Ning, Gang ; Traktman, Paula ; Duncan, Stephen A. / Pescadillo is essential for nucleolar assembly, ribosome biogenesis, and mammalian cell proliferation. In: Journal of Biological Chemistry. 2002 ; Vol. 277, No. 47. pp. 45347-45355.
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Pescadillo is essential for nucleolar assembly, ribosome biogenesis, and mammalian cell proliferation. / Lerch-Gaggl, Alexandra; Haque, Jamil; Li, Jixuan; Ning, Gang; Traktman, Paula; Duncan, Stephen A.

In: Journal of Biological Chemistry, Vol. 277, No. 47, 22.11.2002, p. 45347-45355.

Research output: Contribution to journalArticle

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