Petroleum biodegradation capacity of bacteria and fungi isolated from petroleum-contaminated soil

Charlotte Marchand, Marc St-Arnaud, William Hogland, Terrence Bell, Mohamed Hijri

Research output: Contribution to journalArticle

32 Citations (Scopus)

Abstract

We investigated the potential for petroleum hydrocarbon biodegradation by 95 bacterial and 160 fungal strains isolated from a former petrochemical plant. We tested whether soil origin, culture media type, and strain taxonomy influenced the degradation of added petroleum hydrocarbon compounds. Preliminary screening was based on two colorimetric tests using 2,6-dichlorophenolindophenol and p-iodonitrotetrazolium indicators, to assess microbial strain tolerance to crude oil. Top-performing strains in these screening assays were then assessed for their ability to mineralize a mixture of four polycyclic aromatic hydrocarbons (PAH) for 49 days, using GC-MS quantification. The aerobic activity of these candidate strains was also assessed by respirometry over the first 24 days of incubation. On average, PAH degradation by microbial isolates from soil that was lightly, moderately, and highly contaminated with petroleum was equally efficient, and the type of culture medium used did not significantly impact mean biodegradation. Phylogenetic affiliation had a strong and significant effect on PAH biodegradation. Fungal isolates belonging to the group Sordariomycetes, and bacterial isolates belonging to the groups Actinobacteria, Betaproteobacteria, and Gammaproteobacteria showed high potential for PAH degradation. Three of the strains tested by GC-MS, Rhodococcus sp., Trichoderma tomentosum, and Fusarium oxysporum, significantly degraded all four PAH compounds in the mixture compared to the control.

Original languageEnglish (US)
Pages (from-to)48-57
Number of pages10
JournalInternational Biodeterioration and Biodegradation
Volume116
DOIs
StatePublished - Jan 1 2017

Fingerprint

Polycyclic Aromatic Hydrocarbons
Petroleum
Polycyclic aromatic hydrocarbons
Biodegradation
Fungi
biodegradation
Bacteria
PAH
Soil
Crude oil
petroleum
fungus
Soils
bacterium
petroleum hydrocarbon
Hydrocarbons
Degradation
degradation
Culture Media
Screening

All Science Journal Classification (ASJC) codes

  • Microbiology
  • Biomaterials
  • Waste Management and Disposal

Cite this

Marchand, Charlotte ; St-Arnaud, Marc ; Hogland, William ; Bell, Terrence ; Hijri, Mohamed. / Petroleum biodegradation capacity of bacteria and fungi isolated from petroleum-contaminated soil. In: International Biodeterioration and Biodegradation. 2017 ; Vol. 116. pp. 48-57.
@article{37f84fc5142b4d25b4c67ec1c4cbb771,
title = "Petroleum biodegradation capacity of bacteria and fungi isolated from petroleum-contaminated soil",
abstract = "We investigated the potential for petroleum hydrocarbon biodegradation by 95 bacterial and 160 fungal strains isolated from a former petrochemical plant. We tested whether soil origin, culture media type, and strain taxonomy influenced the degradation of added petroleum hydrocarbon compounds. Preliminary screening was based on two colorimetric tests using 2,6-dichlorophenolindophenol and p-iodonitrotetrazolium indicators, to assess microbial strain tolerance to crude oil. Top-performing strains in these screening assays were then assessed for their ability to mineralize a mixture of four polycyclic aromatic hydrocarbons (PAH) for 49 days, using GC-MS quantification. The aerobic activity of these candidate strains was also assessed by respirometry over the first 24 days of incubation. On average, PAH degradation by microbial isolates from soil that was lightly, moderately, and highly contaminated with petroleum was equally efficient, and the type of culture medium used did not significantly impact mean biodegradation. Phylogenetic affiliation had a strong and significant effect on PAH biodegradation. Fungal isolates belonging to the group Sordariomycetes, and bacterial isolates belonging to the groups Actinobacteria, Betaproteobacteria, and Gammaproteobacteria showed high potential for PAH degradation. Three of the strains tested by GC-MS, Rhodococcus sp., Trichoderma tomentosum, and Fusarium oxysporum, significantly degraded all four PAH compounds in the mixture compared to the control.",
author = "Charlotte Marchand and Marc St-Arnaud and William Hogland and Terrence Bell and Mohamed Hijri",
year = "2017",
month = "1",
day = "1",
doi = "10.1016/j.ibiod.2016.09.030",
language = "English (US)",
volume = "116",
pages = "48--57",
journal = "International Biodeterioration and Biodegradation",
issn = "0964-8305",
publisher = "Elsevier Limited",

}

Petroleum biodegradation capacity of bacteria and fungi isolated from petroleum-contaminated soil. / Marchand, Charlotte; St-Arnaud, Marc; Hogland, William; Bell, Terrence; Hijri, Mohamed.

In: International Biodeterioration and Biodegradation, Vol. 116, 01.01.2017, p. 48-57.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Petroleum biodegradation capacity of bacteria and fungi isolated from petroleum-contaminated soil

AU - Marchand, Charlotte

AU - St-Arnaud, Marc

AU - Hogland, William

AU - Bell, Terrence

AU - Hijri, Mohamed

PY - 2017/1/1

Y1 - 2017/1/1

N2 - We investigated the potential for petroleum hydrocarbon biodegradation by 95 bacterial and 160 fungal strains isolated from a former petrochemical plant. We tested whether soil origin, culture media type, and strain taxonomy influenced the degradation of added petroleum hydrocarbon compounds. Preliminary screening was based on two colorimetric tests using 2,6-dichlorophenolindophenol and p-iodonitrotetrazolium indicators, to assess microbial strain tolerance to crude oil. Top-performing strains in these screening assays were then assessed for their ability to mineralize a mixture of four polycyclic aromatic hydrocarbons (PAH) for 49 days, using GC-MS quantification. The aerobic activity of these candidate strains was also assessed by respirometry over the first 24 days of incubation. On average, PAH degradation by microbial isolates from soil that was lightly, moderately, and highly contaminated with petroleum was equally efficient, and the type of culture medium used did not significantly impact mean biodegradation. Phylogenetic affiliation had a strong and significant effect on PAH biodegradation. Fungal isolates belonging to the group Sordariomycetes, and bacterial isolates belonging to the groups Actinobacteria, Betaproteobacteria, and Gammaproteobacteria showed high potential for PAH degradation. Three of the strains tested by GC-MS, Rhodococcus sp., Trichoderma tomentosum, and Fusarium oxysporum, significantly degraded all four PAH compounds in the mixture compared to the control.

AB - We investigated the potential for petroleum hydrocarbon biodegradation by 95 bacterial and 160 fungal strains isolated from a former petrochemical plant. We tested whether soil origin, culture media type, and strain taxonomy influenced the degradation of added petroleum hydrocarbon compounds. Preliminary screening was based on two colorimetric tests using 2,6-dichlorophenolindophenol and p-iodonitrotetrazolium indicators, to assess microbial strain tolerance to crude oil. Top-performing strains in these screening assays were then assessed for their ability to mineralize a mixture of four polycyclic aromatic hydrocarbons (PAH) for 49 days, using GC-MS quantification. The aerobic activity of these candidate strains was also assessed by respirometry over the first 24 days of incubation. On average, PAH degradation by microbial isolates from soil that was lightly, moderately, and highly contaminated with petroleum was equally efficient, and the type of culture medium used did not significantly impact mean biodegradation. Phylogenetic affiliation had a strong and significant effect on PAH biodegradation. Fungal isolates belonging to the group Sordariomycetes, and bacterial isolates belonging to the groups Actinobacteria, Betaproteobacteria, and Gammaproteobacteria showed high potential for PAH degradation. Three of the strains tested by GC-MS, Rhodococcus sp., Trichoderma tomentosum, and Fusarium oxysporum, significantly degraded all four PAH compounds in the mixture compared to the control.

UR - http://www.scopus.com/inward/record.url?scp=84991043173&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84991043173&partnerID=8YFLogxK

U2 - 10.1016/j.ibiod.2016.09.030

DO - 10.1016/j.ibiod.2016.09.030

M3 - Article

AN - SCOPUS:84991043173

VL - 116

SP - 48

EP - 57

JO - International Biodeterioration and Biodegradation

JF - International Biodeterioration and Biodegradation

SN - 0964-8305

ER -