Pharmacokinetics in rats and tissue distribution in mouse of berberrubine by UPLC-MS/MS

Xianqin Wang, Shuanghu Wang, Jianshe Ma, Tao Ye, Mengrou Lu, Miao Fan, Mingjie Deng, Lufeng Hu, Zhimou Gao

Research output: Contribution to journalArticle

52 Citations (Scopus)

Abstract

Berberrubine is an isoquinoline alkaloid isolated from Berberis vulgaris L, and it is readily derived from berberine. In this study, a sensitive and selective ultra performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) method for the determination of berberrubine in rat plasma and mouse tissue has been developed. Magnoflorine was employed as an internal standard (IS), and liquid-liquid extraction by ethyl acetate was used for sample preparation. Chromatographic separation was achieved on a UPLC BEH C18 column (2.1. mm. ×. 100. mm, 1.7. μm) with 0.1% formic acid and acetonitrile as the mobile phase with gradient elution. An electrospray ionization source was applied and operated in positive ion mode; multiple reactions monitoring (MRM) mode was used for quantification using target fragment ions m/. z 322.0. →. 307.0 for berberrubine and m/. z 342.8. →. 298.2 for IS. Calibration plots were linear in the range of 2-2000. ng/mL for berberrubine in rat plasma and mouse tissue. Mean recoveries of berberrubine in rat plasma ranged from 79.6% to 84.8%. Intra-day and inter-day precision were less than 11%. The accuracy ranged from 93.6% to 106.8%. The method has also been successfully applied in pharmacokinetics and tissue distribution study of berberrubine. The absolute bioavailability of berberrubine was determined to be 31.6%. The results also show that berberrubine is rapidly absorbed and widely distributed in various tissues. The level of berberrubine in liver is highest, and followed by kidney, spleen and heart. Furthermore, the concentration of berberrubine in various tissues could also be predicted by a BP-ANN model.

Original languageEnglish (US)
Pages (from-to)368-374
Number of pages7
JournalJournal of Pharmaceutical and Biomedical Analysis
Volume115
DOIs
StatePublished - Nov 1 2015

Fingerprint

Pharmacokinetics
Tissue Distribution
Rats
Tissue
formic acid
Plasmas
Berberis
berberrubine
Ions
Berberine
Electrospray ionization
Liquid-Liquid Extraction
Liquid chromatography
Liquids
Ion sources
Tandem Mass Spectrometry
Alkaloids
Liquid Chromatography
Liver
Calibration

All Science Journal Classification (ASJC) codes

  • Analytical Chemistry
  • Pharmaceutical Science
  • Drug Discovery
  • Spectroscopy
  • Clinical Biochemistry

Cite this

Wang, Xianqin ; Wang, Shuanghu ; Ma, Jianshe ; Ye, Tao ; Lu, Mengrou ; Fan, Miao ; Deng, Mingjie ; Hu, Lufeng ; Gao, Zhimou. / Pharmacokinetics in rats and tissue distribution in mouse of berberrubine by UPLC-MS/MS. In: Journal of Pharmaceutical and Biomedical Analysis. 2015 ; Vol. 115. pp. 368-374.
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title = "Pharmacokinetics in rats and tissue distribution in mouse of berberrubine by UPLC-MS/MS",
abstract = "Berberrubine is an isoquinoline alkaloid isolated from Berberis vulgaris L, and it is readily derived from berberine. In this study, a sensitive and selective ultra performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) method for the determination of berberrubine in rat plasma and mouse tissue has been developed. Magnoflorine was employed as an internal standard (IS), and liquid-liquid extraction by ethyl acetate was used for sample preparation. Chromatographic separation was achieved on a UPLC BEH C18 column (2.1. mm. ×. 100. mm, 1.7. μm) with 0.1{\%} formic acid and acetonitrile as the mobile phase with gradient elution. An electrospray ionization source was applied and operated in positive ion mode; multiple reactions monitoring (MRM) mode was used for quantification using target fragment ions m/. z 322.0. →. 307.0 for berberrubine and m/. z 342.8. →. 298.2 for IS. Calibration plots were linear in the range of 2-2000. ng/mL for berberrubine in rat plasma and mouse tissue. Mean recoveries of berberrubine in rat plasma ranged from 79.6{\%} to 84.8{\%}. Intra-day and inter-day precision were less than 11{\%}. The accuracy ranged from 93.6{\%} to 106.8{\%}. The method has also been successfully applied in pharmacokinetics and tissue distribution study of berberrubine. The absolute bioavailability of berberrubine was determined to be 31.6{\%}. The results also show that berberrubine is rapidly absorbed and widely distributed in various tissues. The level of berberrubine in liver is highest, and followed by kidney, spleen and heart. Furthermore, the concentration of berberrubine in various tissues could also be predicted by a BP-ANN model.",
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Pharmacokinetics in rats and tissue distribution in mouse of berberrubine by UPLC-MS/MS. / Wang, Xianqin; Wang, Shuanghu; Ma, Jianshe; Ye, Tao; Lu, Mengrou; Fan, Miao; Deng, Mingjie; Hu, Lufeng; Gao, Zhimou.

In: Journal of Pharmaceutical and Biomedical Analysis, Vol. 115, 01.11.2015, p. 368-374.

Research output: Contribution to journalArticle

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AU - Wang, Xianqin

AU - Wang, Shuanghu

AU - Ma, Jianshe

AU - Ye, Tao

AU - Lu, Mengrou

AU - Fan, Miao

AU - Deng, Mingjie

AU - Hu, Lufeng

AU - Gao, Zhimou

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