Pharmacological modulation of the pontine micturition center

B. S. Mallory, J. R. Roppolo, W. C. de Groat

Research output: Contribution to journalArticle

121 Citations (Scopus)

Abstract

Previous studies have indicated that an area of the rostral pontine tegmentum known as the 'pontine micturition center' (PMC) plays an essential role in the regulation of lower urinary tract function. The present pharmacologic experiments were conducted on either decerebrate unanesthetized or chloralose anesthetized cats to identify the location of the PMC and to examine the neurotransmitter mechanism controlling micturition. Microinjections of excitatory and inhibitory amino acids were made at stereotaxic coordinates P1 to P3, L2 to L3, H0 to H-4 where electrical stimulation with trains of pulses (2-30 V, 80-120 Hz and 50-300 ms train duration) elicited short latency (< 2 s) bladder contractions or voiding. Injections of L-glutamate (L-GLUT) (20-130 nmol) or DL-homocysteic acid (DLH) (20-100 nmol) into the region of the locus coeruleus or parabrachial nucleus elicited voiding as well as an increase in the frequency or amplitude of isovolumetric bladder contractions. In some anesthetized animals, L-GLUT and DLH also had mixed excitatory-inhibitory or pure inhibitory effects. Injections of muscimol (9-70 nmol) depressed rhythmic bladder activity, increased the bladder volume for inducing micturition or completely abolished the voiding induced by bladder filling. The inhibitory effects of muscimol were reversed by microinjections of bicuculine methiodide (BCMI) (3-22 nmol). Injections of BCMI (1-1.5 nmol) into untreated cats stimulated bladder activity and lowered the bladder volume for inducing micturition. It is concluded that: (1) neurons in the rostral pons are an essential component of the micturition reflex pathway, (2) several populations of neurons located in the region of the locus coeruleus complex and parabrachial nucleus contribute to the functions of the PMC, and (3) PMC neurons are under a tonic GABAergic inhibitory control which regulates the micturition threshold and in turn regulates bladder capacity.

Original languageEnglish (US)
Pages (from-to)310-320
Number of pages11
JournalBrain research
Volume546
Issue number2
DOIs
StatePublished - Apr 19 1991

Fingerprint

Urination
Urinary Bladder
Pharmacology
Muscimol
Locus Coeruleus
Microinjections
Neurons
Injections
Cats
Chloralose
Excitatory Amino Acids
Pons
Urinary Tract
Electric Stimulation
Neurotransmitter Agents
Reflex
Glutamic Acid

All Science Journal Classification (ASJC) codes

  • Neuroscience(all)
  • Molecular Biology
  • Clinical Neurology
  • Developmental Biology

Cite this

Mallory, B. S. ; Roppolo, J. R. ; de Groat, W. C. / Pharmacological modulation of the pontine micturition center. In: Brain research. 1991 ; Vol. 546, No. 2. pp. 310-320.
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Pharmacological modulation of the pontine micturition center. / Mallory, B. S.; Roppolo, J. R.; de Groat, W. C.

In: Brain research, Vol. 546, No. 2, 19.04.1991, p. 310-320.

Research output: Contribution to journalArticle

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