Phase separation as a possible means of nuclear compartmentalization

William M. Aumiller, Bradley W. Davis, Christine Dolan Keating

Research output: Chapter in Book/Report/Conference proceedingChapter

22 Citations (Scopus)

Abstract

The nucleus is perhaps the most familiar organelle within eukaryotic cells, serving as a compartment to house the genetic material. The nuclear volume is subdivided into a variety of functional and dynamic nuclear bodies not separated from the nucleoplasm by membranes. It has been hypothesized that aqueous phase separation brought about by macromolecular crowding may be in part responsible for these intranuclear compartments. This chapter discusses macromolecular solution chemistry with regard to several common types of phase separation in polymer solutions as well as to recent evidence that suggests that cytoplasmic and nuclear bodies may exist as liquid phases. We then examine the functional significance of phase separation and how it may serve as a means of compartmentalizing various nuclear activities, and describe recent studies that have used simple model systems to generate coexisting aqueous phase compartments, concentrate molecules within them, and perform localized biochemical reactions.

Original languageEnglish (US)
Title of host publicationInternational Review of Cell and Molecular Biology
PublisherElsevier Inc.
Pages109-149
Number of pages41
DOIs
StatePublished - Jan 1 2014

Publication series

NameInternational Review of Cell and Molecular Biology
Volume307
ISSN (Print)1937-6448

Fingerprint

Phase separation
Eukaryotic Cells
Organelles
Polymers
Polymer solutions
Membranes
Genes
Molecules
Liquids

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Aumiller, W. M., Davis, B. W., & Keating, C. D. (2014). Phase separation as a possible means of nuclear compartmentalization. In International Review of Cell and Molecular Biology (pp. 109-149). (International Review of Cell and Molecular Biology; Vol. 307). Elsevier Inc.. https://doi.org/10.1016/B978-0-12-800046-5.00005-9
Aumiller, William M. ; Davis, Bradley W. ; Keating, Christine Dolan. / Phase separation as a possible means of nuclear compartmentalization. International Review of Cell and Molecular Biology. Elsevier Inc., 2014. pp. 109-149 (International Review of Cell and Molecular Biology).
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Aumiller, WM, Davis, BW & Keating, CD 2014, Phase separation as a possible means of nuclear compartmentalization. in International Review of Cell and Molecular Biology. International Review of Cell and Molecular Biology, vol. 307, Elsevier Inc., pp. 109-149. https://doi.org/10.1016/B978-0-12-800046-5.00005-9

Phase separation as a possible means of nuclear compartmentalization. / Aumiller, William M.; Davis, Bradley W.; Keating, Christine Dolan.

International Review of Cell and Molecular Biology. Elsevier Inc., 2014. p. 109-149 (International Review of Cell and Molecular Biology; Vol. 307).

Research output: Chapter in Book/Report/Conference proceedingChapter

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Aumiller WM, Davis BW, Keating CD. Phase separation as a possible means of nuclear compartmentalization. In International Review of Cell and Molecular Biology. Elsevier Inc. 2014. p. 109-149. (International Review of Cell and Molecular Biology). https://doi.org/10.1016/B978-0-12-800046-5.00005-9