Phloem ultrastructure and pressure flow: Sieve-element-occlusion-related agglomerations do not affect translocation

Daniel R. Froelich, Daniel L. Mullendore, Kåre H. Jensen, Tim J. Ross-Elliott, James A. Anstead, Gary A. Thompson, Hélène C. Pélissier, Michael Knoblauch

Research output: Contribution to journalArticlepeer-review

110 Scopus citations

Abstract

Since the first ultrastructural investigations of sieve tubes in the early 1960s, their structure has been a matter of debate. Because sieve tube structure defines frictional interactions in the tube system, the presence of P protein obstructions shown in many transmission electron micrographs led to a discussion about the mode of phloem transport. At present, it is generally agreed that P protein agglomerations are preparation artifacts due to injury, the lumen of sieve tubes is free of obstructions, and phloem flow is driven by an osmotically generated pressure differential according to Münch's classical hypothesis. Here, we show that the phloem contains a distinctive network of protein filaments. Stable transgenic lines expressing Arabidopsis thaliana Sieve-Element-Occlusion-Related1 (SEOR1)-yellow fluorescent protein fusions show that At SEOR1 meshworks at the margins and clots in the lumen are a general feature of living sieve tubes. Live imaging of phloem flow and flow velocity measurements in individual tubes indicate that At SEOR1 agglomerations do not markedly affect or alter flow. A transmission electron microscopy preparation protocol has been generated showing sieve tube ultrastructure of unprecedented quality. A reconstruction of sieve tube ultrastructure served as basis for tube resistance calculations. The impact of agglomerations on phloem flow is discussed.

Original languageEnglish (US)
Pages (from-to)4428-4445
Number of pages18
JournalPlant Cell
Volume23
Issue number12
DOIs
StatePublished - Dec 2011

All Science Journal Classification (ASJC) codes

  • Plant Science
  • Cell Biology

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