Phospholipase Cγ1 controls surface expression of TRPC3 through an intermolecular PH domain

Damian B. Van Rossum, Randen L. Patterson, Sumit Sharma, Roxanne K. Barrow, Michael Kornberg, Donald L. Gill, Solomon H. Snyder

Research output: Contribution to journalArticle

159 Citations (Scopus)

Abstract

Many ion channels are regulated by lipids, but prominent motifs for lipid binding have not been identified in most ion channels. Recently, we reported that phospholipase Cγ1 (PLC-γ1) binds to and regulates TRPC3 channels, components of agonist-induced Ca2+ entry into cells. This interaction requires a domain in PLC-γ1 that includes a partial pleckstrin homology (PH) domain-a consensus lipid-binding and protein-binding sequence. We have developed a gestalt algorithm to detect hitherto 'invisible' PH and PH-like domains, and now report that the partial PH domain of PLC-γ1 interacts with a complementary partial PH-like domain in TRPC3 to elicit lipid binding and cell-surface expression of TRPC3. Our findings imply a far greater abundance of PH domains than previously appreciated, and suggest that intermolecular PH-like domains represent a widespread signalling mode.

Original languageEnglish (US)
Pages (from-to)99-104
Number of pages6
JournalNature
Volume434
Issue number7029
DOIs
StatePublished - Mar 3 2005

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Lipids
Ion Channels
Protein Binding
Pleckstrin Homology Domains
phospholipase C1
Carrier Proteins
TRPC3 cation channel
platelet protein P47

All Science Journal Classification (ASJC) codes

  • General

Cite this

Van Rossum, D. B., Patterson, R. L., Sharma, S., Barrow, R. K., Kornberg, M., Gill, D. L., & Snyder, S. H. (2005). Phospholipase Cγ1 controls surface expression of TRPC3 through an intermolecular PH domain. Nature, 434(7029), 99-104. https://doi.org/10.1038/nature03340
Van Rossum, Damian B. ; Patterson, Randen L. ; Sharma, Sumit ; Barrow, Roxanne K. ; Kornberg, Michael ; Gill, Donald L. ; Snyder, Solomon H. / Phospholipase Cγ1 controls surface expression of TRPC3 through an intermolecular PH domain. In: Nature. 2005 ; Vol. 434, No. 7029. pp. 99-104.
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Van Rossum, DB, Patterson, RL, Sharma, S, Barrow, RK, Kornberg, M, Gill, DL & Snyder, SH 2005, 'Phospholipase Cγ1 controls surface expression of TRPC3 through an intermolecular PH domain', Nature, vol. 434, no. 7029, pp. 99-104. https://doi.org/10.1038/nature03340

Phospholipase Cγ1 controls surface expression of TRPC3 through an intermolecular PH domain. / Van Rossum, Damian B.; Patterson, Randen L.; Sharma, Sumit; Barrow, Roxanne K.; Kornberg, Michael; Gill, Donald L.; Snyder, Solomon H.

In: Nature, Vol. 434, No. 7029, 03.03.2005, p. 99-104.

Research output: Contribution to journalArticle

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