Phosphorylation of insulin-like growth factor binding protein-1 in patients with insulin-dependent diabetes mellitus and severe trauma

Robert A. Frost, Abdullah Bereket, Thomas A. Wilson, Margaret M. Wojna, Charles H. Lang, Marie C. Gelato

Research output: Contribution to journalArticle

33 Citations (Scopus)

Abstract

We have determined the level of phosphorylated insulin-like growth factor binding protein-1 (pIGFBP-1) in serum during two catabolic states: diabetes mellitus and trauma. Human sera were incubated with [125I]IGF-I for 2 h followed by non-denaturing PAGE. [125I]IGF-I/IGFBP-1 complexes from serum co-migrated with a pure p4IGFBP-1 standard. Complex formation was specifically inhibited by unlabeled IGF-I. The migration of IGF-I/pIGFBP-1 complexes was retarded by IGFBP-1 antibodies, but not by antibodies against IGFBP-2 or IGFBP-3. Sera from three severely traumatized patients had up to 12-fold more pIGFBP-1 than sera from age-matched controls. The level of pIGFBP-1 was reduced in all three patients upon hospital discharge. Sera from three patients with insulin dependent diabetes mellitus (IDDM) and severe ketoacidosis (DKA) had more pIGFBP-1 than controls. Administration of insulin to DKA patients lowered the level of pIGFBP-1. The present study shows that IGFBP-1 exists as a free, high affinity, phosphorylated form in vivo during two catabolic states.

Original languageEnglish (US)
Pages (from-to)1533-1535
Number of pages3
JournalJournal of Clinical Endocrinology and Metabolism
Volume78
Issue number6
DOIs
StatePublished - Jan 1 1994

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Insulin-Like Growth Factor Binding Protein 1
Phosphorylation
Medical problems
Type 1 Diabetes Mellitus
Insulin
Wounds and Injuries
Insulin-Like Growth Factor I
Serum
Insulin-Like Growth Factor Binding Protein 2
Native Polyacrylamide Gel Electrophoresis
Insulin-Like Growth Factor Binding Protein 3
Ketosis
Insulin-Like Growth Factor II
Antibodies
Diabetes Mellitus

All Science Journal Classification (ASJC) codes

  • Endocrinology, Diabetes and Metabolism
  • Biochemistry
  • Endocrinology
  • Clinical Biochemistry
  • Biochemistry, medical

Cite this

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title = "Phosphorylation of insulin-like growth factor binding protein-1 in patients with insulin-dependent diabetes mellitus and severe trauma",
abstract = "We have determined the level of phosphorylated insulin-like growth factor binding protein-1 (pIGFBP-1) in serum during two catabolic states: diabetes mellitus and trauma. Human sera were incubated with [125I]IGF-I for 2 h followed by non-denaturing PAGE. [125I]IGF-I/IGFBP-1 complexes from serum co-migrated with a pure p4IGFBP-1 standard. Complex formation was specifically inhibited by unlabeled IGF-I. The migration of IGF-I/pIGFBP-1 complexes was retarded by IGFBP-1 antibodies, but not by antibodies against IGFBP-2 or IGFBP-3. Sera from three severely traumatized patients had up to 12-fold more pIGFBP-1 than sera from age-matched controls. The level of pIGFBP-1 was reduced in all three patients upon hospital discharge. Sera from three patients with insulin dependent diabetes mellitus (IDDM) and severe ketoacidosis (DKA) had more pIGFBP-1 than controls. Administration of insulin to DKA patients lowered the level of pIGFBP-1. The present study shows that IGFBP-1 exists as a free, high affinity, phosphorylated form in vivo during two catabolic states.",
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Phosphorylation of insulin-like growth factor binding protein-1 in patients with insulin-dependent diabetes mellitus and severe trauma. / Frost, Robert A.; Bereket, Abdullah; Wilson, Thomas A.; Wojna, Margaret M.; Lang, Charles H.; Gelato, Marie C.

In: Journal of Clinical Endocrinology and Metabolism, Vol. 78, No. 6, 01.01.1994, p. 1533-1535.

Research output: Contribution to journalArticle

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AU - Lang, Charles H.

AU - Gelato, Marie C.

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