Photoaffinity labelling of a nuclear matrix nucleoside triphosphatase and its modulation in the acute-phase response

Gary A. Clawson, Jane Button, Edward A. Smuckler

Research output: Contribution to journalArticlepeer-review

6 Scopus citations

Abstract

Photoaffinity labelling has been used to identify the major nuclear matrix nucleoside triphosphatase (NTPase) as a 46 kD polypeptide, which appears to represent the same polypeptide photolabelled in nuclear envelope. Nuclear matrix NTPase and its photolabelling were selectively decreased in the acute phase response of rat liver, which also encompasses decreases (30%) in RNA transport in vitro and nuclear envelope NTPase. These results, and quantitative considerations suggest that the NTPase correlatively linked to RNA transport is not solubilized by detergents; it appears to represent a nuclear matrix component.

Original languageEnglish (US)
Pages (from-to)171-175
Number of pages5
JournalExperimental Cell Research
Volume159
Issue number1
DOIs
StatePublished - Jul 1985

All Science Journal Classification (ASJC) codes

  • Cell Biology

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