Photoautotrophic hydrogen production by eukaryotic microalgae under aerobic conditions

Jae Hoon Hwang, Hyun Chul Kim, Jeong A. Choi, R. A.I. Abou-Shanab, Brian A. Dempsey, John Ragan, Jung Rae Kim, Hocheol Song, In Hyun Nam, Su Nam Kim, Woojung Lee, Donghee Park, Yongje Kim, Jaeyoung Choi, Min Kyu Ji, Woosik Jung, Byong Hun Jeon

Research output: Contribution to journalArticle

47 Citations (Scopus)

Abstract

Eukaryotic algae and cyanobacteria produce hydrogen under anaerobic and limited aerobic conditions. Here we show that novel microalgal strains (Chlorella vulgaris YSL01 and YSL16) upregulate the expression of the hydrogenase gene (HYDA) and simultaneously produce hydrogen through photosynthesis, using CO 2 as the sole source of carbon under aerobic conditions with continuous illumination. We employ dissolved oxygen regimes that represent natural aquatic conditions for microalgae. The experimental expression of HYDA and the specific activity of hydrogenase demonstrate that C. vulgaris YSL01 and YSL16 enzymatically produce hydrogen, even under atmospheric conditions, which was previously considered infeasible. Photoautotrophic H 2 production has important implications for assessing ecological and algae-based photolysis.

Original languageEnglish (US)
Article number3234
JournalNature communications
Volume5
DOIs
StatePublished - Feb 4 2014

Fingerprint

Microalgae
Hydrogenase
hydrogen production
Hydrogen production
Chlorella vulgaris
Hydrogen
algae
Algae
genes
hydrogen
Genes
Chlorella
Gene Expression
photosynthesis
Photosynthesis
Photolysis
Cyanobacteria
Carbon Monoxide
Dissolved oxygen
meteorology

All Science Journal Classification (ASJC) codes

  • Chemistry(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Physics and Astronomy(all)

Cite this

Hwang, J. H., Kim, H. C., Choi, J. A., Abou-Shanab, R. A. I., Dempsey, B. A., Ragan, J., ... Jeon, B. H. (2014). Photoautotrophic hydrogen production by eukaryotic microalgae under aerobic conditions. Nature communications, 5, [3234]. https://doi.org/10.1038/ncomms4234
Hwang, Jae Hoon ; Kim, Hyun Chul ; Choi, Jeong A. ; Abou-Shanab, R. A.I. ; Dempsey, Brian A. ; Ragan, John ; Kim, Jung Rae ; Song, Hocheol ; Nam, In Hyun ; Kim, Su Nam ; Lee, Woojung ; Park, Donghee ; Kim, Yongje ; Choi, Jaeyoung ; Ji, Min Kyu ; Jung, Woosik ; Jeon, Byong Hun. / Photoautotrophic hydrogen production by eukaryotic microalgae under aerobic conditions. In: Nature communications. 2014 ; Vol. 5.
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Hwang, JH, Kim, HC, Choi, JA, Abou-Shanab, RAI, Dempsey, BA, Ragan, J, Kim, JR, Song, H, Nam, IH, Kim, SN, Lee, W, Park, D, Kim, Y, Choi, J, Ji, MK, Jung, W & Jeon, BH 2014, 'Photoautotrophic hydrogen production by eukaryotic microalgae under aerobic conditions', Nature communications, vol. 5, 3234. https://doi.org/10.1038/ncomms4234

Photoautotrophic hydrogen production by eukaryotic microalgae under aerobic conditions. / Hwang, Jae Hoon; Kim, Hyun Chul; Choi, Jeong A.; Abou-Shanab, R. A.I.; Dempsey, Brian A.; Ragan, John; Kim, Jung Rae; Song, Hocheol; Nam, In Hyun; Kim, Su Nam; Lee, Woojung; Park, Donghee; Kim, Yongje; Choi, Jaeyoung; Ji, Min Kyu; Jung, Woosik; Jeon, Byong Hun.

In: Nature communications, Vol. 5, 3234, 04.02.2014.

Research output: Contribution to journalArticle

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