Phylogenetic diversity of insecticolous fusaria inferred from multilocus DNA sequence data and their molecular identification via FUSARIUM-ID and Fusarium MLST

Kerry O'Donnell, Richard A. Humber, David Michael Geiser, Seogchan Kang, Bongsoo Park, Vincent A.R.G. Robert, Pedro W. Crous, Peter R. Johnston, Takayuki Aoki, Alejandro P. Rooney, Stephen A. Rehner

Research output: Contribution to journalArticle

79 Citations (Scopus)

Abstract

We constructed several multilocus DNA sequence datasets to assess the phylogenetic diversity of insecticolous fusaria, especially focusing on those housed at the Agricultural Research Service Collection of Entomopathogenic Fungi (ARSEF), and to aid molecular identifications of unknowns via the FUSARIUM-ID and Fusarium MLST online databases and analysis packages. Analyses of a 190-taxon, twolocus dataset, which included 159 isolates from insects, indicated that: (i) insect-associated fusaria were nested within 10 species complexes spanning the phylogenetic breadth of Fusarium, (ii) novel, putatively unnamed insecticolous species were nested within 8/10 species complexes and (iii) Latin binomials could be applied with confidence to only 18/58 phylogenetically distinct fusaria associated with pest insects. Phylogenetic analyses of an 82-taxon, three-locus dataset nearly fully resolved evolutionary relationships among the 10 clades containing insecticolous fusaria. Multilocus typing of isolates within four species complexes identified surprisingly high genetic diversity in that 63/65 of the fusaria typed represented newly discovered haplotypes. The DNA sequence data, together with corrected ABI sequence chromatograms and alignments, have been uploaded to the following websites dedicated to identifying fusaria: FUSARIUM-ID (http://isolate.fusariumdb.org) at Pennsylvania State University's Department of Plant Pathology and Fusarium MLST (http://www.cbs.knaw.nl/fusarium) at the Centraalbureau voor Schimmelcultures (CBS-KNAW) Fungal Biodiversity Center.

Original languageEnglish (US)
Pages (from-to)427-445
Number of pages19
JournalMycologia
Volume104
Issue number2
DOIs
StatePublished - Mar 1 2012

Fingerprint

Fusarium
species complex
insect
phylogenetics
Insects
nucleotide sequences
DNA
phylogeny
agricultural research
Plant Pathology
pathology
insects
Agricultural Research Service
Sequence Alignment
plant pathology
Biodiversity
entomopathogenic fungi
fungus
biodiversity
insect pests

All Science Journal Classification (ASJC) codes

  • Ecology, Evolution, Behavior and Systematics
  • Physiology
  • Molecular Biology
  • Genetics
  • Cell Biology

Cite this

O'Donnell, Kerry ; Humber, Richard A. ; Geiser, David Michael ; Kang, Seogchan ; Park, Bongsoo ; Robert, Vincent A.R.G. ; Crous, Pedro W. ; Johnston, Peter R. ; Aoki, Takayuki ; Rooney, Alejandro P. ; Rehner, Stephen A. / Phylogenetic diversity of insecticolous fusaria inferred from multilocus DNA sequence data and their molecular identification via FUSARIUM-ID and Fusarium MLST. In: Mycologia. 2012 ; Vol. 104, No. 2. pp. 427-445.
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abstract = "We constructed several multilocus DNA sequence datasets to assess the phylogenetic diversity of insecticolous fusaria, especially focusing on those housed at the Agricultural Research Service Collection of Entomopathogenic Fungi (ARSEF), and to aid molecular identifications of unknowns via the FUSARIUM-ID and Fusarium MLST online databases and analysis packages. Analyses of a 190-taxon, twolocus dataset, which included 159 isolates from insects, indicated that: (i) insect-associated fusaria were nested within 10 species complexes spanning the phylogenetic breadth of Fusarium, (ii) novel, putatively unnamed insecticolous species were nested within 8/10 species complexes and (iii) Latin binomials could be applied with confidence to only 18/58 phylogenetically distinct fusaria associated with pest insects. Phylogenetic analyses of an 82-taxon, three-locus dataset nearly fully resolved evolutionary relationships among the 10 clades containing insecticolous fusaria. Multilocus typing of isolates within four species complexes identified surprisingly high genetic diversity in that 63/65 of the fusaria typed represented newly discovered haplotypes. The DNA sequence data, together with corrected ABI sequence chromatograms and alignments, have been uploaded to the following websites dedicated to identifying fusaria: FUSARIUM-ID (http://isolate.fusariumdb.org) at Pennsylvania State University's Department of Plant Pathology and Fusarium MLST (http://www.cbs.knaw.nl/fusarium) at the Centraalbureau voor Schimmelcultures (CBS-KNAW) Fungal Biodiversity Center.",
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O'Donnell, K, Humber, RA, Geiser, DM, Kang, S, Park, B, Robert, VARG, Crous, PW, Johnston, PR, Aoki, T, Rooney, AP & Rehner, SA 2012, 'Phylogenetic diversity of insecticolous fusaria inferred from multilocus DNA sequence data and their molecular identification via FUSARIUM-ID and Fusarium MLST', Mycologia, vol. 104, no. 2, pp. 427-445. https://doi.org/10.3852/11-179

Phylogenetic diversity of insecticolous fusaria inferred from multilocus DNA sequence data and their molecular identification via FUSARIUM-ID and Fusarium MLST. / O'Donnell, Kerry; Humber, Richard A.; Geiser, David Michael; Kang, Seogchan; Park, Bongsoo; Robert, Vincent A.R.G.; Crous, Pedro W.; Johnston, Peter R.; Aoki, Takayuki; Rooney, Alejandro P.; Rehner, Stephen A.

In: Mycologia, Vol. 104, No. 2, 01.03.2012, p. 427-445.

Research output: Contribution to journalArticle

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AU - Humber, Richard A.

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AU - Kang, Seogchan

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AU - Robert, Vincent A.R.G.

AU - Crous, Pedro W.

AU - Johnston, Peter R.

AU - Aoki, Takayuki

AU - Rooney, Alejandro P.

AU - Rehner, Stephen A.

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