TY - JOUR
T1 - Phylogenetic relationships of Staphylococcus aureus from bovine mastitis based on coagulase gene polymorphism
AU - Su, C.
AU - Kanevsky, I.
AU - Jayarao, B. M.
AU - Sordillo, L. M.
N1 - Funding Information:
This research was supported in part by agricultural research funds administered by the Pennsylvania Department of Agriculture (ME445126) and the USDA-BARD Grants Program (US-2648-95). We appreciate Drs. B. Poutrel, J.W. Yoon and O. Skardova for providing S. aureus isolates from France, Korea and the Czech Republic, respectively.
PY - 2000/1
Y1 - 2000/1
N2 - Coagulase gene restriction fragment length polymorphism (RFLP) patterns were analyzed to determine the phylogenetic relationship among isolates of Staphylococcus aureus from the Czech Republic (n=27), France (n=48), Korea (n=115) and the United States (n=278). A total of 468 isolates of S. aureus were subtyped into 41 coagulase genotypes. Cluster analysis placed the 41 types into nine clusters. Eighteen API Staph profiles were determined for 102 S. aureus isolates representing 1 to 4 isolates of each coagulase type. The results of the study suggest that based on coagulase gene RFLP analysis, several genetic variants of S. aureus are prevalent. Comparison of coagulase and API Staph profiles indicated that the two identification system were independent of each other.
AB - Coagulase gene restriction fragment length polymorphism (RFLP) patterns were analyzed to determine the phylogenetic relationship among isolates of Staphylococcus aureus from the Czech Republic (n=27), France (n=48), Korea (n=115) and the United States (n=278). A total of 468 isolates of S. aureus were subtyped into 41 coagulase genotypes. Cluster analysis placed the 41 types into nine clusters. Eighteen API Staph profiles were determined for 102 S. aureus isolates representing 1 to 4 isolates of each coagulase type. The results of the study suggest that based on coagulase gene RFLP analysis, several genetic variants of S. aureus are prevalent. Comparison of coagulase and API Staph profiles indicated that the two identification system were independent of each other.
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U2 - 10.1016/S0378-1135(99)00139-X
DO - 10.1016/S0378-1135(99)00139-X
M3 - Article
C2 - 10665533
AN - SCOPUS:0033986481
VL - 71
SP - 53
EP - 58
JO - Veterinary Microbiology
JF - Veterinary Microbiology
SN - 0378-1135
IS - 1-2
ER -