Phylogeny of the phototrophic rhizobium strain BTAi1 by polymerase chain reaction-based sequencing of a 16S rRNA gene segment

J. P.W. Young, H. L. Downer, Bertrand D. Eardly

Research output: Contribution to journalArticle

296 Citations (Scopus)

Abstract

A 260-bp segment of the DNA that encodes 16S rRNA, corresponding to positions 44 to 337 in the Escherichia coli sequence, was amplified by the polymerase chain reaction and sequenced from each of 13 bacteria (rhizobia and purple phototrophs) in the alpha subdivision of the class Proteobacteria. The phylogenetic tree calculated from differences in the sequenced segment conforms well to our expectations based on other previously published data. The sequence from BTAi1 (a recently described phototrophic symbiont of the legume Aeschynomene) and that from the free-living phototroph Rhodopseudomonas palustris both fall within the range of variation found among strains of the soybean symbiont Bradyhizobium japonicum. This suggests that it would be appropriate to include all of these organisms in a single genus.

Original languageEnglish (US)
Pages (from-to)2271-2277
Number of pages7
JournalJournal of Bacteriology
Volume173
Issue number7
DOIs
StatePublished - Jan 1 1991

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Proteobacteria
Rhizobium
Phylogeny
rRNA Genes
Rhodopseudomonas
Polymerase Chain Reaction
Soybeans
Fabaceae
Escherichia coli
DNA

All Science Journal Classification (ASJC) codes

  • Microbiology
  • Molecular Biology

Cite this

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Phylogeny of the phototrophic rhizobium strain BTAi1 by polymerase chain reaction-based sequencing of a 16S rRNA gene segment. / Young, J. P.W.; Downer, H. L.; Eardly, Bertrand D.

In: Journal of Bacteriology, Vol. 173, No. 7, 01.01.1991, p. 2271-2277.

Research output: Contribution to journalArticle

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