Physicochemical code for quinary protein interactions in Escherichia coli

Xin Mu, Seongil Choi, Lisa Lang, David Mowray, Nikolay Dokholyan, Jens Danielsson, Mikael Oliveberg

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

How proteins sense and navigate the cellular interior to find their functional partners remains poorly understood. An intriguing aspect of this search is that it relies on diffusive encounters with the crowded cellular background, made up of protein surfaces that are largely nonconserved. The question is then if/how this protein search is amenable to selection and biological control. To shed light on this issue, we examined the motions of three evolutionary divergent proteins in the Escherichia coli cytoplasm by in-cell NMR. The results show that the diffusive in-cell motions, after all, follow simplistic physicalchemical rules: The proteins reveal a common dependence on (i) net charge density, (ii) surface hydrophobicity, and (iii) the electric dipole moment. The bacterial protein is here biased to move relatively freely in the bacterial interior, whereas the human counterparts more easily stick. Even so, the in-cell motions respond predictably to surface mutation, allowing us to tune and intermix the protein's behavior at will. The findings show how evolution can swiftly optimize the diffuse background of protein encounter complexes by just single-point mutations, and provide a rational framework for adjusting the cytoplasmic motions of individual proteins, e.g., for rescuing poor in-cell NMR signals and for optimizing protein therapeutics.

Original languageEnglish (US)
Pages (from-to)E4556-E4563
JournalProceedings of the National Academy of Sciences of the United States of America
Volume114
Issue number23
DOIs
StatePublished - Jun 6 2017

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Escherichia coli
Proteins
Bacterial Proteins
Escherichia coli Proteins
Hydrophobic and Hydrophilic Interactions
Point Mutation
Membrane Proteins
Cytoplasm
Mutation
Therapeutics

All Science Journal Classification (ASJC) codes

  • General

Cite this

Mu, Xin ; Choi, Seongil ; Lang, Lisa ; Mowray, David ; Dokholyan, Nikolay ; Danielsson, Jens ; Oliveberg, Mikael. / Physicochemical code for quinary protein interactions in Escherichia coli. In: Proceedings of the National Academy of Sciences of the United States of America. 2017 ; Vol. 114, No. 23. pp. E4556-E4563.
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Physicochemical code for quinary protein interactions in Escherichia coli. / Mu, Xin; Choi, Seongil; Lang, Lisa; Mowray, David; Dokholyan, Nikolay; Danielsson, Jens; Oliveberg, Mikael.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 114, No. 23, 06.06.2017, p. E4556-E4563.

Research output: Contribution to journalArticle

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AU - Oliveberg, Mikael

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