Physiological function of Rac Prophage during biofilm formation and regulation of Rac excision in Escherichia coli K-12

Xiaoxiao Liu, Yangmei Li, Yunxue Guo, Zhenshun Zeng, Baiyuan Li, Thomas K. Wood, Xingsheng Cai, Xiaoxue Wang

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Rac or rac-like prophage harbors many genes with important physiological functions, while it remains excision-proficient in several bacterial strains including Escherichia coli, Salmonella spp. and Shigella spp. Here, we found that rac excision is induced during biofilm formation, and the isogenic stain without rac is more motile and forms more biofilms in nutrient-rich medium at early stages in E. coli K-12. Additionally, the presence of rac genes increases cell lysis during biofilm development. In most E. coli strains, rac is integrated into the ttcA gene which encodes a tRNA-thioltransferase. Rac excision in E. coli K-12 leads to a functional change of TtcA, which results in reduced fitness in the presence of carbenicillin. Additionally, we demonstrate that YdaQ (renamed as XisR) is the excisionase of rac in E. coli K-12, and that rac excision is induced by the stationary sigma factor RpoS through inducing xisR expression. Taken together, our results reveal that upon rac integration, not only are new genes introduced into the host, but also there is a functional change in a host enzyme. Hence, rac excision is tightly regulated by host factors to control its stability in the host genome under different stress conditions.

Original languageEnglish (US)
Article number16074
JournalScientific reports
Volume5
DOIs
StatePublished - Nov 4 2015

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Prophages
Biofilms
Escherichia coli
Genes
Glutaredoxins
Carbenicillin
Sigma Factor
Shigella
Transfer RNA
Salmonella
Coloring Agents
Genome
Food
Enzymes

All Science Journal Classification (ASJC) codes

  • General

Cite this

Liu, Xiaoxiao ; Li, Yangmei ; Guo, Yunxue ; Zeng, Zhenshun ; Li, Baiyuan ; Wood, Thomas K. ; Cai, Xingsheng ; Wang, Xiaoxue. / Physiological function of Rac Prophage during biofilm formation and regulation of Rac excision in Escherichia coli K-12. In: Scientific reports. 2015 ; Vol. 5.
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abstract = "Rac or rac-like prophage harbors many genes with important physiological functions, while it remains excision-proficient in several bacterial strains including Escherichia coli, Salmonella spp. and Shigella spp. Here, we found that rac excision is induced during biofilm formation, and the isogenic stain without rac is more motile and forms more biofilms in nutrient-rich medium at early stages in E. coli K-12. Additionally, the presence of rac genes increases cell lysis during biofilm development. In most E. coli strains, rac is integrated into the ttcA gene which encodes a tRNA-thioltransferase. Rac excision in E. coli K-12 leads to a functional change of TtcA, which results in reduced fitness in the presence of carbenicillin. Additionally, we demonstrate that YdaQ (renamed as XisR) is the excisionase of rac in E. coli K-12, and that rac excision is induced by the stationary sigma factor RpoS through inducing xisR expression. Taken together, our results reveal that upon rac integration, not only are new genes introduced into the host, but also there is a functional change in a host enzyme. Hence, rac excision is tightly regulated by host factors to control its stability in the host genome under different stress conditions.",
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Physiological function of Rac Prophage during biofilm formation and regulation of Rac excision in Escherichia coli K-12. / Liu, Xiaoxiao; Li, Yangmei; Guo, Yunxue; Zeng, Zhenshun; Li, Baiyuan; Wood, Thomas K.; Cai, Xingsheng; Wang, Xiaoxue.

In: Scientific reports, Vol. 5, 16074, 04.11.2015.

Research output: Contribution to journalArticle

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