Picosecond Time-Resolved Fluorescence from Detergent-Free Photosystem I Particles

Bruce P. Wittmershaus, Donald S. Berns, Cinnia Huang

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

Picosecond time-resolved fluorescence measurements have been taken on a detergent-free P700-enriched complex at room temperature isolated from the blue-green alga Phormidium luridum with a chlorophyll a to reaction center ratio of 100. Emission at greater than 665 nm is characterized by two exponential-decay components. A fast component, which dominates the initial decay with an average lifetime of 16 ps and 87% amplitude, is attributed to excitations in the core antenna chlorophyll-proteins, which are rapidly trapped by the primary electron donor, P700. A second component, with an average lifetime of 106 ps and 13% amplitude, is attributed to the peripheral antenna proteins. For 532-nm, 30-ps pulse excitation the results are virtually independent of fluence in the range of 2 × 1012 to 4 × 1016 photons/cm2 and the oxidation state of P700. Addition of sodium dodecyl sulfate to 0.1% causes the second component's lifetime to increase by an average of a factor of 2.5. Only minor changes are observed in the first component's lifetime and the relative amplitudes of the two components. Two fractions isolated from the detergent-treated samples have also been examined. Our results indicate that excitation energy transfer within photosystem I is very efficient and that the excitation kinetics of the antennae may be limited by the trapping rate of P700 or strongly affected by the heterogeneity of the antennae.

Original languageEnglish (US)
Pages (from-to)829-836
Number of pages8
JournalBiophysical journal
Volume52
Issue number5
DOIs
StatePublished - Jan 1 1987

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Photosystem I Protein Complex
Detergents
Fluorescence
Energy Transfer
Cyanobacteria
Chlorophyll
Photons
Sodium Dodecyl Sulfate
Proteins
Electrons
Temperature
chlorophyll a

All Science Journal Classification (ASJC) codes

  • Biophysics

Cite this

Wittmershaus, Bruce P. ; Berns, Donald S. ; Huang, Cinnia. / Picosecond Time-Resolved Fluorescence from Detergent-Free Photosystem I Particles. In: Biophysical journal. 1987 ; Vol. 52, No. 5. pp. 829-836.
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abstract = "Picosecond time-resolved fluorescence measurements have been taken on a detergent-free P700-enriched complex at room temperature isolated from the blue-green alga Phormidium luridum with a chlorophyll a to reaction center ratio of 100. Emission at greater than 665 nm is characterized by two exponential-decay components. A fast component, which dominates the initial decay with an average lifetime of 16 ps and 87{\%} amplitude, is attributed to excitations in the core antenna chlorophyll-proteins, which are rapidly trapped by the primary electron donor, P700. A second component, with an average lifetime of 106 ps and 13{\%} amplitude, is attributed to the peripheral antenna proteins. For 532-nm, 30-ps pulse excitation the results are virtually independent of fluence in the range of 2 × 1012 to 4 × 1016 photons/cm2 and the oxidation state of P700. Addition of sodium dodecyl sulfate to 0.1{\%} causes the second component's lifetime to increase by an average of a factor of 2.5. Only minor changes are observed in the first component's lifetime and the relative amplitudes of the two components. Two fractions isolated from the detergent-treated samples have also been examined. Our results indicate that excitation energy transfer within photosystem I is very efficient and that the excitation kinetics of the antennae may be limited by the trapping rate of P700 or strongly affected by the heterogeneity of the antennae.",
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Picosecond Time-Resolved Fluorescence from Detergent-Free Photosystem I Particles. / Wittmershaus, Bruce P.; Berns, Donald S.; Huang, Cinnia.

In: Biophysical journal, Vol. 52, No. 5, 01.01.1987, p. 829-836.

Research output: Contribution to journalArticle

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AU - Huang, Cinnia

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N2 - Picosecond time-resolved fluorescence measurements have been taken on a detergent-free P700-enriched complex at room temperature isolated from the blue-green alga Phormidium luridum with a chlorophyll a to reaction center ratio of 100. Emission at greater than 665 nm is characterized by two exponential-decay components. A fast component, which dominates the initial decay with an average lifetime of 16 ps and 87% amplitude, is attributed to excitations in the core antenna chlorophyll-proteins, which are rapidly trapped by the primary electron donor, P700. A second component, with an average lifetime of 106 ps and 13% amplitude, is attributed to the peripheral antenna proteins. For 532-nm, 30-ps pulse excitation the results are virtually independent of fluence in the range of 2 × 1012 to 4 × 1016 photons/cm2 and the oxidation state of P700. Addition of sodium dodecyl sulfate to 0.1% causes the second component's lifetime to increase by an average of a factor of 2.5. Only minor changes are observed in the first component's lifetime and the relative amplitudes of the two components. Two fractions isolated from the detergent-treated samples have also been examined. Our results indicate that excitation energy transfer within photosystem I is very efficient and that the excitation kinetics of the antennae may be limited by the trapping rate of P700 or strongly affected by the heterogeneity of the antennae.

AB - Picosecond time-resolved fluorescence measurements have been taken on a detergent-free P700-enriched complex at room temperature isolated from the blue-green alga Phormidium luridum with a chlorophyll a to reaction center ratio of 100. Emission at greater than 665 nm is characterized by two exponential-decay components. A fast component, which dominates the initial decay with an average lifetime of 16 ps and 87% amplitude, is attributed to excitations in the core antenna chlorophyll-proteins, which are rapidly trapped by the primary electron donor, P700. A second component, with an average lifetime of 106 ps and 13% amplitude, is attributed to the peripheral antenna proteins. For 532-nm, 30-ps pulse excitation the results are virtually independent of fluence in the range of 2 × 1012 to 4 × 1016 photons/cm2 and the oxidation state of P700. Addition of sodium dodecyl sulfate to 0.1% causes the second component's lifetime to increase by an average of a factor of 2.5. Only minor changes are observed in the first component's lifetime and the relative amplitudes of the two components. Two fractions isolated from the detergent-treated samples have also been examined. Our results indicate that excitation energy transfer within photosystem I is very efficient and that the excitation kinetics of the antennae may be limited by the trapping rate of P700 or strongly affected by the heterogeneity of the antennae.

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