Plant-based FRET biosensor discriminates environmental zinc levels

Joshua P. Adams, Ardeshir Adeli, Chuan Yu Hsu, Richard L. Harkess, Grier P. Page, Claude W. dePamphilis, Emily B. Schultz, Cetin Yuceer

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Heavy metal accumulation in the environment poses great risks to flora and fauna. However, monitoring sites prone to accumulation poses scale and economic challenges. In this study, we present and test a method for monitoring these sites using fluorescent resonance energy transfer (FRET) change in response to zinc (Zn) accumulation in plants as a proxy for environmental health. We modified a plant Zn transport protein by adding flanking fluorescent proteins (FPs) and deploying the construct into two different species. In Arabidopsis thaliana, FRET was monitored by a confocal microscope and had a 1.4-fold increase in intensity as the metal concentration increased. This led to a 16.7% overall error-rate when discriminating between a control (1μm Zn) and high (10mm Zn) treatment after 96h. The second host plant (Populus tremula×Populu salba) also had greater FRET values (1.3-fold increase) when exposed to the higher concentration of Zn, while overall error-rates were greater at 22.4%. These results indicate that as plants accumulate Zn, protein conformational changes occur in response to Zn causing differing interaction between FPs. This results in greater FRET values when exposed to greater amounts of Zn and monitored with appropriate light sources and filters. We also demonstrate how this construct can be moved into different host plants effectively including one tree species. This chimeric protein potentially offers a method for monitoring large areas of land for Zn accumulation, is transferable among species, and could be modified to monitor other specific heavy metals that pose environmental risks.

Original languageEnglish (US)
Pages (from-to)207-216
Number of pages10
JournalPlant Biotechnology Journal
Volume10
Issue number2
DOIs
StatePublished - Feb 1 2012

Fingerprint

biosensors
Energy Transfer
Biosensing Techniques
energy transfer
Zinc
zinc
monitoring
Heavy Metals
Proteins
heavy metals
host plants
recombinant fusion proteins
Populus
Environmental Health
transport proteins
Proxy
Arabidopsis
microscopes
Carrier Proteins
Arabidopsis thaliana

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Agronomy and Crop Science
  • Plant Science

Cite this

Adams, J. P., Adeli, A., Hsu, C. Y., Harkess, R. L., Page, G. P., dePamphilis, C. W., ... Yuceer, C. (2012). Plant-based FRET biosensor discriminates environmental zinc levels. Plant Biotechnology Journal, 10(2), 207-216. https://doi.org/10.1111/j.1467-7652.2011.00656.x
Adams, Joshua P. ; Adeli, Ardeshir ; Hsu, Chuan Yu ; Harkess, Richard L. ; Page, Grier P. ; dePamphilis, Claude W. ; Schultz, Emily B. ; Yuceer, Cetin. / Plant-based FRET biosensor discriminates environmental zinc levels. In: Plant Biotechnology Journal. 2012 ; Vol. 10, No. 2. pp. 207-216.
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Adams, JP, Adeli, A, Hsu, CY, Harkess, RL, Page, GP, dePamphilis, CW, Schultz, EB & Yuceer, C 2012, 'Plant-based FRET biosensor discriminates environmental zinc levels', Plant Biotechnology Journal, vol. 10, no. 2, pp. 207-216. https://doi.org/10.1111/j.1467-7652.2011.00656.x

Plant-based FRET biosensor discriminates environmental zinc levels. / Adams, Joshua P.; Adeli, Ardeshir; Hsu, Chuan Yu; Harkess, Richard L.; Page, Grier P.; dePamphilis, Claude W.; Schultz, Emily B.; Yuceer, Cetin.

In: Plant Biotechnology Journal, Vol. 10, No. 2, 01.02.2012, p. 207-216.

Research output: Contribution to journalArticle

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