@article{3d08db232b8c4a758d5513cb655e38f1,
title = "Plant homeodomain finger protein 2 as a novel IKAROS target in acute lymphoblastic leukemia",
abstract = "Aim: Clinical significance of plant homeodomain finger 2 (PHF2) expressions is explored in acute lymphoblastic leukemia (ALL) patients. Methods: mRNA level was examined by qPCR. The retroviral gene expression, shRNA knockdown and chromatin-immunoprecipitation are used to observe IKAROS regulation on PHF2 transcription. Results: PHF2 expression is significantly reduced in subsets of ALL patients, and PHF2 low expression correlates with leukemia cell proliferation and an elevation of several poor prognostic markers in B-cell ALL. IKAROS directly promotes PHF2 expression and patients with IKAROS deletion have significantly lower PHF2 expression. Casein kinase II (CK2) inhibitor significantly promotes PHF2 expression in an IKAROS-dependent manner, and casein kinase II inhibitor treatment also results in an increase of PHF2 expression and enrichment of IKAROS and H3K4me3 at PHF2 promoter in primary cells. Conclusion: Our results demonstrate that the IKAROS promotes PHF2 expression, and suggest that PHF2 low expression works with the IKAROS gene deletion to drive oncogenesis of ALL.",
author = "Zheng Ge and Yan Gu and Qi Han and Justin Sloane and Qinyu Ge and Goufeng Gao and Jinlong Ma and Huihui Song and Jiaojiao Hu and Baoan Chen and Sinisa Dovat and Chunhua Song",
note = "Funding Information: This work is supported in part by Milstein Medical Asian American Partnership (MMAAP) Foundation Research Project Award in Hematology (2017); The National Natural Science Foundation of China (81770172, 81270613); The Key Research & Technology Projects in Jiangsu Province (BE2017747); The Fundamental Research Funds for the Central Universities (2242017K40271, 2242016K40143); Jiangsu Province Key Medical Talents (RC2011077); The Scientific Research Foundation for the Returned Overseas Chinese Scholars; State Education Ministry (39th); China Postdoctoral Science Foundation (20090461134); Special grade of the financial support from China Postdoctoral Science Foundation (201003598); The Six Great Talent Peak Plan of Jiangsu (2010-WS-024) (to Z Ge). This work has also been partially supported by NIH, National Cancer Institute grants (R01CA209829, R01CA213912), Hyundai Hope on Wheels Scholar Grant, the Four Diamonds Fund of the Pennsylvania State University College of Medicine (to S Dovat and C Song); Bear Necessities Pediatric Cancer Foundation, Alex{\textquoteright}s Lemonade Stand Foundation and the John Wawrynovic Leukemia Research Scholar Endowment (to S Dovat). The authors have no other relevant affiliations or financial involvement with any organization or entity with a financial interest in or financial conflict with the subject matter or materials discussed in the manuscript apart from those disclosed. Publisher Copyright: {\textcopyright} 2017 Future Medicine Ltd.",
year = "2018",
month = jan,
doi = "10.2217/epi-2017-0092",
language = "English (US)",
volume = "10",
pages = "59--69",
journal = "Epigenomics",
issn = "1750-1911",
publisher = "Future Medicine Ltd.",
number = "1",
}