Plant homeodomain finger protein 2 as a novel IKAROS target in acute lymphoblastic leukemia

Zheng Ge, Yan Gu, Qi Han, Justin Sloane, Qinyu Ge, Goufeng Gao, Jinlong Ma, Huihui Song, Jiaojiao Hu, Baoan Chen, Sinisa Dovat, Chunhua Song

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Aim: Clinical significance of plant homeodomain finger 2 (PHF2) expressions is explored in acute lymphoblastic leukemia (ALL) patients. Methods: mRNA level was examined by qPCR. The retroviral gene expression, shRNA knockdown and chromatin-immunoprecipitation are used to observe IKAROS regulation on PHF2 transcription. Results: PHF2 expression is significantly reduced in subsets of ALL patients, and PHF2 low expression correlates with leukemia cell proliferation and an elevation of several poor prognostic markers in B-cell ALL. IKAROS directly promotes PHF2 expression and patients with IKAROS deletion have significantly lower PHF2 expression. Casein kinase II (CK2) inhibitor significantly promotes PHF2 expression in an IKAROS-dependent manner, and casein kinase II inhibitor treatment also results in an increase of PHF2 expression and enrichment of IKAROS and H3K4me3 at PHF2 promoter in primary cells. Conclusion: Our results demonstrate that the IKAROS promotes PHF2 expression, and suggest that PHF2 low expression works with the IKAROS gene deletion to drive oncogenesis of ALL.

Original languageEnglish (US)
Pages (from-to)59-69
Number of pages11
JournalEpigenomics
Volume10
Issue number1
DOIs
StatePublished - Jan 2018

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Homeodomain Proteins
Plant Proteins
Precursor Cell Lymphoblastic Leukemia-Lymphoma
Casein Kinase II
Chromatin Immunoprecipitation
Gene Deletion
Small Interfering RNA
Carcinogenesis
Leukemia
B-Lymphocytes
Cell Proliferation

All Science Journal Classification (ASJC) codes

  • Genetics
  • Cancer Research

Cite this

Ge, Z., Gu, Y., Han, Q., Sloane, J., Ge, Q., Gao, G., ... Song, C. (2018). Plant homeodomain finger protein 2 as a novel IKAROS target in acute lymphoblastic leukemia. Epigenomics, 10(1), 59-69. https://doi.org/10.2217/epi-2017-0092
Ge, Zheng ; Gu, Yan ; Han, Qi ; Sloane, Justin ; Ge, Qinyu ; Gao, Goufeng ; Ma, Jinlong ; Song, Huihui ; Hu, Jiaojiao ; Chen, Baoan ; Dovat, Sinisa ; Song, Chunhua. / Plant homeodomain finger protein 2 as a novel IKAROS target in acute lymphoblastic leukemia. In: Epigenomics. 2018 ; Vol. 10, No. 1. pp. 59-69.
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abstract = "Aim: Clinical significance of plant homeodomain finger 2 (PHF2) expressions is explored in acute lymphoblastic leukemia (ALL) patients. Methods: mRNA level was examined by qPCR. The retroviral gene expression, shRNA knockdown and chromatin-immunoprecipitation are used to observe IKAROS regulation on PHF2 transcription. Results: PHF2 expression is significantly reduced in subsets of ALL patients, and PHF2 low expression correlates with leukemia cell proliferation and an elevation of several poor prognostic markers in B-cell ALL. IKAROS directly promotes PHF2 expression and patients with IKAROS deletion have significantly lower PHF2 expression. Casein kinase II (CK2) inhibitor significantly promotes PHF2 expression in an IKAROS-dependent manner, and casein kinase II inhibitor treatment also results in an increase of PHF2 expression and enrichment of IKAROS and H3K4me3 at PHF2 promoter in primary cells. Conclusion: Our results demonstrate that the IKAROS promotes PHF2 expression, and suggest that PHF2 low expression works with the IKAROS gene deletion to drive oncogenesis of ALL.",
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Ge, Z, Gu, Y, Han, Q, Sloane, J, Ge, Q, Gao, G, Ma, J, Song, H, Hu, J, Chen, B, Dovat, S & Song, C 2018, 'Plant homeodomain finger protein 2 as a novel IKAROS target in acute lymphoblastic leukemia', Epigenomics, vol. 10, no. 1, pp. 59-69. https://doi.org/10.2217/epi-2017-0092

Plant homeodomain finger protein 2 as a novel IKAROS target in acute lymphoblastic leukemia. / Ge, Zheng; Gu, Yan; Han, Qi; Sloane, Justin; Ge, Qinyu; Gao, Goufeng; Ma, Jinlong; Song, Huihui; Hu, Jiaojiao; Chen, Baoan; Dovat, Sinisa; Song, Chunhua.

In: Epigenomics, Vol. 10, No. 1, 01.2018, p. 59-69.

Research output: Contribution to journalArticle

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AU - Ge, Zheng

AU - Gu, Yan

AU - Han, Qi

AU - Sloane, Justin

AU - Ge, Qinyu

AU - Gao, Goufeng

AU - Ma, Jinlong

AU - Song, Huihui

AU - Hu, Jiaojiao

AU - Chen, Baoan

AU - Dovat, Sinisa

AU - Song, Chunhua

PY - 2018/1

Y1 - 2018/1

N2 - Aim: Clinical significance of plant homeodomain finger 2 (PHF2) expressions is explored in acute lymphoblastic leukemia (ALL) patients. Methods: mRNA level was examined by qPCR. The retroviral gene expression, shRNA knockdown and chromatin-immunoprecipitation are used to observe IKAROS regulation on PHF2 transcription. Results: PHF2 expression is significantly reduced in subsets of ALL patients, and PHF2 low expression correlates with leukemia cell proliferation and an elevation of several poor prognostic markers in B-cell ALL. IKAROS directly promotes PHF2 expression and patients with IKAROS deletion have significantly lower PHF2 expression. Casein kinase II (CK2) inhibitor significantly promotes PHF2 expression in an IKAROS-dependent manner, and casein kinase II inhibitor treatment also results in an increase of PHF2 expression and enrichment of IKAROS and H3K4me3 at PHF2 promoter in primary cells. Conclusion: Our results demonstrate that the IKAROS promotes PHF2 expression, and suggest that PHF2 low expression works with the IKAROS gene deletion to drive oncogenesis of ALL.

AB - Aim: Clinical significance of plant homeodomain finger 2 (PHF2) expressions is explored in acute lymphoblastic leukemia (ALL) patients. Methods: mRNA level was examined by qPCR. The retroviral gene expression, shRNA knockdown and chromatin-immunoprecipitation are used to observe IKAROS regulation on PHF2 transcription. Results: PHF2 expression is significantly reduced in subsets of ALL patients, and PHF2 low expression correlates with leukemia cell proliferation and an elevation of several poor prognostic markers in B-cell ALL. IKAROS directly promotes PHF2 expression and patients with IKAROS deletion have significantly lower PHF2 expression. Casein kinase II (CK2) inhibitor significantly promotes PHF2 expression in an IKAROS-dependent manner, and casein kinase II inhibitor treatment also results in an increase of PHF2 expression and enrichment of IKAROS and H3K4me3 at PHF2 promoter in primary cells. Conclusion: Our results demonstrate that the IKAROS promotes PHF2 expression, and suggest that PHF2 low expression works with the IKAROS gene deletion to drive oncogenesis of ALL.

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