Plant mitochondrial recombination surveillance requires unusual RecA and MutS homologs

Vikas Shedge, Maria Arrieta-Montiel, Alan C. Christensen, Sally A. Mackenzie

Research output: Contribution to journalArticle

157 Citations (Scopus)

Abstract

For >20 years, the enigmatic behavior of plant mitochondrial genomes has been well described but not well understood. Chimeric genes appear, and occasionally are differentially replicated or expressed, with significant effects on plant phenotype, most notably on male fertility, yet the mechanisms of DNA replication, chimera formation, and recombination have remained elusive. Using mutations in two important genes of mitochondrial DNA metabolism, we have observed reproducible asymmetric recombination events occurring at specific locations in the mitochondrial genome. Based on these experiments and existing models of double-strand break repair, we propose a model for plant mitochondrial DNA replication, chimeric gene formation, and the illegitimate recombination events that lead to stoichiometric changes. We also address the physiological and developmental effects of aberrant events in mitochondrial genome maintenance, showing that mitochondrial genome rearrangements, when controlled, influence plant reproduction, but when uncontrolled, lead to aberrant growth phenotypes and dramatic reduction of the cell cycle.

Original languageEnglish (US)
Pages (from-to)1251-1264
Number of pages14
JournalPlant Cell
Volume19
Issue number4
DOIs
StatePublished - Apr 1 2007

Fingerprint

Mitochondrial Genome
Genetic Recombination
monitoring
DNA replication
DNA Replication
Mitochondrial DNA
mitochondrial DNA
Plant DNA
Genes
Plant Genome
Phenotype
phenotype
plant reproduction
genes
chimerism
male fertility
Reproduction
Fertility
cell cycle
Cell Cycle

All Science Journal Classification (ASJC) codes

  • Plant Science
  • Cell Biology

Cite this

Shedge, Vikas ; Arrieta-Montiel, Maria ; Christensen, Alan C. ; Mackenzie, Sally A. / Plant mitochondrial recombination surveillance requires unusual RecA and MutS homologs. In: Plant Cell. 2007 ; Vol. 19, No. 4. pp. 1251-1264.
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Plant mitochondrial recombination surveillance requires unusual RecA and MutS homologs. / Shedge, Vikas; Arrieta-Montiel, Maria; Christensen, Alan C.; Mackenzie, Sally A.

In: Plant Cell, Vol. 19, No. 4, 01.04.2007, p. 1251-1264.

Research output: Contribution to journalArticle

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