We compared two different culture media for high frequency callus induction and plant regeneration from cultured immature embryos (excised scutella) of 14 sorghum genotypes. Embryogenic calli were induced in 10-15 days after the embryos were plated on LS medium with 0.25 mg l-1 zeatin and 0.5 mg l-1 2,4-D. For induction of embryogenic callus, this media was better than the other with 2,4-D alone. Regeneration was achieved by transferring embryonic calli to MS medium containing 2 mg l-1 NAA, 0.5 mg l-1 BAP and 0.1 mg l-1 GA3. Both callusing and regenerating ability varied (10-100%) among the genotypes. Popular seed parents 296 B and BTx 623 showed 100% callus induction and regeneration. The anatomical studies on embryogenic calli derived from scutellum revealed 4-6 pro-embryos from each scutellum, with secondary embryos initiating around the primary ones after a month under culture. The above protocols are appropriate for use of immature embryos to genetically transform wide range of sorghum genotypes.
All Science Journal Classification (ASJC) codes
- Agronomy and Crop Science