Plasma biomarkers associated with ALS and their relationship to iron homeostasis

Ryan M. Mitchell, Zachary Simmons, John L. Beard, Helen E. Stephens, James R. Connor

Research output: Contribution to journalArticle

60 Citations (Scopus)

Abstract

Amyotrophic lateral sclerosis (ALS) is a progressive neurodegenerative disease with complicated pathogenesis with variable presentation and disease progression. There is a critical need for a panel of biomarkers to provide clinicians and researchers with additional information. In this study, multiplex immunoassays were used to screen a number of cytokines, growth factors, and iron-related proteins. ALS patients had significantly higher plasma levels of L-ferritin and lower concentrations of transferrin when compared to healthy controls and together classified a test group of subjects with 82% accuracy. Duration of ALS symptoms correlated positively with levels of monocyte chemoattractant protein 1 (MCP-1) and negatively with levels of granulocyte-macrophage colony stimulating factor (GM-CSF). The biomarker profile suggests iron homeostasis is disrupted in ALS patients, and changes in ferritin and transferrin (Tf) appear to be indicators of ongoing inflammatory processes. The data demonstrate a plasma biomarker profile in ALS patients that may differ from published reports of cerebrospinal fluid biomarkers.

Original languageEnglish (US)
Pages (from-to)95-103
Number of pages9
JournalMuscle and Nerve
Volume42
Issue number1
DOIs
StatePublished - Jul 1 2010

Fingerprint

Amyotrophic Lateral Sclerosis
Homeostasis
Iron
Biomarkers
Transferrin
Apoferritins
Chemokine CCL2
Granulocyte-Macrophage Colony-Stimulating Factor
Ferritins
Immunoassay
Neurodegenerative Diseases
Cerebrospinal Fluid
Disease Progression
Intercellular Signaling Peptides and Proteins
Research Personnel
Cytokines
Proteins

All Science Journal Classification (ASJC) codes

  • Physiology
  • Clinical Neurology
  • Cellular and Molecular Neuroscience
  • Physiology (medical)

Cite this

Mitchell, Ryan M. ; Simmons, Zachary ; Beard, John L. ; Stephens, Helen E. ; Connor, James R. / Plasma biomarkers associated with ALS and their relationship to iron homeostasis. In: Muscle and Nerve. 2010 ; Vol. 42, No. 1. pp. 95-103.
@article{f369ef25861b48c7ae64b7a6a20a3df9,
title = "Plasma biomarkers associated with ALS and their relationship to iron homeostasis",
abstract = "Amyotrophic lateral sclerosis (ALS) is a progressive neurodegenerative disease with complicated pathogenesis with variable presentation and disease progression. There is a critical need for a panel of biomarkers to provide clinicians and researchers with additional information. In this study, multiplex immunoassays were used to screen a number of cytokines, growth factors, and iron-related proteins. ALS patients had significantly higher plasma levels of L-ferritin and lower concentrations of transferrin when compared to healthy controls and together classified a test group of subjects with 82{\%} accuracy. Duration of ALS symptoms correlated positively with levels of monocyte chemoattractant protein 1 (MCP-1) and negatively with levels of granulocyte-macrophage colony stimulating factor (GM-CSF). The biomarker profile suggests iron homeostasis is disrupted in ALS patients, and changes in ferritin and transferrin (Tf) appear to be indicators of ongoing inflammatory processes. The data demonstrate a plasma biomarker profile in ALS patients that may differ from published reports of cerebrospinal fluid biomarkers.",
author = "Mitchell, {Ryan M.} and Zachary Simmons and Beard, {John L.} and Stephens, {Helen E.} and Connor, {James R.}",
year = "2010",
month = "7",
day = "1",
doi = "10.1002/mus.21625",
language = "English (US)",
volume = "42",
pages = "95--103",
journal = "Muscle and Nerve",
issn = "0148-639X",
publisher = "John Wiley and Sons Inc.",
number = "1",

}

Plasma biomarkers associated with ALS and their relationship to iron homeostasis. / Mitchell, Ryan M.; Simmons, Zachary; Beard, John L.; Stephens, Helen E.; Connor, James R.

In: Muscle and Nerve, Vol. 42, No. 1, 01.07.2010, p. 95-103.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Plasma biomarkers associated with ALS and their relationship to iron homeostasis

AU - Mitchell, Ryan M.

AU - Simmons, Zachary

AU - Beard, John L.

AU - Stephens, Helen E.

AU - Connor, James R.

PY - 2010/7/1

Y1 - 2010/7/1

N2 - Amyotrophic lateral sclerosis (ALS) is a progressive neurodegenerative disease with complicated pathogenesis with variable presentation and disease progression. There is a critical need for a panel of biomarkers to provide clinicians and researchers with additional information. In this study, multiplex immunoassays were used to screen a number of cytokines, growth factors, and iron-related proteins. ALS patients had significantly higher plasma levels of L-ferritin and lower concentrations of transferrin when compared to healthy controls and together classified a test group of subjects with 82% accuracy. Duration of ALS symptoms correlated positively with levels of monocyte chemoattractant protein 1 (MCP-1) and negatively with levels of granulocyte-macrophage colony stimulating factor (GM-CSF). The biomarker profile suggests iron homeostasis is disrupted in ALS patients, and changes in ferritin and transferrin (Tf) appear to be indicators of ongoing inflammatory processes. The data demonstrate a plasma biomarker profile in ALS patients that may differ from published reports of cerebrospinal fluid biomarkers.

AB - Amyotrophic lateral sclerosis (ALS) is a progressive neurodegenerative disease with complicated pathogenesis with variable presentation and disease progression. There is a critical need for a panel of biomarkers to provide clinicians and researchers with additional information. In this study, multiplex immunoassays were used to screen a number of cytokines, growth factors, and iron-related proteins. ALS patients had significantly higher plasma levels of L-ferritin and lower concentrations of transferrin when compared to healthy controls and together classified a test group of subjects with 82% accuracy. Duration of ALS symptoms correlated positively with levels of monocyte chemoattractant protein 1 (MCP-1) and negatively with levels of granulocyte-macrophage colony stimulating factor (GM-CSF). The biomarker profile suggests iron homeostasis is disrupted in ALS patients, and changes in ferritin and transferrin (Tf) appear to be indicators of ongoing inflammatory processes. The data demonstrate a plasma biomarker profile in ALS patients that may differ from published reports of cerebrospinal fluid biomarkers.

UR - http://www.scopus.com/inward/record.url?scp=77954129033&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=77954129033&partnerID=8YFLogxK

U2 - 10.1002/mus.21625

DO - 10.1002/mus.21625

M3 - Article

C2 - 20544912

AN - SCOPUS:77954129033

VL - 42

SP - 95

EP - 103

JO - Muscle and Nerve

JF - Muscle and Nerve

SN - 0148-639X

IS - 1

ER -