Plasma cholesterol esterifying activity in guinea pigs

R. Ostwald, M. Crim, Michael Henry Green, M. Meng

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Plasma cholesterol esterifying activity has been measured in guinea pigs fed either a control diet or the same diet supplemented with 1% cholesterol. The extent of esterification was found to be similar in the cholesterol-fed and control guinea pigs and somewhat lower than in rats. The initial rate of esterification was also of the same magnitude as that found in rats and humans, and unaffected by dietary cholesterol if autologous plasma was used as substrate. However, LCAT activity from cholesterol-fed guinea pigs was significantly higher than that of control plasma when acting on either control or cholesterol-fed substrate. This suggests that dietary cholesterol increases the amount (or activity) of LCAT but that the substrate is unsuitable or that a necessary cofactor is present in limiting amounts. Heat treatment of guinea pig plasma seems to alter substrate availability to varying degrees. The implications of these findings in relation to substrate specificity and cofactor requirements of guinea pig LCAT are discussed.

Original languageEnglish (US)
Pages (from-to)42-50
Number of pages9
JournalNutrition and Metabolism
Volume23
Issue number1
StatePublished - Dec 1 1978

Fingerprint

guinea pigs
Guinea Pigs
Thermodynamic properties
Cholesterol
cholesterol
Plasmas
Substrates
Dietary Cholesterol
Esterification
Nutrition
Rats
esterification
Diet
Substrate Specificity
rats
Hot Temperature
Heat treatment
substrate specificity
Availability
diet

All Science Journal Classification (ASJC) codes

  • Food Science
  • Medicine (miscellaneous)
  • Biochemistry
  • Endocrinology

Cite this

Ostwald, R., Crim, M., Green, M. H., & Meng, M. (1978). Plasma cholesterol esterifying activity in guinea pigs. Nutrition and Metabolism, 23(1), 42-50.
Ostwald, R. ; Crim, M. ; Green, Michael Henry ; Meng, M. / Plasma cholesterol esterifying activity in guinea pigs. In: Nutrition and Metabolism. 1978 ; Vol. 23, No. 1. pp. 42-50.
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Ostwald, R, Crim, M, Green, MH & Meng, M 1978, 'Plasma cholesterol esterifying activity in guinea pigs', Nutrition and Metabolism, vol. 23, no. 1, pp. 42-50.

Plasma cholesterol esterifying activity in guinea pigs. / Ostwald, R.; Crim, M.; Green, Michael Henry; Meng, M.

In: Nutrition and Metabolism, Vol. 23, No. 1, 01.12.1978, p. 42-50.

Research output: Contribution to journalArticle

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AB - Plasma cholesterol esterifying activity has been measured in guinea pigs fed either a control diet or the same diet supplemented with 1% cholesterol. The extent of esterification was found to be similar in the cholesterol-fed and control guinea pigs and somewhat lower than in rats. The initial rate of esterification was also of the same magnitude as that found in rats and humans, and unaffected by dietary cholesterol if autologous plasma was used as substrate. However, LCAT activity from cholesterol-fed guinea pigs was significantly higher than that of control plasma when acting on either control or cholesterol-fed substrate. This suggests that dietary cholesterol increases the amount (or activity) of LCAT but that the substrate is unsuitable or that a necessary cofactor is present in limiting amounts. Heat treatment of guinea pig plasma seems to alter substrate availability to varying degrees. The implications of these findings in relation to substrate specificity and cofactor requirements of guinea pig LCAT are discussed.

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Ostwald R, Crim M, Green MH, Meng M. Plasma cholesterol esterifying activity in guinea pigs. Nutrition and Metabolism. 1978 Dec 1;23(1):42-50.