Plasma-treated, collagen-anchored polylactone: Its cell affinity evaluation under shear or shear-free conditions

Jian Yang, Yuqing Wan, Junlin Yang, Jianzhong Bei, Shenguo Wang

Research output: Contribution to journalArticle

62 Citations (Scopus)

Abstract

Poly(L-lactic acid)(PLLA) and poly(L-lactic-co-glycolic acid) (PLGA) (85/15) were modified by plasma treatment. Then they were collagen anchored (PT/CA), and the cell affinity was evaluated by cell culture under shear or shear-free conditions. A convenient and "intuitionistic" dyeing method has been proposed for measuring the modified depth when plasma treatment is applied for the treatment of porous scaffolds. A parallel plate flow chamber was developed in order to study the cell affinity of a material under shear stress. Our results show that a porous scaffold can be modified by plasma treatment and that a depth of about 4.0 mm for this modification can be reached with NH3 plasma treatment (50 w, 20 Pa, 5 min). PT/CA modification is an effective surface modification method for facilitating cell transplantation and improving the cell affinity of three-dimensional porous cell scaffolds in tissue engineering. It can solve the problem of non-uniform cell distribution in most synthetic porous cell scaffolds. Using the flow chamber system, a series of quantitative data, including cell adherent fraction, cell area, and mean shape, were compared to evaluate the cell affinity of PLLA before and after PT/CA modification. The results indicate that the quality of cell attachment on PT/CA-modified PLLA apparently is better than that on unmodified PLLA. The flow chamber system potentially may be a tool for evaluating surface modification methods.

Original languageEnglish (US)
Pages (from-to)1139-1147
Number of pages9
JournalJournal of Biomedical Materials Research - Part A
Volume67
Issue number4
DOIs
StatePublished - Dec 15 2003

Fingerprint

Lactic acid
Collagen
Scaffolds
Plasmas
glycolic acid
Surface treatment
Scaffolds (biology)
Tissue engineering
Cell culture
Dyeing
Shear stress
Acids
poly(lactic acid)

All Science Journal Classification (ASJC) codes

  • Ceramics and Composites
  • Biomaterials
  • Biomedical Engineering
  • Metals and Alloys

Cite this

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abstract = "Poly(L-lactic acid)(PLLA) and poly(L-lactic-co-glycolic acid) (PLGA) (85/15) were modified by plasma treatment. Then they were collagen anchored (PT/CA), and the cell affinity was evaluated by cell culture under shear or shear-free conditions. A convenient and {"}intuitionistic{"} dyeing method has been proposed for measuring the modified depth when plasma treatment is applied for the treatment of porous scaffolds. A parallel plate flow chamber was developed in order to study the cell affinity of a material under shear stress. Our results show that a porous scaffold can be modified by plasma treatment and that a depth of about 4.0 mm for this modification can be reached with NH3 plasma treatment (50 w, 20 Pa, 5 min). PT/CA modification is an effective surface modification method for facilitating cell transplantation and improving the cell affinity of three-dimensional porous cell scaffolds in tissue engineering. It can solve the problem of non-uniform cell distribution in most synthetic porous cell scaffolds. Using the flow chamber system, a series of quantitative data, including cell adherent fraction, cell area, and mean shape, were compared to evaluate the cell affinity of PLLA before and after PT/CA modification. The results indicate that the quality of cell attachment on PT/CA-modified PLLA apparently is better than that on unmodified PLLA. The flow chamber system potentially may be a tool for evaluating surface modification methods.",
author = "Jian Yang and Yuqing Wan and Junlin Yang and Jianzhong Bei and Shenguo Wang",
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Plasma-treated, collagen-anchored polylactone : Its cell affinity evaluation under shear or shear-free conditions. / Yang, Jian; Wan, Yuqing; Yang, Junlin; Bei, Jianzhong; Wang, Shenguo.

In: Journal of Biomedical Materials Research - Part A, Vol. 67, No. 4, 15.12.2003, p. 1139-1147.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Plasma-treated, collagen-anchored polylactone

T2 - Its cell affinity evaluation under shear or shear-free conditions

AU - Yang, Jian

AU - Wan, Yuqing

AU - Yang, Junlin

AU - Bei, Jianzhong

AU - Wang, Shenguo

PY - 2003/12/15

Y1 - 2003/12/15

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AB - Poly(L-lactic acid)(PLLA) and poly(L-lactic-co-glycolic acid) (PLGA) (85/15) were modified by plasma treatment. Then they were collagen anchored (PT/CA), and the cell affinity was evaluated by cell culture under shear or shear-free conditions. A convenient and "intuitionistic" dyeing method has been proposed for measuring the modified depth when plasma treatment is applied for the treatment of porous scaffolds. A parallel plate flow chamber was developed in order to study the cell affinity of a material under shear stress. Our results show that a porous scaffold can be modified by plasma treatment and that a depth of about 4.0 mm for this modification can be reached with NH3 plasma treatment (50 w, 20 Pa, 5 min). PT/CA modification is an effective surface modification method for facilitating cell transplantation and improving the cell affinity of three-dimensional porous cell scaffolds in tissue engineering. It can solve the problem of non-uniform cell distribution in most synthetic porous cell scaffolds. Using the flow chamber system, a series of quantitative data, including cell adherent fraction, cell area, and mean shape, were compared to evaluate the cell affinity of PLLA before and after PT/CA modification. The results indicate that the quality of cell attachment on PT/CA-modified PLLA apparently is better than that on unmodified PLLA. The flow chamber system potentially may be a tool for evaluating surface modification methods.

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