Plasmalemmal and vesicular γ-aminobutyric acid transporter expression in the developing mouse retina

Chenying Guo, Salvatore L. Stella, Arlene A. Hirano, Nicholas C. Brecha

Research output: Contribution to journalArticle

36 Citations (Scopus)

Abstract

Plasmalemmal and vesicular γ-aminobutyric acid (GABA) transporters influence neurotransmission by regulating high-affinity GABA uptake and GABA release into the synaptic cleft and extracellular space. Postnatal expression of the plasmalemmal GABA transporter-1 (GAT-1), GAT-3, and the vesicular GABA/glycine transporter (VGAT) were evaluated in the developing mouse retina by using immunohistochemistry with affinity-purified antibodies. Weak transporter immunoreactivity was observed in the inner retina at postnatal day 0 (P0). GAT-1 immunostaining at P0 and at older ages was in amacrine and displaced amacrine cells in the inner nuclear layer (INL) and ganglion cell layer (GCL), respectively, and in their processes in the inner plexiform layer (IPL). At P10, weak GAT-1 immunostaining was in Müller cell processes. GAT-3 immunostaining at P0 and older ages was in amacrine cells and their processes, as well as in Müller cells and their processes that extended radially across the retina. At P10, Müller cell somata were observed in the middle of the INL. VGAT immunostaining was present at P0 and older ages in amacrine cells in the INL as well as processes in the IPL. At P5, weak VGAT immunostaining was also observed in horizontal cell somata and processes. By P15, the GAT and VGAT immunostaining patterns appear similar to the adult immunostaining patterns; they reached adult levels by about P20. These findings demonstrate that GABA uptake and release are initially established in the inner retina during the first postnatal week and that these systems subsequently mature in the outer retina during the second postnatal week.

Original languageEnglish (US)
Pages (from-to)6-26
Number of pages21
JournalJournal of Comparative Neurology
Volume512
Issue number1
DOIs
StatePublished - Jan 1 2009

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Aminobutyrates
Glycine Plasma Membrane Transport Proteins
GABA Plasma Membrane Transport Proteins
Retina
Amacrine Cells
gamma-Aminobutyric Acid
Carisoprodol
Antibody Affinity
Extracellular Space
Synaptic Transmission
Ganglia
Immunohistochemistry
vesicular GABA transporter
GAT

All Science Journal Classification (ASJC) codes

  • Neuroscience(all)

Cite this

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abstract = "Plasmalemmal and vesicular γ-aminobutyric acid (GABA) transporters influence neurotransmission by regulating high-affinity GABA uptake and GABA release into the synaptic cleft and extracellular space. Postnatal expression of the plasmalemmal GABA transporter-1 (GAT-1), GAT-3, and the vesicular GABA/glycine transporter (VGAT) were evaluated in the developing mouse retina by using immunohistochemistry with affinity-purified antibodies. Weak transporter immunoreactivity was observed in the inner retina at postnatal day 0 (P0). GAT-1 immunostaining at P0 and at older ages was in amacrine and displaced amacrine cells in the inner nuclear layer (INL) and ganglion cell layer (GCL), respectively, and in their processes in the inner plexiform layer (IPL). At P10, weak GAT-1 immunostaining was in M{\"u}ller cell processes. GAT-3 immunostaining at P0 and older ages was in amacrine cells and their processes, as well as in M{\"u}ller cells and their processes that extended radially across the retina. At P10, M{\"u}ller cell somata were observed in the middle of the INL. VGAT immunostaining was present at P0 and older ages in amacrine cells in the INL as well as processes in the IPL. At P5, weak VGAT immunostaining was also observed in horizontal cell somata and processes. By P15, the GAT and VGAT immunostaining patterns appear similar to the adult immunostaining patterns; they reached adult levels by about P20. These findings demonstrate that GABA uptake and release are initially established in the inner retina during the first postnatal week and that these systems subsequently mature in the outer retina during the second postnatal week.",
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Plasmalemmal and vesicular γ-aminobutyric acid transporter expression in the developing mouse retina. / Guo, Chenying; Stella, Salvatore L.; Hirano, Arlene A.; Brecha, Nicholas C.

In: Journal of Comparative Neurology, Vol. 512, No. 1, 01.01.2009, p. 6-26.

Research output: Contribution to journalArticle

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AU - Guo, Chenying

AU - Stella, Salvatore L.

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