Plasmid-coded DNA fragment developed as a specific gene probe for the identification of enteroaggregative Escherichia coli

C. Debroy, B. D. Bright, R. A. Wilson, J. Yealy, R. Kumar, M. K. Bhan

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Abstract

Enteroaggregative Escherichia coli (EAggEC) is a recently discovered diarrhoeal pathogen implicated as a cause of persistent diarrhoea in children. EAggEC strains exhibit a characteristic pattern of adherence when incubated with HEp-2 cells. Because of the difficulty in identifying this group of bacteria, the epidemiological significance of this pathogen as a diarrhoeal agent has not been fully realised. A gene probe was developed from the 60-MDa plasmid associated with EAggEC strains that encodes the genes for adherence and fimbriae. The sensitivity of the gene probe was 93% and the specificity 98% for detecting EAggEC isolates and is potentially useful for diagnostic and epidemiological studies.

Original languageEnglish (US)
Pages (from-to)393-398
Number of pages6
JournalJournal of Medical Microbiology
Volume41
Issue number6
DOIs
Publication statusPublished - Jan 1 1994

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All Science Journal Classification (ASJC) codes

  • Microbiology
  • Microbiology (medical)

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