Plasmin activity and complement activation during storage of citrated platelet concentrates

Arthur P. Bode, David T. Miller, Simon L. Newman, William J. Castellani, H. Thomas Norris

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27 Scopus citations


Platelet concentrates were studied for evidence of plasmin activity and complement activation during a 7-to-10-day storage period. When measured by an amidolytlc activity assay, plasmin reached a level of 845 ± 540 nkats/L on day 7 (n = 9). Fibrin(ogen) degradation product (FDP) levels became markedly elevated on the tenth day of storage, rising to 45 ± 22 μg/ml (n = 5). Antiplasmin levels decreased in platelet concentrates by 18% ± 6% (n = 5) over 7 days, but there was no significant decrease in stored platelet-poor plasma (-1.7%, n = 5, p = 0.5). The amount of plasminogen in platelet concentrate converted to plasmin was estimated to be less than 3% by assay of total plasminogen. Supernatant plasma from stored platelet concentrates was examined for the presence of the complement activation peptides C3a and C5a. From day 0 to day 10 of storage, mean C3a levels rose from 327 ng/ml to 6690 ng/ml. An equivalent increase in C3a levels, from 336 ng/ml at day 0 to 6866 ng/ml at day 10, was also observed in stored platelet-poor plasma. C5a was not detected (< 10 ng/ml) at any point during the storage period; however, we noted a small decrease of borderline significance (p = 0.04) in total C5 from day 0 (117 μg/ml) to day 10 (108 μg/ml). Only trace amounts of C3 fragments were found on stored platelets, and there was no evidence of the membrane attack complex. These findings indicate the presence of plasmin activity and conversion of C3 during storage of platelet concentrates.

Original languageEnglish (US)
Pages (from-to)94-102
Number of pages9
JournalThe Journal of Laboratory and Clinical Medicine
Issue number1
StatePublished - Jan 1989

All Science Journal Classification (ASJC) codes

  • Pathology and Forensic Medicine


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