Plasmodium falciparum: Development of a transgenic line for screening antimalarials using firefly luciferase as the reporter

Long Cui, Jun Miao, Jiaxu Wang, Qianjun Li, Liwang Cui

Research output: Contribution to journalArticle

28 Scopus citations

Abstract

High-throughput screening (HTS) of small-molecule libraries against pharmacological targets is a key strategy of contemporary drug discovery. This study reports a simple, robust, and cell-based luminescent method for assaying antimalarial drugs. Using transfection technology, we generated a stable Plasmodium falciparum line with high levels of firefly luciferase expression. A luciferase assay based on this parasite line was optimized in a 96-well plate format and used to compare with the standard [3H] hypoxanthine radioisotope method. The 50% inhibitory concentrations (IC50s) of chloroquine, artesunate, artemether, dihydroartemisinin and curcumin obtained by these two methods were not significantly different (P > 0.05, ANOVA). In addition, this assay could be performed conveniently with a luminescence plate reader using unsynchronized stages within as early as 12 h. Furthermore, the luciferase assay is robust with a Z′ score of 0.77-0.92, which suggests the feasibility for further miniaturization and automation.

Original languageEnglish (US)
Pages (from-to)80-87
Number of pages8
JournalExperimental Parasitology
Volume120
Issue number1
DOIs
StatePublished - Sep 1 2008

All Science Journal Classification (ASJC) codes

  • Parasitology
  • Immunology
  • Infectious Diseases

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