Plasmodium falciparum: Development of a transgenic line for screening antimalarials using firefly luciferase as the reporter

Long Cui, Jun Miao, Jiaxu Wang, Qianjun Li, Liwang Cui

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

High-throughput screening (HTS) of small-molecule libraries against pharmacological targets is a key strategy of contemporary drug discovery. This study reports a simple, robust, and cell-based luminescent method for assaying antimalarial drugs. Using transfection technology, we generated a stable Plasmodium falciparum line with high levels of firefly luciferase expression. A luciferase assay based on this parasite line was optimized in a 96-well plate format and used to compare with the standard [ 3H] hypoxanthine radioisotope method. The 50% inhibitory concentrations (IC 50s) of chloroquine, artesunate, artemether, dihydroartemisinin and curcumin obtained by these two methods were not significantly different (P > 0.05, ANOVA). In addition, this assay could be performed conveniently with a luminescence plate reader using unsynchronized stages within as early as 12 h. Furthermore, the luciferase assay is robust with a Z′ score of 0.77-0.92, which suggests the feasibility for further miniaturization and automation.

Original languageEnglish (US)
Pages (from-to)80-87
Number of pages8
JournalExperimental Parasitology
Volume120
Issue number1
DOIs
StatePublished - Sep 1 2008

Fingerprint

Firefly Luciferases
Antimalarials
Plasmodium falciparum
dihydroartemisinin
Luciferases
Small Molecule Libraries
Miniaturization
Hypoxanthine
Curcumin
Automation
Chloroquine
Drug Discovery
Luminescence
Radioisotopes
Inhibitory Concentration 50
Transfection
Analysis of Variance
Parasites
Pharmacology
Technology

All Science Journal Classification (ASJC) codes

  • Immunology
  • Parasitology
  • Infectious Diseases

Cite this

Cui, Long ; Miao, Jun ; Wang, Jiaxu ; Li, Qianjun ; Cui, Liwang. / Plasmodium falciparum : Development of a transgenic line for screening antimalarials using firefly luciferase as the reporter. In: Experimental Parasitology. 2008 ; Vol. 120, No. 1. pp. 80-87.
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Plasmodium falciparum : Development of a transgenic line for screening antimalarials using firefly luciferase as the reporter. / Cui, Long; Miao, Jun; Wang, Jiaxu; Li, Qianjun; Cui, Liwang.

In: Experimental Parasitology, Vol. 120, No. 1, 01.09.2008, p. 80-87.

Research output: Contribution to journalArticle

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