Plasmodium falciparum field isolates use complement receptor 1 (CR1) as a receptor for invasion of erythrocytes

Gordon A. Awandare, Carmenza Spadafora, J. Kathleen Moch, Sheetij Dutta, J. David Haynes, José A. Stoute

Research output: Contribution to journalArticle

22 Scopus citations

Abstract

A majority of Plasmodium falciparum strains invade erythrocytes through interactions with sialic acid (SA) on glycophorins. However, we recently reported that complement receptor 1 (CR1) is a SA-independent invasion receptor of many laboratory strains of P. falciparum. To determine the role of CR1 in erythrocyte invasion among P. falciparum field isolates, we tested eight isolates obtained from children in Kenya. All the parasites examined were capable of invading in a SA-independent manner, and invasion of neuraminidase-treated erythrocytes was nearly completely blocked by anti-CR1 and soluble CR1 (sCR1). In addition, anti-CR1 and sCR1 partially inhibited invasion of intact erythrocytes in a majority of isolates tested. Sequencing of the hypervariable region of P. falciparum AMA-1 showed considerable diversity among all the isolates. These data demonstrate that CR1 mediates SA-independent erythrocyte invasion in P. falciparum field isolates.

Original languageEnglish (US)
Pages (from-to)57-60
Number of pages4
JournalMolecular and biochemical parasitology
Volume177
Issue number1
DOIs
StatePublished - May 2011

All Science Journal Classification (ASJC) codes

  • Parasitology
  • Molecular Biology

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