Plasmodium falciparum histones induce endothelial proinflammatory response and barrier dysfunction

Mark R. Gillrie, Kristine Lee, Channe Gowda, Shevaun P. Davis, Marc Monestier, Liwang Cui, Tran Tinh Hien, Nicholas P.J. Day, May Ho

Research output: Contribution to journalArticle

49 Citations (Scopus)

Abstract

Plasmodium falciparum is a protozoan parasite of human erythrocytes that causes the most severe form of malaria. Severe P. falciparum infection is associated with endothelial activation and permeability, which are important determinants of the outcome of the infection. How endothelial cells become activated is not fully understood, particularly with regard to the effects of parasite subcomponents. We demonstrated that P. falciparum histones extracted from merozoites (HeH) directly stimulated the production of IL-8 and other inflammatory mediators by primary human dermal microvascular endothelial cells through a signaling pathway that involves Src family kinases and p38 MAPK. The stimulatory effect of HeH and recombinant P. falciparum H3 (PfH3) was abrogated by histone-specific antibodies. The release of nuclear contents on rupture of infected erythrocytes was captured by live cell imaging and confirmed by detecting nucleosomes in the supernatants of parasite cultures. HeH and recombinant parasite histones also induced endothelial permeability through a charge-dependent mechanism that resulted in disruption of junctional protein expression and cell death. Recombinant human activated protein C cleaved HeH and PfH3 and abrogated their proinflammatory effects. Circulating nucleosomes of both human and parasite origin were detected in the plasma of patients with falciparum malaria and correlated positively with disease severity. These results support a pathogenic role for both host- and pathogen-derived histones in P. falciparum-caused malaria.

Original languageEnglish (US)
Pages (from-to)1028-1039
Number of pages12
JournalAmerican Journal of Pathology
Volume180
Issue number3
DOIs
StatePublished - Jan 1 2012

Fingerprint

Plasmodium falciparum
Histones
Parasites
Nucleosomes
Falciparum Malaria
Malaria
Permeability
Endothelial Cells
Erythrocytes
Merozoites
src-Family Kinases
p38 Mitogen-Activated Protein Kinases
Protein C
Interleukin-8
Rupture
Cell Death
Skin
Antibodies
Infection
Proteins

All Science Journal Classification (ASJC) codes

  • Pathology and Forensic Medicine

Cite this

Gillrie, Mark R. ; Lee, Kristine ; Gowda, Channe ; Davis, Shevaun P. ; Monestier, Marc ; Cui, Liwang ; Hien, Tran Tinh ; Day, Nicholas P.J. ; Ho, May. / Plasmodium falciparum histones induce endothelial proinflammatory response and barrier dysfunction. In: American Journal of Pathology. 2012 ; Vol. 180, No. 3. pp. 1028-1039.
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Gillrie, MR, Lee, K, Gowda, C, Davis, SP, Monestier, M, Cui, L, Hien, TT, Day, NPJ & Ho, M 2012, 'Plasmodium falciparum histones induce endothelial proinflammatory response and barrier dysfunction', American Journal of Pathology, vol. 180, no. 3, pp. 1028-1039. https://doi.org/10.1016/j.ajpath.2011.11.037

Plasmodium falciparum histones induce endothelial proinflammatory response and barrier dysfunction. / Gillrie, Mark R.; Lee, Kristine; Gowda, Channe; Davis, Shevaun P.; Monestier, Marc; Cui, Liwang; Hien, Tran Tinh; Day, Nicholas P.J.; Ho, May.

In: American Journal of Pathology, Vol. 180, No. 3, 01.01.2012, p. 1028-1039.

Research output: Contribution to journalArticle

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