Plasmodium falciparum responds to amino acid starvation by entering into a hibernatory state

Shalon E. Babbitt, Lindsey Altenhofen, Simon A. Cobbold, Eva S. Istvan, Clare Fennellf, Christian Doerig, Manuel Llinás, Daniel E. Goldberg

Research output: Contribution to journalArticle

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Abstract

The human malaria parasite Plasmodium falciparum is auxotrophic for most amino acids. Its amino acid needs are met largely through the degradation of host erythrocyte hemoglobin; however the parasite must acquire isoleucine exogenously, because this amino acid is not present in adult human hemoglobin. We report that when isoleucine is withdrawn from the culture medium of intraerythrocytic P. falciparum, the parasite slows its metabolism and progresses through its developmental cycle at a reduced rate. Isoleucine- starved parasites remain viable for 72 h and resume rapid growth upon resupplementation. Protein degradation during starvation is important for maintenance of this hibernatory state. Microarray analysis of starved parasites revealed a 60% decrease in the rate of progression through the normal transcriptional program but no other apparent stress response. Plasmodium parasites do not possess a TOR nutrient-sensing pathway and have only a rudimentary amino acid starvation-sensing eukaryotic initiation factor 2α (eIF2α) stress response. Isoleucine deprivation results in GCN2-mediated phosphorylation of eIF2α, but kinase-knockout clones still are able to hibernate and recover, indicating that this pathway does not directly promote survival during isoleucine starvation. We conclude that P. falciparum, in the absence of canonical eukaryotic nutrient stress-response pathways, can cope with an inconsistent bloodstream amino acid supply by hibernating and waiting for more nutrient to be provided.

Original languageEnglish (US)
Pages (from-to)E3278-E3287
JournalProceedings of the National Academy of Sciences of the United States of America
Volume109
Issue number47
DOIs
StatePublished - Nov 20 2012

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Plasmodium falciparum
Starvation
Isoleucine
Parasites
Amino Acids
Eukaryotic Initiation Factor-2
Food
Hemoglobins
Plasmodium
Falciparum Malaria
Microarray Analysis
Proteolysis
Culture Media
Phosphotransferases
Clone Cells
Erythrocytes
Maintenance
Phosphorylation
Survival
Growth

All Science Journal Classification (ASJC) codes

  • General

Cite this

Babbitt, S. E., Altenhofen, L., Cobbold, S. A., Istvan, E. S., Fennellf, C., Doerig, C., ... Goldberg, D. E. (2012). Plasmodium falciparum responds to amino acid starvation by entering into a hibernatory state. Proceedings of the National Academy of Sciences of the United States of America, 109(47), E3278-E3287. https://doi.org/10.1073/pnas.1209823109
Babbitt, Shalon E. ; Altenhofen, Lindsey ; Cobbold, Simon A. ; Istvan, Eva S. ; Fennellf, Clare ; Doerig, Christian ; Llinás, Manuel ; Goldberg, Daniel E. / Plasmodium falciparum responds to amino acid starvation by entering into a hibernatory state. In: Proceedings of the National Academy of Sciences of the United States of America. 2012 ; Vol. 109, No. 47. pp. E3278-E3287.
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Plasmodium falciparum responds to amino acid starvation by entering into a hibernatory state. / Babbitt, Shalon E.; Altenhofen, Lindsey; Cobbold, Simon A.; Istvan, Eva S.; Fennellf, Clare; Doerig, Christian; Llinás, Manuel; Goldberg, Daniel E.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 109, No. 47, 20.11.2012, p. E3278-E3287.

Research output: Contribution to journalArticle

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AU - Babbitt, Shalon E.

AU - Altenhofen, Lindsey

AU - Cobbold, Simon A.

AU - Istvan, Eva S.

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AU - Llinás, Manuel

AU - Goldberg, Daniel E.

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AB - The human malaria parasite Plasmodium falciparum is auxotrophic for most amino acids. Its amino acid needs are met largely through the degradation of host erythrocyte hemoglobin; however the parasite must acquire isoleucine exogenously, because this amino acid is not present in adult human hemoglobin. We report that when isoleucine is withdrawn from the culture medium of intraerythrocytic P. falciparum, the parasite slows its metabolism and progresses through its developmental cycle at a reduced rate. Isoleucine- starved parasites remain viable for 72 h and resume rapid growth upon resupplementation. Protein degradation during starvation is important for maintenance of this hibernatory state. Microarray analysis of starved parasites revealed a 60% decrease in the rate of progression through the normal transcriptional program but no other apparent stress response. Plasmodium parasites do not possess a TOR nutrient-sensing pathway and have only a rudimentary amino acid starvation-sensing eukaryotic initiation factor 2α (eIF2α) stress response. Isoleucine deprivation results in GCN2-mediated phosphorylation of eIF2α, but kinase-knockout clones still are able to hibernate and recover, indicating that this pathway does not directly promote survival during isoleucine starvation. We conclude that P. falciparum, in the absence of canonical eukaryotic nutrient stress-response pathways, can cope with an inconsistent bloodstream amino acid supply by hibernating and waiting for more nutrient to be provided.

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