Plasmodium vivax liver stage development and hypnozoite persistence in human liver-chimeric mice

Sebastian A. Mikolajczak, Ashley M. Vaughan, Niwat Kangwanrangsan, Wanlapa Roobsoong, Matthew Fishbaugher, Narathatai Yimamnuaychok, Nastaran Rezakhani, Viswanathan Lakshmanan, Naresh Singh, Alexis Kaushansky, Nelly Camargo, Michael Baldwin, Scott E. Lindner, John H. Adams, Jetsumon Sattabongkot, Stefan H.I. Kappe

Research output: Contribution to journalArticle

90 Scopus citations

Abstract

Plasmodium vivax malaria is characterized by periodic relapses of symptomatic blood stage parasite infections likely initiated by activation of dormant liver stage parasites-hypnozoites. The lack of tractable P. vivax animal models constitutes an obstacle in examining P. vivax liver stage infection and drug efficacy. To overcome this obstacle, we have used human liver-chimeric (huHep) FRG KO mice as a model for P. vivax infection. FRG KO huHep mice support P. vivax sporozoite infection, liver stage development, and hypnozoite formation. We show complete P. vivax liver stage development, including maturation into infectious exo-erythrocytic merozoites as well as the formation and persistence of hypnozoites. Prophylaxis or treatment with the antimalarial primaquine can prevent and eliminate liver stage infection, respectively. Thus, P. vivax-infected FRG KO huHep mice are a model to investigate liver stage development and dormancy and may facilitate the discovery of drugs targeting relapsing malaria.

Original languageEnglish (US)
Pages (from-to)526-535
Number of pages10
JournalCell Host and Microbe
Volume17
Issue number4
DOIs
StatePublished - Apr 8 2015

    Fingerprint

All Science Journal Classification (ASJC) codes

  • Parasitology
  • Microbiology
  • Virology

Cite this

Mikolajczak, S. A., Vaughan, A. M., Kangwanrangsan, N., Roobsoong, W., Fishbaugher, M., Yimamnuaychok, N., Rezakhani, N., Lakshmanan, V., Singh, N., Kaushansky, A., Camargo, N., Baldwin, M., Lindner, S. E., Adams, J. H., Sattabongkot, J., & Kappe, S. H. I. (2015). Plasmodium vivax liver stage development and hypnozoite persistence in human liver-chimeric mice. Cell Host and Microbe, 17(4), 526-535. https://doi.org/10.1016/j.chom.2015.02.011