Plasticity in exocytosis revealed through the effects of repetitive stimuli affect the content of nanometer vesicles and the fraction of transmitter released

Chaoyi Gu, Anna Larsson, Andrew G. Ewing

Research output: Contribution to journalArticlepeer-review

Abstract

Electrochemical techniques with disk and nano-tip electrodes, together with calcium imaging, were used to examine the effect of short-interval repetitive stimuli on both exocytosis and vesicular content in a model cell line. We show that the number of events decreases markedly with repeated stimuli suggesting a depletion of exocytosis machinery. However, repetitive stimuli induce a more stable fusion pore, leading to an increased amount of neurotransmitter release. In contrast, the total neurotransmitter content inside the vesicles decreases after repetitive stimuli, resulting in a higher average release fraction from each event. We suggest a possible mechanism regarding a link between activity-induced plasticity and fraction of release.

Original languageEnglish (US)
Pages (from-to)21409-21415
Number of pages7
JournalProceedings of the National Academy of Sciences of the United States of America
Volume116
Issue number43
DOIs
StatePublished - Oct 22 2019

All Science Journal Classification (ASJC) codes

  • General

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