We have demonstrated that inhibition of NO synthase (NOS) in endothelial cells by either the NOS inhibitor Nω-monomethyl-L-arginine (L-NMMA) or the internalization of caveolin-1 scaffolding domain attenuated platelet-activating factor (PAF)-induced increases in microvessel permeability (Am J Physiol Heart Circ Physiol 286: H195-H201, 2004) indicating the involvement of an NO-dependent signaling pathway. To investigate whether an increase in endothelial cytoplasmic Ca2+ concentration ([Ca 2+]i) is the initiating event and Ca2+- dependent NO production is crucial for permeability increases, PAF (10 nM)-induced changes in endothelial [Ca2+]i and NO production were measured in individually perfused rat mesenteric venular microvessels via fluorescence microscopy. When venular microvessels were exposed to PAF, endothelial [Ca2+]i increased from 69 ± 8 nM to a peak value of 374 ± 26 nM within 3 min and then declined to a sustained level at 190 ± 12 nM after 15 min. Inhibition of NOS did not modify PAF-induced increases in endothelial [Ca2+]i. PAF-induced NO production was visualized and quantified at cellular levels in individually perfused microvessels using 4,5-diaminofluorescein diacetate and fluorescence imaging. Increased fluorescence intensity (FI), which is an indication of increased NO production, occurred in 75 ± 7% of endothelial cells in each vessel. The mean maximum FI increase was 140 ± 7% of baseline value. This increased FI was abolished by pretreatment of the vessel with L-NMMA and attenuated in the absence of extracellular Ca2+. These results provide direct evidence from intact microvessels that increased endothelial [Ca2+]i is the initial signal that activates endothelial NOS, and the subsequent increased NO production contributes to PAF-induced increases in microvessel permeability.
|Original language||English (US)|
|Journal||American Journal of Physiology - Heart and Circulatory Physiology|
|Issue number||6 57-6|
|State||Published - Jun 1 2005|
All Science Journal Classification (ASJC) codes
- Cardiology and Cardiovascular Medicine
- Physiology (medical)