Platelet-activating factor-induced increases in glucose kinetics

C. H. Lang, C. Dobrescu, D. M. Hargrove, G. J. Bagby, J. J. Spitzer

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

Platelet-activating factor (PAF) is a postulated mediator of many of the early hemodynamic effects of endotoxin. The aim of the present study was to determine whether in vivo administration of PAF could produce alterations in whole-body glucose metabolism that would mimic those seen during endotoxemia. Glucose kinetics were assessed in chronically catheterized conscious rats by the constant infusion of [6-3H]- and [U-14C]glucose before and for 4 h after either a bolus injection (300 ng/kg) or a constant infusion (20 or 220 ng·min-1·kg-1) of PAF. The bolus injection of PAF produced a 30% decrease in blood pressure by 5 min that returned to control levels by 30 min. Increased plasma glucose (40%) and lactate (150%) levels after injection of PAF were also transient. In contrast, the bolus injection of PAF elevated the rate of glucose appearance (R(a); 44%) for 1.5 h. The lower PAF infusion rate decreased blood pressure 11% to 104 mmHg, whereas the higher infusion rate decreased pressure 34% to 77 mmHg. Both PAF infusion rates produced elevations in plasma glucose (28, 150%) and glucose R(a) (20, 60%) throughout the 4-h infusion period in a dose-related manner. The PAF infusions also induced dose-related increases in plasma glucagon and catecholamine levels throughout the infusion period. In a separate group of experiments a complete adrenergic blockade, produced by the constant infusion of propranolol and phentolamine, completely prevented PAF-induced increases in glucose kinetics and the hyperglucagonemia. Because the constant infusion of PAF did simulate many of the hemodynamic and metabolic alterations produced by endotoxin, this study provides additional support for the potential importance of PAF as a mediator of the early hemodynamic and metabolic sequela of endotoxin shock. Furthermore, the PAF-induced changes in glucose metabolism appear to be mediated by the resultant elevation in plasma catecholamines.

Original languageEnglish (US)
JournalAmerican Journal of Physiology - Endocrinology and Metabolism
Volume254
Issue number2
StatePublished - Jan 1 1988

Fingerprint

Platelet Activating Factor
Glucose
Endotoxins
Injections
Hemodynamics
Catecholamines
Blood Pressure
Endotoxemia
Phentolamine
Glucagon
Propranolol
Adrenergic Agents
Shock
Lactic Acid

All Science Journal Classification (ASJC) codes

  • Endocrinology, Diabetes and Metabolism
  • Physiology
  • Physiology (medical)

Cite this

Lang, C. H. ; Dobrescu, C. ; Hargrove, D. M. ; Bagby, G. J. ; Spitzer, J. J. / Platelet-activating factor-induced increases in glucose kinetics. In: American Journal of Physiology - Endocrinology and Metabolism. 1988 ; Vol. 254, No. 2.
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abstract = "Platelet-activating factor (PAF) is a postulated mediator of many of the early hemodynamic effects of endotoxin. The aim of the present study was to determine whether in vivo administration of PAF could produce alterations in whole-body glucose metabolism that would mimic those seen during endotoxemia. Glucose kinetics were assessed in chronically catheterized conscious rats by the constant infusion of [6-3H]- and [U-14C]glucose before and for 4 h after either a bolus injection (300 ng/kg) or a constant infusion (20 or 220 ng·min-1·kg-1) of PAF. The bolus injection of PAF produced a 30{\%} decrease in blood pressure by 5 min that returned to control levels by 30 min. Increased plasma glucose (40{\%}) and lactate (150{\%}) levels after injection of PAF were also transient. In contrast, the bolus injection of PAF elevated the rate of glucose appearance (R(a); 44{\%}) for 1.5 h. The lower PAF infusion rate decreased blood pressure 11{\%} to 104 mmHg, whereas the higher infusion rate decreased pressure 34{\%} to 77 mmHg. Both PAF infusion rates produced elevations in plasma glucose (28, 150{\%}) and glucose R(a) (20, 60{\%}) throughout the 4-h infusion period in a dose-related manner. The PAF infusions also induced dose-related increases in plasma glucagon and catecholamine levels throughout the infusion period. In a separate group of experiments a complete adrenergic blockade, produced by the constant infusion of propranolol and phentolamine, completely prevented PAF-induced increases in glucose kinetics and the hyperglucagonemia. Because the constant infusion of PAF did simulate many of the hemodynamic and metabolic alterations produced by endotoxin, this study provides additional support for the potential importance of PAF as a mediator of the early hemodynamic and metabolic sequela of endotoxin shock. Furthermore, the PAF-induced changes in glucose metabolism appear to be mediated by the resultant elevation in plasma catecholamines.",
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Platelet-activating factor-induced increases in glucose kinetics. / Lang, C. H.; Dobrescu, C.; Hargrove, D. M.; Bagby, G. J.; Spitzer, J. J.

In: American Journal of Physiology - Endocrinology and Metabolism, Vol. 254, No. 2, 01.01.1988.

Research output: Contribution to journalArticle

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