Platelet-activating factor may act as an endogenous pulse generator for sheep of luteolytic PGF(2α) release

O. Chami, A. Megevand, Troy Ott, F. Bazer, C. O'Neill

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Pulsatile release of uterine prostaglandin F(2α), (PGF(2α)) induces luteolysis in ruminants. However, the mechanism(s) that initiates and maintains luteolysis has not been defined. The present study tested the hypothesis that the endogenous PGF(2α) pulse generator is uterine-derived platelet-activating factor (PAF). Ovariectomized ewes were given exogenous progesterone (P), estradiol (E), or both (P+E, mimicking the normal luteal phase). Only ewes treated with steroids released PAF into the uterine lumen and had increased PAF: acetylhydrolase activity in the uterine lumen. Steroid treatment also influenced the capacity of the uterus to release PGF(2α) in response to exogenous PAF. PAF infusion did not affect plasma PGF(2α) metabolite (PGFM) levels in control (no steroid treatment) ewes but increased plasma PGFM levels in P+E ewes (P < 0.001) and ewes treated with P or E alone (P < 0.05). Infusion of PAF followed by or coincident with oxytocin (OT) acted in a synergistic manner to increase plasma PGFM levels. Repeated infusion of PAF into the uterus at 1-h intervals induced tachyphylaxis of the PGFM response to PAF; however, sensitivity of the uterus to PAF returned spontaneously by the 6th h. Interferon-τ (IFN-τ) inhibits pulsatile release of PGF(2α), during pregnancy to prevent luteolysis. Exogenous recombinant ovine IFN-τ (50 μg) inhibited the uterine response to PAF alone or the combined effects of PAF and OT. These results indicate that uterine PAF fulfills many of the criteria for an endogenous PGF(2α) pulse-generator: steroid induction of PAF production and uterine responsiveness to PAF- induced release of PGF; synergistic stimulation of PAF-induced PGF release by OT; inhibition of PAF effects by IFN-τ; and PAF's ability to induce pulses of PGF with a periodicity during a period of chronic exposure of the uterus to PAF.

Original languageEnglish (US)
JournalAmerican Journal of Physiology - Endocrinology and Metabolism
Volume276
Issue number4 39-4
StatePublished - Jan 1 1999

Fingerprint

Platelet Activating Factor
Prostaglandins F
Sheep
Luteolysis
Uterus
Oxytocin
Steroids
Interferons
Tachyphylaxis
Luteal Phase
Ruminants
Periodicity

All Science Journal Classification (ASJC) codes

  • Endocrinology, Diabetes and Metabolism
  • Physiology
  • Physiology (medical)

Cite this

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title = "Platelet-activating factor may act as an endogenous pulse generator for sheep of luteolytic PGF(2α) release",
abstract = "Pulsatile release of uterine prostaglandin F(2α), (PGF(2α)) induces luteolysis in ruminants. However, the mechanism(s) that initiates and maintains luteolysis has not been defined. The present study tested the hypothesis that the endogenous PGF(2α) pulse generator is uterine-derived platelet-activating factor (PAF). Ovariectomized ewes were given exogenous progesterone (P), estradiol (E), or both (P+E, mimicking the normal luteal phase). Only ewes treated with steroids released PAF into the uterine lumen and had increased PAF: acetylhydrolase activity in the uterine lumen. Steroid treatment also influenced the capacity of the uterus to release PGF(2α) in response to exogenous PAF. PAF infusion did not affect plasma PGF(2α) metabolite (PGFM) levels in control (no steroid treatment) ewes but increased plasma PGFM levels in P+E ewes (P < 0.001) and ewes treated with P or E alone (P < 0.05). Infusion of PAF followed by or coincident with oxytocin (OT) acted in a synergistic manner to increase plasma PGFM levels. Repeated infusion of PAF into the uterus at 1-h intervals induced tachyphylaxis of the PGFM response to PAF; however, sensitivity of the uterus to PAF returned spontaneously by the 6th h. Interferon-τ (IFN-τ) inhibits pulsatile release of PGF(2α), during pregnancy to prevent luteolysis. Exogenous recombinant ovine IFN-τ (50 μg) inhibited the uterine response to PAF alone or the combined effects of PAF and OT. These results indicate that uterine PAF fulfills many of the criteria for an endogenous PGF(2α) pulse-generator: steroid induction of PAF production and uterine responsiveness to PAF- induced release of PGF; synergistic stimulation of PAF-induced PGF release by OT; inhibition of PAF effects by IFN-τ; and PAF's ability to induce pulses of PGF with a periodicity during a period of chronic exposure of the uterus to PAF.",
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Platelet-activating factor may act as an endogenous pulse generator for sheep of luteolytic PGF(2α) release. / Chami, O.; Megevand, A.; Ott, Troy; Bazer, F.; O'Neill, C.

In: American Journal of Physiology - Endocrinology and Metabolism, Vol. 276, No. 4 39-4, 01.01.1999.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Platelet-activating factor may act as an endogenous pulse generator for sheep of luteolytic PGF(2α) release

AU - Chami, O.

AU - Megevand, A.

AU - Ott, Troy

AU - Bazer, F.

AU - O'Neill, C.

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N2 - Pulsatile release of uterine prostaglandin F(2α), (PGF(2α)) induces luteolysis in ruminants. However, the mechanism(s) that initiates and maintains luteolysis has not been defined. The present study tested the hypothesis that the endogenous PGF(2α) pulse generator is uterine-derived platelet-activating factor (PAF). Ovariectomized ewes were given exogenous progesterone (P), estradiol (E), or both (P+E, mimicking the normal luteal phase). Only ewes treated with steroids released PAF into the uterine lumen and had increased PAF: acetylhydrolase activity in the uterine lumen. Steroid treatment also influenced the capacity of the uterus to release PGF(2α) in response to exogenous PAF. PAF infusion did not affect plasma PGF(2α) metabolite (PGFM) levels in control (no steroid treatment) ewes but increased plasma PGFM levels in P+E ewes (P < 0.001) and ewes treated with P or E alone (P < 0.05). Infusion of PAF followed by or coincident with oxytocin (OT) acted in a synergistic manner to increase plasma PGFM levels. Repeated infusion of PAF into the uterus at 1-h intervals induced tachyphylaxis of the PGFM response to PAF; however, sensitivity of the uterus to PAF returned spontaneously by the 6th h. Interferon-τ (IFN-τ) inhibits pulsatile release of PGF(2α), during pregnancy to prevent luteolysis. Exogenous recombinant ovine IFN-τ (50 μg) inhibited the uterine response to PAF alone or the combined effects of PAF and OT. These results indicate that uterine PAF fulfills many of the criteria for an endogenous PGF(2α) pulse-generator: steroid induction of PAF production and uterine responsiveness to PAF- induced release of PGF; synergistic stimulation of PAF-induced PGF release by OT; inhibition of PAF effects by IFN-τ; and PAF's ability to induce pulses of PGF with a periodicity during a period of chronic exposure of the uterus to PAF.

AB - Pulsatile release of uterine prostaglandin F(2α), (PGF(2α)) induces luteolysis in ruminants. However, the mechanism(s) that initiates and maintains luteolysis has not been defined. The present study tested the hypothesis that the endogenous PGF(2α) pulse generator is uterine-derived platelet-activating factor (PAF). Ovariectomized ewes were given exogenous progesterone (P), estradiol (E), or both (P+E, mimicking the normal luteal phase). Only ewes treated with steroids released PAF into the uterine lumen and had increased PAF: acetylhydrolase activity in the uterine lumen. Steroid treatment also influenced the capacity of the uterus to release PGF(2α) in response to exogenous PAF. PAF infusion did not affect plasma PGF(2α) metabolite (PGFM) levels in control (no steroid treatment) ewes but increased plasma PGFM levels in P+E ewes (P < 0.001) and ewes treated with P or E alone (P < 0.05). Infusion of PAF followed by or coincident with oxytocin (OT) acted in a synergistic manner to increase plasma PGFM levels. Repeated infusion of PAF into the uterus at 1-h intervals induced tachyphylaxis of the PGFM response to PAF; however, sensitivity of the uterus to PAF returned spontaneously by the 6th h. Interferon-τ (IFN-τ) inhibits pulsatile release of PGF(2α), during pregnancy to prevent luteolysis. Exogenous recombinant ovine IFN-τ (50 μg) inhibited the uterine response to PAF alone or the combined effects of PAF and OT. These results indicate that uterine PAF fulfills many of the criteria for an endogenous PGF(2α) pulse-generator: steroid induction of PAF production and uterine responsiveness to PAF- induced release of PGF; synergistic stimulation of PAF-induced PGF release by OT; inhibition of PAF effects by IFN-τ; and PAF's ability to induce pulses of PGF with a periodicity during a period of chronic exposure of the uterus to PAF.

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