Platelet adhesion to polyurethane urea under pulsatile flow conditions

Michael A. Navitsky, Joshua O. Taylor, Alexander B. Smith, Margaret J. Slattery, Steven Deutsch, Christopher A. Siedlecki, Keefe B. Manning

Research output: Contribution to journalArticle

11 Scopus citations

Abstract

Platelet adhesion to a polyurethane urea surface is a precursor to thrombus formation within blood-contacting cardiovascular devices, and platelets have been found to adhere strongly to polyurethane surfaces below a shear rate of approximately 500s-1. The aim of the current work is to determine the properties of platelet adhesion to the polyurethane urea surface as a function of time-varying shear exposure. A rotating disk system was used to study the influence of steady and pulsatile flow conditions (e.g., cardiac inflow and sawtooth waveforms) for platelet adhesion to the biomaterial surface. All experiments were conducted with the same root mean square angular rotation velocity (29.63rad/s) and waveform period. The disk was rotated in platelet-rich bovine plasma for 2h, with adhesion quantified by confocal microscopy measurements of immunofluorescently labeled bovine platelets. Platelet adhesion under pulsating flow was found to decay exponentially with increasing shear rate. Adhesion levels were found to depend upon peak platelet flux and shear rate, regardless of rotational waveform. In combination with flow measurements, these results may be useful for predicting regions susceptible to thrombus formation within ventricular assist devices.

Original languageEnglish (US)
Pages (from-to)1046-1053
Number of pages8
JournalArtificial organs
Volume38
Issue number12
DOIs
Publication statusPublished - Dec 1 2014

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All Science Journal Classification (ASJC) codes

  • Bioengineering
  • Medicine (miscellaneous)
  • Biomaterials
  • Biomedical Engineering

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