Pleurotus eryngii species complex

Sequence analysis and phylogeny based on partial EF1α and RPB2 genes

A. E R Estrada, Maria Del Mar Jimenez Gasco, Daniel J. Royse

Research output: Contribution to journalArticle

29 Citations (Scopus)

Abstract

The Pleurotus eryngii species complex comprises at least six varieties (var. eryngii (DC.: Fr) Quel., ferulae Lanzi, elaeoselini Venturella et al., nebrodensis (Inzenga) Sacc., tingitanus Lewinsohn et al. and tuoliensis C.J. Mou). This species is unique among the genus Pleurotus because in nature it is found in association with certain species of the Apiaceae (Umbelliferae) and Asteraceae (Compositae) families. Sequences of partial regions of the translation elongation factor (EF1α) and RNA polymerase II (RPB2) genes were analyzed in order to detect nucleotide polymorphisms that might unequivocally distinguish varieties eryngii, ferulae, elaeoselini and nebrodensis. A phylogenetic analysis was also performed with an aim to establish phylogenetic relationships among those. Sequence analysis of the partial EF1α and RPB2 genes contained nucleotide polymorphisms able to unequivocally distinguish variety nebrodensis from the rest. However, distinction among eryngii, elaeoselini and ferulae was achieved only through the RPB2 gene. The phylogenetic analyses from the combined data sets (EF1α and RPB2) indicated that P. eryngii is a monophyletic group and that varieties eryngii, elaeoselini and ferulae are closely related. P. eryngii var. nebrodensis was placed in a distinct clade clearly differentiated from the other varieties but still monophyletic with the P. eryngii complex. The limited nucleotide variation in partial EF1α and RPB2 among varieties eryngii, ferulae and elaeoselini supports the placement of these groups as varieties and not species within the complex.

Original languageEnglish (US)
Pages (from-to)421-428
Number of pages8
JournalFungal Biology
Volume114
Issue number5-6
DOIs
StatePublished - May 1 2010

Fingerprint

Pleurotus eryngii
Pleurotus
species complex
Phylogeny
Apiaceae
Sequence Analysis
Asteraceae
phylogeny
Nucleotides
sequence analysis
phylogenetics
gene
polymorphism
Genes
Peptide Elongation Factors
genes
RNA Polymerase II
nucleotides
RNA
genetic polymorphism

All Science Journal Classification (ASJC) codes

  • Ecology, Evolution, Behavior and Systematics
  • Plant Science
  • Genetics
  • Infectious Diseases

Cite this

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title = "Pleurotus eryngii species complex: Sequence analysis and phylogeny based on partial EF1α and RPB2 genes",
abstract = "The Pleurotus eryngii species complex comprises at least six varieties (var. eryngii (DC.: Fr) Quel., ferulae Lanzi, elaeoselini Venturella et al., nebrodensis (Inzenga) Sacc., tingitanus Lewinsohn et al. and tuoliensis C.J. Mou). This species is unique among the genus Pleurotus because in nature it is found in association with certain species of the Apiaceae (Umbelliferae) and Asteraceae (Compositae) families. Sequences of partial regions of the translation elongation factor (EF1α) and RNA polymerase II (RPB2) genes were analyzed in order to detect nucleotide polymorphisms that might unequivocally distinguish varieties eryngii, ferulae, elaeoselini and nebrodensis. A phylogenetic analysis was also performed with an aim to establish phylogenetic relationships among those. Sequence analysis of the partial EF1α and RPB2 genes contained nucleotide polymorphisms able to unequivocally distinguish variety nebrodensis from the rest. However, distinction among eryngii, elaeoselini and ferulae was achieved only through the RPB2 gene. The phylogenetic analyses from the combined data sets (EF1α and RPB2) indicated that P. eryngii is a monophyletic group and that varieties eryngii, elaeoselini and ferulae are closely related. P. eryngii var. nebrodensis was placed in a distinct clade clearly differentiated from the other varieties but still monophyletic with the P. eryngii complex. The limited nucleotide variation in partial EF1α and RPB2 among varieties eryngii, ferulae and elaeoselini supports the placement of these groups as varieties and not species within the complex.",
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Pleurotus eryngii species complex : Sequence analysis and phylogeny based on partial EF1α and RPB2 genes. / Estrada, A. E R; Jimenez Gasco, Maria Del Mar; Royse, Daniel J.

In: Fungal Biology, Vol. 114, No. 5-6, 01.05.2010, p. 421-428.

Research output: Contribution to journalArticle

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