Poliovirus infection transiently increases COPII vesicle budding

Meg Trahey, Hyung Suk Oh, Craig E. Cameron, Jesse C. Hay

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

Poliovirus (PV) requires membranes of the host cell's secretory pathway to generate replication complexes (RCs) for viral RNA synthesis. Recent work identified the intermediate compartment and the Golgi apparatus as the precursors of the replication "organelles" of PV (N. Y. Hsu et al., Cell 141:799-811, 2010). In this study, we examined the effect of PV on COPII vesicles, the secretory cargo carriers that bud from the endoplasmic reticulum and homotypically fuse to form the intermediate compartment that matures into the Golgi apparatus. We found that infection by PV results in a biphasic change in functional COPII vesicle biogenesis in cells, with an early enhancement and subsequent inhibition. Concomitant with the early increase in COPII vesicle formation, we found an increase in the membrane fraction of Sec16A, a key regulator of COPII vesicle formation. We suggest that the early burst in COPII vesicle formation detected benefits PV by increasing the precursor pool required for the formation of its RCs.

Original languageEnglish (US)
Pages (from-to)9675-9682
Number of pages8
JournalJournal of virology
Volume86
Issue number18
DOIs
StatePublished - Sep 1 2012

Fingerprint

Enterovirus C
Poliovirus
Infection
infection
Golgi Apparatus
Golgi apparatus
Secretory Pathway
secretory granules
Secretory Vesicles
Viral RNA
cells
Endoplasmic Reticulum
Organelles
endoplasmic reticulum
organelles
buds
Cell Membrane
RNA
synthesis
Membranes

All Science Journal Classification (ASJC) codes

  • Microbiology
  • Immunology
  • Insect Science
  • Virology

Cite this

Trahey, M., Oh, H. S., Cameron, C. E., & Hay, J. C. (2012). Poliovirus infection transiently increases COPII vesicle budding. Journal of virology, 86(18), 9675-9682. https://doi.org/10.1128/JVI.01159-12
Trahey, Meg ; Oh, Hyung Suk ; Cameron, Craig E. ; Hay, Jesse C. / Poliovirus infection transiently increases COPII vesicle budding. In: Journal of virology. 2012 ; Vol. 86, No. 18. pp. 9675-9682.
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Trahey, M, Oh, HS, Cameron, CE & Hay, JC 2012, 'Poliovirus infection transiently increases COPII vesicle budding', Journal of virology, vol. 86, no. 18, pp. 9675-9682. https://doi.org/10.1128/JVI.01159-12

Poliovirus infection transiently increases COPII vesicle budding. / Trahey, Meg; Oh, Hyung Suk; Cameron, Craig E.; Hay, Jesse C.

In: Journal of virology, Vol. 86, No. 18, 01.09.2012, p. 9675-9682.

Research output: Contribution to journalArticle

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