Pollen fertility restoration by nuclear gene Fr in CMS common bean

an Fr linkage map and the mode of Fr action

S. He, Z. H. Yu, C. E. Vallejos, Sally Mackenzie

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

The Fr gene in common bean, Phaseolus vulgaris L., is a unique gene for the study of plant nuclear-mitochondrial interactions because it appears to directly influence plant mitochondrial genome structure, resulting in the restoration of pollen fertility in cytoplasmic male sterile plants. This gene action is distinct from other pollen fertility restoration systems characterized to date. As a first step towards the map-based cloning of this unusual nuclear gene, we identified RAPD markers linked to Fr using bulked segregant analysis of near-isogenic lines. Using DNA gel blot hybridization, we localized the identified RAPD markers to a linkage group on the common bean RFLP map and constructed a linkage map of the Fr region using both RAPD markers and RFLP markers. Analysis of the mode of Fr action with the aid of identified Fr-linked DNA markers indicated that Fr functions in a semidominant fashion, showing dosage effect in controlling the dynamics of a heteroplasmic mitochondrial population. We also present our observations on the developmental distinctions, crucial in the accurate mapping of the Fr gene, between spontaneous cytoplasmic reversion and Fr-driven fertility restoration, two phenomena that are phenotypically indistinguishable.

Original languageEnglish (US)
Pages (from-to)1056-1062
Number of pages7
JournalTheoretical and Applied Genetics
Volume90
Issue number7-8
DOIs
StatePublished - Jun 1 1995

Fingerprint

Pollen
chromosome mapping
Fertility
mechanism of action
beans
pollen
Restriction Fragment Length Polymorphisms
Genes
Plant Genome
Plant Genes
restriction fragment length polymorphism
Mitochondrial Genome
Phaseolus
genes
nuclear-cytoplasmic interactions
Chromosome Mapping
Genetic Markers
Organism Cloning
isogenic lines
Gels

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Agronomy and Crop Science
  • Genetics

Cite this

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title = "Pollen fertility restoration by nuclear gene Fr in CMS common bean: an Fr linkage map and the mode of Fr action",
abstract = "The Fr gene in common bean, Phaseolus vulgaris L., is a unique gene for the study of plant nuclear-mitochondrial interactions because it appears to directly influence plant mitochondrial genome structure, resulting in the restoration of pollen fertility in cytoplasmic male sterile plants. This gene action is distinct from other pollen fertility restoration systems characterized to date. As a first step towards the map-based cloning of this unusual nuclear gene, we identified RAPD markers linked to Fr using bulked segregant analysis of near-isogenic lines. Using DNA gel blot hybridization, we localized the identified RAPD markers to a linkage group on the common bean RFLP map and constructed a linkage map of the Fr region using both RAPD markers and RFLP markers. Analysis of the mode of Fr action with the aid of identified Fr-linked DNA markers indicated that Fr functions in a semidominant fashion, showing dosage effect in controlling the dynamics of a heteroplasmic mitochondrial population. We also present our observations on the developmental distinctions, crucial in the accurate mapping of the Fr gene, between spontaneous cytoplasmic reversion and Fr-driven fertility restoration, two phenomena that are phenotypically indistinguishable.",
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Pollen fertility restoration by nuclear gene Fr in CMS common bean : an Fr linkage map and the mode of Fr action. / He, S.; Yu, Z. H.; Vallejos, C. E.; Mackenzie, Sally.

In: Theoretical and Applied Genetics, Vol. 90, No. 7-8, 01.06.1995, p. 1056-1062.

Research output: Contribution to journalArticle

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