Pollen gene flow and molecular identification of full-sib families in small and isolated population fragments of Gleditsia triacanthos L

Sandra A. Owusu, Scott E. Schlarbaum, John Edward Carlson, Oliver Gailing

Research output: Contribution to journalArticle

5 Scopus citations

Abstract

To analyze the utility of isolated remnant populations for full-sibling (full-sib) identification among openpollinated single-tree progeny in the outcrossing and insect-pollinated tree Gleditsia triacanthos L. (honey locust), we performed paternity analyses in forest fragments from two geographic regions using nuclear microsatellites. The first plot (Butternut Valley population) comprised only 7 trees, and 552 seedlings from a single seed parent were characterized at nuclear microsatellites. A large number of putative pollen donors (59) were identified in kinship analyses, but their individual contributions to the progeny were highly variable. Kinship and paternity analyses identified 149 putative full-sibs for genetic mapping sired by an external (unsampled) pollen parent. To better assess the frequency of long-distance pollen dispersal, a total of 180 seeds were collected from 6 seed parents in another fragmented population. In both plots, contemporary pollen dispersal occurred generally from outside the plots (99.38% and 87.50%–100% at the Butternut Valley and Ames Plantation sites, respectively) and thus over very long distances (>12 000min the Ames Plantation) suggesting that in highly fragmented landscapes, insect pollinators of honey locust are likely very effective long-distance dispersers.

Original languageEnglish (US)
Pages (from-to)523-532
Number of pages10
JournalBotany
Volume94
Issue number7
DOIs
StatePublished - Mar 28 2016

All Science Journal Classification (ASJC) codes

  • Ecology, Evolution, Behavior and Systematics
  • Ecology
  • Plant Science

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