Pollen gene flow and molecular identification of full-sib families in small and isolated population fragments of Gleditsia triacanthos L

Sandra A. Owusu, Scott E. Schlarbaum, John Edward Carlson, Oliver Gailing

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

To analyze the utility of isolated remnant populations for full-sibling (full-sib) identification among openpollinated single-tree progeny in the outcrossing and insect-pollinated tree Gleditsia triacanthos L. (honey locust), we performed paternity analyses in forest fragments from two geographic regions using nuclear microsatellites. The first plot (Butternut Valley population) comprised only 7 trees, and 552 seedlings from a single seed parent were characterized at nuclear microsatellites. A large number of putative pollen donors (59) were identified in kinship analyses, but their individual contributions to the progeny were highly variable. Kinship and paternity analyses identified 149 putative full-sibs for genetic mapping sired by an external (unsampled) pollen parent. To better assess the frequency of long-distance pollen dispersal, a total of 180 seeds were collected from 6 seed parents in another fragmented population. In both plots, contemporary pollen dispersal occurred generally from outside the plots (99.38% and 87.50%–100% at the Butternut Valley and Ames Plantation sites, respectively) and thus over very long distances (>12 000min the Ames Plantation) suggesting that in highly fragmented landscapes, insect pollinators of honey locust are likely very effective long-distance dispersers.

Original languageEnglish (US)
Pages (from-to)523-532
Number of pages10
JournalBotany
Volume94
Issue number7
DOIs
StatePublished - Mar 28 2016

Fingerprint

Gleditsia triacanthos
isolated population
gene flow
pollen flow
pollen
kinship
paternity
locust
honey
valleys
plantations
seeds
seed
microsatellite repeats
plantation
pollinating insects
insect
outcrossing
valley
habitat fragmentation

All Science Journal Classification (ASJC) codes

  • Ecology, Evolution, Behavior and Systematics
  • Ecology
  • Plant Science

Cite this

@article{b75b8a94c5314613a24363673c6aa271,
title = "Pollen gene flow and molecular identification of full-sib families in small and isolated population fragments of Gleditsia triacanthos L",
abstract = "To analyze the utility of isolated remnant populations for full-sibling (full-sib) identification among openpollinated single-tree progeny in the outcrossing and insect-pollinated tree Gleditsia triacanthos L. (honey locust), we performed paternity analyses in forest fragments from two geographic regions using nuclear microsatellites. The first plot (Butternut Valley population) comprised only 7 trees, and 552 seedlings from a single seed parent were characterized at nuclear microsatellites. A large number of putative pollen donors (59) were identified in kinship analyses, but their individual contributions to the progeny were highly variable. Kinship and paternity analyses identified 149 putative full-sibs for genetic mapping sired by an external (unsampled) pollen parent. To better assess the frequency of long-distance pollen dispersal, a total of 180 seeds were collected from 6 seed parents in another fragmented population. In both plots, contemporary pollen dispersal occurred generally from outside the plots (99.38{\%} and 87.50{\%}–100{\%} at the Butternut Valley and Ames Plantation sites, respectively) and thus over very long distances (>12 000min the Ames Plantation) suggesting that in highly fragmented landscapes, insect pollinators of honey locust are likely very effective long-distance dispersers.",
author = "Owusu, {Sandra A.} and Schlarbaum, {Scott E.} and Carlson, {John Edward} and Oliver Gailing",
year = "2016",
month = "3",
day = "28",
doi = "10.1139/cjb-2015-0244",
language = "English (US)",
volume = "94",
pages = "523--532",
journal = "Botany",
issn = "1916-2790",
publisher = "National Research Council of Canada",
number = "7",

}

Pollen gene flow and molecular identification of full-sib families in small and isolated population fragments of Gleditsia triacanthos L. / Owusu, Sandra A.; Schlarbaum, Scott E.; Carlson, John Edward; Gailing, Oliver.

In: Botany, Vol. 94, No. 7, 28.03.2016, p. 523-532.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Pollen gene flow and molecular identification of full-sib families in small and isolated population fragments of Gleditsia triacanthos L

AU - Owusu, Sandra A.

AU - Schlarbaum, Scott E.

AU - Carlson, John Edward

AU - Gailing, Oliver

PY - 2016/3/28

Y1 - 2016/3/28

N2 - To analyze the utility of isolated remnant populations for full-sibling (full-sib) identification among openpollinated single-tree progeny in the outcrossing and insect-pollinated tree Gleditsia triacanthos L. (honey locust), we performed paternity analyses in forest fragments from two geographic regions using nuclear microsatellites. The first plot (Butternut Valley population) comprised only 7 trees, and 552 seedlings from a single seed parent were characterized at nuclear microsatellites. A large number of putative pollen donors (59) were identified in kinship analyses, but their individual contributions to the progeny were highly variable. Kinship and paternity analyses identified 149 putative full-sibs for genetic mapping sired by an external (unsampled) pollen parent. To better assess the frequency of long-distance pollen dispersal, a total of 180 seeds were collected from 6 seed parents in another fragmented population. In both plots, contemporary pollen dispersal occurred generally from outside the plots (99.38% and 87.50%–100% at the Butternut Valley and Ames Plantation sites, respectively) and thus over very long distances (>12 000min the Ames Plantation) suggesting that in highly fragmented landscapes, insect pollinators of honey locust are likely very effective long-distance dispersers.

AB - To analyze the utility of isolated remnant populations for full-sibling (full-sib) identification among openpollinated single-tree progeny in the outcrossing and insect-pollinated tree Gleditsia triacanthos L. (honey locust), we performed paternity analyses in forest fragments from two geographic regions using nuclear microsatellites. The first plot (Butternut Valley population) comprised only 7 trees, and 552 seedlings from a single seed parent were characterized at nuclear microsatellites. A large number of putative pollen donors (59) were identified in kinship analyses, but their individual contributions to the progeny were highly variable. Kinship and paternity analyses identified 149 putative full-sibs for genetic mapping sired by an external (unsampled) pollen parent. To better assess the frequency of long-distance pollen dispersal, a total of 180 seeds were collected from 6 seed parents in another fragmented population. In both plots, contemporary pollen dispersal occurred generally from outside the plots (99.38% and 87.50%–100% at the Butternut Valley and Ames Plantation sites, respectively) and thus over very long distances (>12 000min the Ames Plantation) suggesting that in highly fragmented landscapes, insect pollinators of honey locust are likely very effective long-distance dispersers.

UR - http://www.scopus.com/inward/record.url?scp=84979022641&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84979022641&partnerID=8YFLogxK

U2 - 10.1139/cjb-2015-0244

DO - 10.1139/cjb-2015-0244

M3 - Article

AN - SCOPUS:84979022641

VL - 94

SP - 523

EP - 532

JO - Botany

JF - Botany

SN - 1916-2790

IS - 7

ER -