Polyamines are needed for the differentiation of 3T3-L1 fibroblasts into adipose cells

Delia R. Bethell, Anthony E. Pegg

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When non-proliferating 3T3-L1 fibroblasts were stimulated to differentiate into adipose cells, there was a dramatic increase in the intracellular level of the polyamine, spermidine. Addition of α-difluoromethylornithine, an inhibitor of polyamine biosynthesis, depleted the cellular polyamines and prevented triglyceride accumulation and differentiation. The inhibitory effect of α-difluoromethylorinithine was completely abolished by provision of spermidine or putrescine. This suggests that polyamines are needed in the processes of differentiation as well as their established requirement for cell growth.

Original languageEnglish (US)
Pages (from-to)272-278
Number of pages7
JournalBiochemical and Biophysical Research Communications
Issue number1
Publication statusPublished - Sep 16 1981


All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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