Polyamines are needed for the differentiation of 3T3-L1 fibroblasts into adipose cells

Delia R. Bethell, Anthony Pegg

Research output: Contribution to journalArticle

49 Citations (Scopus)

Abstract

When non-proliferating 3T3-L1 fibroblasts were stimulated to differentiate into adipose cells, there was a dramatic increase in the intracellular level of the polyamine, spermidine. Addition of α-difluoromethylornithine, an inhibitor of polyamine biosynthesis, depleted the cellular polyamines and prevented triglyceride accumulation and differentiation. The inhibitory effect of α-difluoromethylorinithine was completely abolished by provision of spermidine or putrescine. This suggests that polyamines are needed in the processes of differentiation as well as their established requirement for cell growth.

Original languageEnglish (US)
Pages (from-to)272-278
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume102
Issue number1
DOIs
StatePublished - Sep 16 1981

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Polyamines
Fibroblasts
Spermidine
Eflornithine
Putrescine
Biosynthesis
Cell growth
Triglycerides
Growth

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

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Polyamines are needed for the differentiation of 3T3-L1 fibroblasts into adipose cells. / Bethell, Delia R.; Pegg, Anthony.

In: Biochemical and Biophysical Research Communications, Vol. 102, No. 1, 16.09.1981, p. 272-278.

Research output: Contribution to journalArticle

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