POLYGALACTURONASE INVOLVED IN EXPANSION3 functions in seedling development, rosette growth, and stomatal dynamics in Arabidopsis thaliana

Yue Rui, Chaowen Xiao, Hojae Yi, Baris Kandemir, James Wang, Virendra Puri, Charles T. Anderson

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

Plant cell separation and expansion require pectin degradation by endogenous pectinases such as polygalacturonases, few of which have been functionally characterized. Stomata are a unique system to study both processes because stomatal maturation involves limited separation between sister guard cells and stomatal responses require reversible guard cell elongation and contraction. However, the molecular mechanisms for how stomatal pores form and how guard cell walls facilitate dynamic stomatal responses remain poorly understood. We characterized POLYGALACTURONASE INVOLVED IN EXPANSION3 (PGX3), which is expressed in expanding tissues and guard cells. PGX3-GFP localizes to the cell wall and is enriched at sites of stomatal pore initiation in cotyledons. In seedlings, ablating or overexpressing PGX3 affects both cotyledon shape and the spacing and pore dimensions of developing stomata. In adult plants, PGX3 affects rosette size. Although stomata in true leaves display normal density and morphology when PGX3 expression is altered, loss of PGX3 prevents smooth stomatal closure, and overexpression of PGX3 accelerates stomatal opening. These phenotypes correspond with changes in pectin molecular mass and abundance that can affect wall mechanics. Together, these results demonstrate that PGX3-mediated pectin degradation affects stomatal development in cotyledons, promotes rosette expansion, and modulates guard cell mechanics in adult plants.

Original languageEnglish (US)
Pages (from-to)2413-2432
Number of pages20
JournalPlant Cell
Volume29
Issue number10
DOIs
StatePublished - Oct 1 2017

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Polygalacturonase
guard cells
polygalacturonase
Seedlings
Growth and Development
Arabidopsis
Cotyledon
Arabidopsis thaliana
seedlings
stomata
pectins
cotyledons
Mechanics
Cell Wall
mature plants
mechanics
cell walls
Cell Separation
Plant Cells
degradation

All Science Journal Classification (ASJC) codes

  • Plant Science
  • Cell Biology

Cite this

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abstract = "Plant cell separation and expansion require pectin degradation by endogenous pectinases such as polygalacturonases, few of which have been functionally characterized. Stomata are a unique system to study both processes because stomatal maturation involves limited separation between sister guard cells and stomatal responses require reversible guard cell elongation and contraction. However, the molecular mechanisms for how stomatal pores form and how guard cell walls facilitate dynamic stomatal responses remain poorly understood. We characterized POLYGALACTURONASE INVOLVED IN EXPANSION3 (PGX3), which is expressed in expanding tissues and guard cells. PGX3-GFP localizes to the cell wall and is enriched at sites of stomatal pore initiation in cotyledons. In seedlings, ablating or overexpressing PGX3 affects both cotyledon shape and the spacing and pore dimensions of developing stomata. In adult plants, PGX3 affects rosette size. Although stomata in true leaves display normal density and morphology when PGX3 expression is altered, loss of PGX3 prevents smooth stomatal closure, and overexpression of PGX3 accelerates stomatal opening. These phenotypes correspond with changes in pectin molecular mass and abundance that can affect wall mechanics. Together, these results demonstrate that PGX3-mediated pectin degradation affects stomatal development in cotyledons, promotes rosette expansion, and modulates guard cell mechanics in adult plants.",
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POLYGALACTURONASE INVOLVED IN EXPANSION3 functions in seedling development, rosette growth, and stomatal dynamics in Arabidopsis thaliana. / Rui, Yue; Xiao, Chaowen; Yi, Hojae; Kandemir, Baris; Wang, James; Puri, Virendra; Anderson, Charles T.

In: Plant Cell, Vol. 29, No. 10, 01.10.2017, p. 2413-2432.

Research output: Contribution to journalArticle

TY - JOUR

T1 - POLYGALACTURONASE INVOLVED IN EXPANSION3 functions in seedling development, rosette growth, and stomatal dynamics in Arabidopsis thaliana

AU - Rui, Yue

AU - Xiao, Chaowen

AU - Yi, Hojae

AU - Kandemir, Baris

AU - Wang, James

AU - Puri, Virendra

AU - Anderson, Charles T.

PY - 2017/10/1

Y1 - 2017/10/1

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